Establishment of Glucocorticoid-Mediated Transcriptional Induction of the Rice XA21 Pattern Recognition Receptor
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The rice pattern recognition receptor, XA21, confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). We have generated transgenic plants that express Xa21 only in the presence of the glucocorticoid hormone, dexamethasone (DEX). DEX-mediated transcriptional induction of Xa21 is accompanied by upregulation of pathogenesis-related 1 gene expression and restriction of Xoo multiplication. The DEX-inducible system can be used to synchronize the XA21-mediated response to infection throughout the entire leaf, facilitating the study of innate immunity.
KeywordsDexamethasone Glucocorticoid Oryza sativa Pattern recognition receptor XA21
GAL4-binding domain–VP16 activation domain–GR fusion
Pattern recognition receptor
Xanthomonas oryzae pv. oryzae
We thank Dr. Sang-Wook Han and Dr. Ofir Bahar for critical reading of the manuscript, Dr. Mawsheng Chen for the Gateway-compatible pTA7002 vector, and Randy Ruan for lab and greenhouse management. We also thank Seo Jung Yang and Timothy Tong for the technical assistance. This work was supported by the National Institute of Health (NIH, GM55962) and the National Science Foundation (NSF, IOS-0817738).
Conflict of Interest
The authors declare no competing financial interests.
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