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Journal of Plant Biology

, Volume 58, Issue 3, pp 183–192 | Cite as

Epigenetic floral homeotic mutation in pD991-AP3-derived T-DNA-tagged lines for CTP:Phosphorylcholine cytidylyltransferase (CCT) Genes: The homeotic mutation of the cct1-1 allele is enhanced by the cct2 allele and alleviated by CCT1 overexpression

  • Yoshinori Hayakawa
  • Zhongrui Duan
  • Miki Yadake
  • Jun Tsukano
  • Yasuyo Yamaoka
  • Rie Inatsugi
  • Yuki Fujiki
  • Akira Oikawa
  • Kazuki Saito
  • Ikuo NishidaEmail author
Original Article

Abstract

The apetala3 (ap3)-like homeotic mutation (ap3-HM) is recognized among pD991-AP3-derived Arabidopsis thaliana T-DNA-tagged lines carrying the -448 to +47 region of AP3 in their T-DNA. In the corresponding mutant lines for CTP:phosphorylcholine cytidylyltransferase genes, cct1-1 and cct2 (Inatsugi et al. 2009), some flowers of cct1-1 (F4) and many flowers of cct1-1 cct2 (F3) showed ap3-HM, and all flowers of cct1-1 (F5) and cct1-1 cct2 (F4) became increasingly homeotic. In contrast, cct2 flowers were normal for all generations tested. These results demonstrated that ap3-HM is linked to the cct1-1 allele and is enhanced by the cct2 allele. The ap3-HM in cct mutants was inversely correlated with AP3 transcript levels in enriched flower buds. Bisulfite sequencing revealed severe methylation within endogenous AP3 promoter regions in cct1-1 (F3; -317 to -2) and cct1-1 cct2 (F3; -473 to -2), but wild-type (Wassilevskaja) and cct2 plants showed no corresponding methylation. The ap3-HM in cct1-1 cct2 mutants was fully rescued by expressing a PISTILLATA promoter–AP3 construct, and was better alleviated in the F1 offspring of a cross with the CCT1-overexpressing mutant cct1-2 (Columbia) than with the wild type. We discuss possible links between expression of CCT and suppression of ap3-HM.

Keywords

CTP:phosphorylcholine cytidylyltransferase DNA methylation Floral homeotic mutation Homology-dependent gene silencing 

Abbreviation

AP3

APETALA3

CCT, CTP

phosphorylcholine cytidylyltransferase

Col

Columbia

GFP

green fluorescent protein

HDGS

homologydependent gene silencing

PI

PISTILLATA

ProAP3

AP3 promoter

ProPI

PI promoter

Pro35S

Cauliflower mosaic virus 35S promoter

siRNA

small interfering RNA

WS

Wassilevskaja

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Supplementary material

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Copyright information

© Korean Society of Plant Biologists and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Yoshinori Hayakawa
    • 1
  • Zhongrui Duan
    • 1
  • Miki Yadake
    • 1
  • Jun Tsukano
    • 1
  • Yasuyo Yamaoka
    • 1
  • Rie Inatsugi
    • 2
  • Yuki Fujiki
    • 1
  • Akira Oikawa
    • 3
  • Kazuki Saito
    • 3
  • Ikuo Nishida
    • 1
    Email author
  1. 1.Laboratory of Plant Molecular Physiology, Division of Life Science, Graduate School of Science and EngineeringSaitama UniversitySaitamaJapan
  2. 2.Departmenet of Biological Sciences, Graduate School of ScienceThe University of TokyoTokyoJapan
  3. 3.Center for Sustainable Resource ScienceRIKENYokohama, KanagawaJapan

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