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Molecular evidence for hybrid origin of Aster chusanensis, an endemic species of Ulleungdo, Korea

Journal of Plant Biology volume 57, pages 174–185 (2014)Cite this article

Abstract

Ulleungdo is a small volcanic island and harbors many endemic plant species. Aster chusanensis Lim et al., a recently discovered endemic species on the island was proposed to be derived from hybridization between A. pseudoglehnii Lim et al. and A. oharai Nakai. To test hybrid origin of A. chusanensis, we sequenced the nrITS and three non-coding regions of a plastid genome (trnL-F, rbcL-accD, and psbA-trnH) from eight accessions of A. chusanensis and 37 accessions of its putative parents. Extensive investigation of the genome of A. chusanensis using 160 nrITS PCR amplicon sequences confirmed co-occurrence of nrITS sequences of the parental species within all accessions sampled. The retention of intact parental nrITS types in the genomes of A. chusanensis suggests that hybrid speciation has occurred recently on the island. The plastid DNA sequence data indicate that all of the hybrid individuals inherit the plastid from A. pseudoglehnii, except for one, which has the plastid of A. oharai, implying bidirectional but highly biased hybridization events during the evolution of A. chusanensis. Results of this study suggest that hybridization is an important process in the diversification of indigenous plants on Ulleungdo.

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Authors and Affiliations

  1. Department of Biology, Sooncheonhyang University, Asan, Chungcheongnam-Do, 336-745, Korea

    Hyunchur Shin

  2. Department of Biology, Daejeon University, Daejeon, 300-716, Korea

    Sang-Hun Oh

  3. Research Institute of Northeastern Asian Plants, Seoul, 135-943, Korea

    Yongseok Lim

  4. Plant Resources Division, National Institute of Biological Resources, Incheon, 404-170, Korea

    Chang-Woo Hyun

  5. Department of Life Science, Hallym University, Chuncheon, Gangwon-Do, 200-702, Korea

    Seong-Hyun Cho, Yong-In Kim & Young-Dong Kim

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  1. Hyunchur Shin
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  2. Sang-Hun Oh
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  3. Yongseok Lim
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Shin, H., Oh, SH., Lim, Y. et al. Molecular evidence for hybrid origin of Aster chusanensis, an endemic species of Ulleungdo, Korea. J. Plant Biol. 57, 174–185 (2014). https://doi.org/10.1007/s12374-014-0135-9

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  • DOI: https://doi.org/10.1007/s12374-014-0135-9

Keywords

  • Aster oharai
  • Aster pseudoglehnii
  • Endemic species
  • Hybrid speciation
  • Oceanic island
  • Recombinant nrITS