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Increased expression of mitochondria-localized carbonic anhydrase activity resulted in an increased biomass accumulation in Arabidopsis thaliana

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Abstract

Carbonic anhydrase (CA; EC 4.2.1.1) catalyzes the interconversion of CO2 and HCO3 - and plays an important role in photosynthetic carbon assimilation. We report that the effect of manipulating AtβCA6 expression on the growth and biomass accumulation of Arabidopsis thaliana shows that AtβCA6 was expressed in mitochondria. Overexpression of AtβCA6 increased the fresh weight, dry weight and rosette leaf area and it was also linked to a slight decrease in the rate of respiration. By contrast, when the respiration rate in the AtβCA6 knock-out mutant SALK_065611 increased, the fresh weight, dry weight and rosette leaf area decreased. The expression level of AtβCA6 mainly affected the expression of the genes that were related to metabolism, photosynthesis and respiration. We discuss these data with respect to a potential role of AtβCA6 in refixation of CO2 released from respiration and its potential as an option to increase biomass production.

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Authors and Affiliations

  1. Key Lab of Plant Stress Research, Life Science College, Shandong Normal University, 88 Wenhua East Road, Jinan, 250014, China

    Chunyun Jiang, Hui Zhang & Yanxiu Zhao

  2. Partner Institute for Computational Biology, Chinese Academy of Sciences, CAS-MPG Partner Institute of Computational Biology, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Danny Tholen, Jiajia Mercedes Xu, Changpeng Xin & Xinguang Zhu

  3. Institute of Botany, Department of Integrative Biology, University of Natural Resources and Applied Life Sciences, BOKU Vienna, Gregor Mendel-Str. 33, A-1180, Vienna, Austria

    Danny Tholen

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  1. Chunyun Jiang
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  2. Danny Tholen
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  3. Jiajia Mercedes Xu
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  4. Changpeng Xin
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  5. Hui Zhang
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  6. Xinguang Zhu
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Correspondence to Xinguang Zhu or Yanxiu Zhao.

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Jiang, C., Tholen, D., Xu, J.M. et al. Increased expression of mitochondria-localized carbonic anhydrase activity resulted in an increased biomass accumulation in Arabidopsis thaliana . J. Plant Biol. 57, 366–374 (2014). https://doi.org/10.1007/s12374-014-0330-8

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  • DOI: https://doi.org/10.1007/s12374-014-0330-8

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