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Taxonomic Status and Genetic Variation of Korean Endemic Plants, Eranthis byunsanensis and Eranthis pungdoensis (Ranunculaceae) based on nrDNA ITS and cpDNA Sequences

Journal of Plant Biology volume 55, pages 165–177 (2012)Cite this article

Abstract

To reappraise the taxonomic status of two Korean endemic plants, Eranthis byunsanensis and Eranthis pungdoensis, we analyzed five taxa of that genus plus three out-group genera (Cimicifuga, Anemonopsis, and Anemone). In all, 52 representative accessions were examined for nrDNA (ITS) and 43 accessions for cpDNA (trnH–psbA, rps16, trnL, and trnLF). In the ITS region, all of the aligned sequences from E. byunsanensis had the same ribotype as from E. pungdoensis. For the ITS phylogeny, E. pungdoensis could not be distinguished from E. byunsanensis. However, the clade of E. byunsanensis, including E. pungdoensis, was separated from Eranthis pinnatifida of Japan, which has a distinct ribotype and forms a sister group. In our cpDNA analysis, E. byunsanensis showed paraphyly, and the clade of E. pungdoensis was nested within the E. byunsanensis clade. Haplotypes of each population of E. byunsanensis were highly variable, and TCS analysis of that species implied that the Jeju population is ancestral. Moreover, the cp-group of E. pungdoensis was separated from the other cp-groups by five substitutions and five indels. Therefore, based on these DNA data and TCS analysis, we advise that the taxonomic status of E. pungdoensis be treated as E. byunsanensis B. Sun var. pungdoensis (B.U. Oh) S.H. Yeau, C.S. Lee & N.S. Lee, stat. nov.

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Acknowledgments

This research was supported by an RP-Grant 2010 from Ewha Womans University. We thank all of the unknown reviewers for their comments.

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Authors and Affiliations

  1. Research Institute of EcoScience, Ewha Womans University, Seoul, 120-750, South Korea

    Chang Shook Lee

  2. Department of Science Education, Ewha Womans University, Seoul, 120-750, South Korea

    Sung Hee Yeau

  3. Department of Life Science, Ewha Womans University, Seoul, 120-750, South Korea

    Nam Sook Lee

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  1. Chang Shook Lee
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Correspondence to Sung Hee Yeau or Nam Sook Lee.

Appendix 1. Voucher information for E. byunsanensis and related taxa as part of their molecular and morphological analyses. Abbreviations for sampling locations are shown in Fig. 1—data not available; *sequence and scientific name from GenBank (Accession Numbers for ITS /trnH–psbA /rps16 /trnL and trnLF are shown after each taxon)

Appendix 1. Voucher information for E. byunsanensis and related taxa as part of their molecular and morphological analyses. Abbreviations for sampling locations are shown in Fig. 1—data not available; *sequence and scientific name from GenBank (Accession Numbers for ITS /trnH–psbA /rps16 /trnL and trnLF are shown after each taxon)

Taxon, DNA Accession Number, Location for sampling, Voucher specimens, GenBank Accession Number

E. byunsanensis B. Y. Sun, 32-33, SEO, Korea, GW, Seoraksan, S. Yeau 0504021-22, JF505768-9/JF505810-11/JF505852-3/JF505894-5; 26-27, SUR, Korea, GG, Anyang-si, Surisan, N. Lee 0703030-31, JF505770-1/JF5058012-13/JF505854-5/JF505896-7; 35-36, NEB, Korea, JB, Naebyeonsan, S. Yeau 0703011-12, JF505772-3/JF505814-15/JF505856-7/JF505898-9; 18-20, 37, JIN, Korea, JB, Jinan, Maisan, S. Yeau 05030010-12, S. Yeau 0603015, JF505774-7/JF505816-9/JF505858-61/JF505900-3; 21-22, NAJ, Korea, JB, Naejangsan, S. Yeau 05030013-14, JF505778-9/JF505820-1/JF505862-3/JF505904-5; 23, GAN, Korea, JN, Gangjin-gun, Gangjin, S. Yeau 05030021, JF505780/JF505822/JF505864/JF505906; 29-30, GYE, Korea, KB, Gyeongju, Y. Kim 0504005-6, JF505781-2/JF505823-4/JF505865-6/JF505907-8; 1-4, YEO, Korea, JN, Yeosu, Hyangilam, S. Yeau 0403001-4, JF505786-9/JF505828-31/JF505870-3/JF505912-5; 49-50, 53, MAS, Korea, KN, Masan, Subalsan, S. Yeau 0803001-3, JF505783-5/JF505825-7/JF505867-9/JF505909-11; 6, 7, 10, JEU, Korea, JJ, Sangumburi, S. Yeau et al. 0403015-17, JF505790-2/JF505832-34/JF505874-6/JF505916-8; E. pungdoensis B. U. Oh, 24, 25, 38-40, 47, 48, 52, PUN, Korea, GG, Ansan-si, Isl. Pungdo, N. Lee et al. 0503018-19/C. Lee & M. Cha 0605005-10, JF505793-800/JF505835-42/JF505877-84/JF505919-26; E. pinnatifida Maxim., 13-17, Japan, Mt. Fujiwara, N. Lee & S. Yeau 0103002-6, JF505801-5/JF505843-47/JF505885-89/JF505927-31; 28, Japan, Bot. Garden of Koishikawa, cult., D. Suk, JF505806/JF505848/JF505890/JF505932; *-, Japan, - , AJ496615/-/-/-; E. stellata Maxim., 11-12, Korea, Jeonbuk, Jeokseongsan (Dukyusan), S. Yeau 0404017-18, JF505766-7/JF505808-9/JF505850-1/JF505892-3; *2006-0353, Korea, - , FJ597992/-/-/-; E. hyemalis Salisb, 45, USA, Brooksides Garden, MD, S. Roh, JF505765/JF505807/JF505849/JF505891; UK, England, J. A. Compton et al., Z98273/-/-/-; France, Paris, Jardin des Plantes, L. Despres et al., AY148284/-/-/-; Anemone pendulisepala Y. Lee, 15, GW, Taebaeksan, N. Lee et al. 040501, EF139253/EF139289/EF139361/ EF139325; *Anemonopsis macrophylla Siebold & Zucc., UK, England, J. A. Compton et al., Z98275/-/-/-; *Cimicifuga acerina (Siebold. & Zucc.) Tanaka, China, Mt. Jinfo, Nanachuan, Wang 90131, AF055040, AF055041/-/-/-; *Cimicifuga nanchuanensis Hsiao, China, Sichuan Province, Fushimi et al. AB029378/-/-/-; *Cimicifuga japonica Spreng, Korea, JJ, CD&C 266 (Cult. 1993. RNG: Compton et al.), Z98292/-/-/-

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Lee, C.S., Yeau, S.H. & Lee, N.S. Taxonomic Status and Genetic Variation of Korean Endemic Plants, Eranthis byunsanensis and Eranthis pungdoensis (Ranunculaceae) based on nrDNA ITS and cpDNA Sequences. J. Plant Biol. 55, 165–177 (2012). https://doi.org/10.1007/s12374-011-9201-8

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