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F-box Protein Arabidillo-1 Promotes Lateral Root Development by Depressing the Functioning of GA3 in Arabidopsis

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Abstract

In Arabidopsis, Arabidillo-1 and Arabidillo-2 have great sequence homology to Dictyostelium and metazoan β–catenin/Armadillo, which are important to animal and Dictyostelium development. Arabidillo-1 and Arabidillo-2 promote lateral root formation redundantly in Arabidopsis. Here, we showed that gibberellins (GA3) has a greater inhibitory effect on lateral root growth from the null mutant arabidillo-1 than from the wild type, suggesting that the mechanism for Arabidillo-1-regulated modulation of lateral root proliferation is associated with GA3-metabolic or signaling pathways. Our yeast two-hybrid analysis demonstrated that Arabidillo-1 interacts with ASK2 and ASK11, and that ASK2 can bind with the F-box domain of Arabidillo-1. Therefore, Arabidillo-1 is involved in the ubiquitin/26S proteasome-mediated proteolytic pathway. Based on these results, we conclude that Arabidillo-1 can degrade some positive regulator of the GA3 signaling pathway through selective protein degradation of ubiquitin/26S. Moreover, that process is believed to be the mechanism for Arabidillo-1 promotion of lateral root development in Arabidopsis.

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Acknowledgment

This work was financially supported by grants received under the National Natural Science Foundation of China (No.30771091) and the Key Grant Project of Chinese Ministry of Education (No.307024).

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Authors and Affiliations

  1. Institute of Cell Biology, School of Life Science, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Changjun Mu, Ni Chen, Xiaofeng Li, Pengfei Jia & Heng Liu

  2. School of Pharmaceutical, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Zhaoyan Wang

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  1. Changjun Mu
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  2. Ni Chen
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  3. Xiaofeng Li
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  4. Pengfei Jia
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  5. Zhaoyan Wang
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  6. Heng Liu
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Correspondence to Heng Liu.

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Mu, C., Chen, N., Li, X. et al. F-box Protein Arabidillo-1 Promotes Lateral Root Development by Depressing the Functioning of GA3 in Arabidopsis . J. Plant Biol. 53, 374–380 (2010). https://doi.org/10.1007/s12374-010-9125-8

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