Journal of Plant Biology

, Volume 56, Issue 1, pp 59–68 | Cite as

Post-meiotic deficient anther1 (PDA1) encodes an ABC transporter required for the development of anther cuticle and pollen exine in rice

  • Lu Zhu
  • Jianxin Shi
  • Guochao Zhao
  • Dabing Zhang
  • Wanqi Liang
Original Article

Abstract

The tapetum of the anther locule encloses the male reproductive cells and plays a supportive role for normal pollen development. However, the underlying mechanism remains less understood. Previously, we identified a complete recessive male sterile mutant, post-meiotic deficient anther1 (pda1), with abnormal postmeiotic tapetal development. In this study we comprehensively characterized pda1. Chemical analysis uncovered that pda1 anther had significant lower levels of cutin monomers and cuticular waxes. PDA1 gene encodes an ATP-binding cassette (ABC) half-transporter, namely OsABCG15, which is conserved from algae to higher plants. In situ RNA hybridization assay showed that PDA1 is strongly expressed in tapetal cells, and weakly in microspores during the anther development. Additionally, the expression of two pollen exine biosynthetic genes CYP704B2 and CYP703A3 was dramatically reduced in pda1 mutant anthers. Altogether, these observations suggest that the tapetum-expressed ABC transporter PDA1 plays a crucial role in secreting lipidic precursors from the tapetum to developing microspores and the anther epidermis.

Keywords

ABCG transporter Exine Lipidic precursors Oryza sativa Tapetum 

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Copyright information

© Korean Society of Plant Biologists and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Lu Zhu
    • 1
  • Jianxin Shi
    • 1
  • Guochao Zhao
    • 1
  • Dabing Zhang
    • 1
  • Wanqi Liang
    • 1
  1. 1.State Key Laboratory of Hybrid Rice, School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina

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