Abstract
The potential for global warming and climate change has increased the focus of research on plant genes that respond to high temperatures. Previous research identified a temperature-sensitive miniature soybean mutant that was controlled by a single gene. The objectives of our research were to confirm the single-gene control and to determine the genomic location of this gene. Segregation of the combined progeny of four BC6F5 plants heterozygous for the miniature trait in a Tracy-M background confirmed that the trait was conditioned by a single gene (1:2:1, χ 2 = 4.38, P = 0.1120). Molecular marker analysis identified three SSR markers and a SNP marker on molecular linkage group B2 (chromosome 14) associated with segregation for the miniature trait. One of these, marker Satt560, co-segregated perfectly with the miniature trait. The data from these four polymorphic markers indicated that the gene conditioning this miniature phenotype is at or near Satt560. Given this newly identified location of the gene and the recently published soybean genomic sequence, it may be feasible to isolate the gene and determine its mechanism of action in responding to temperature. Such knowledge may be of use in understanding how plants respond to increased temperature.
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Acknowledgments
This work was supported by United States Department of Agriculture–Agriculture Research Service (USDA-ARS) project nos. 6402-21220-010-00D and 6645-21000-026-00D. The authors wish to thank Dr. T. C. Kilen, USDA-ARS, retired, who provided the seed used in this study, technical knowledge for working with miniature plants, and information on population development.
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Ray, J.D., Smith, J.R., Taliercio, E. et al. Genomic Location of a Gene Conditioning a Miniature Phenotype in Soybean [Glycine max (L.) Merr.]. J. Plant Biol. 55, 26–32 (2012). https://doi.org/10.1007/s12374-011-9186-3
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DOI: https://doi.org/10.1007/s12374-011-9186-3