Abstract
A developmentally stunted mutant (dsm1) of Arabidopsis, isolated from an EMS mutant screen, had a pleiotropic phenotype, including repressed germination, retarded growth, delayed flowering, and impaired fertility. Additionally, dsm1 had a lifespan of approximately 160 days, which was more than twice the lifespan of the wild type (Col-0). Fine morphological and anatomical characters, such as the shoot apical meristem, root apical meristem, seed shape, and seed surface, were obviously altered in dsm1. We found that both abscisic acid and zeatin riboside levels were significantly greater in dsm1 than in Col-0 at all stages of development, while the levels of indole-3-acetic acid and gibberellins varied by age. The expressions of some abscisic acid-related genes were higher in dsm1 than in Col-0. These data indicate that DSM1 may play a general role in plant growth and development.
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References
Bowman JL, Smyth DR, Meyerowitz EM (1989) Genes directing flower development in Arabidopsis. Plant Cell 1(1):37–52
Boyes DC, Zayed AM, Ascenzi R, McCaskill AJ, Hoffman NE, Davis KR, Gorlach J (2001) Growth stage-based phenotypic analysis of Arabidopsis: a model for high throughput functional genomics in plants. Plant Cell 13(7):1499–1510
Brocard-Gifford I, Lynch TJ, Garcia ME, Malhotra B, Finkelstein RR (2004) The Arabidopsis thaliana ABSCISIC ACID-INSENSITIVE8 encodes a novel protein mediating abscisic acid and sugar responses essential for growth. Plant Cell 16(2):406–421
De Veylder L, Beeckman T, Beemster GT, Krols L, Terras F, Landrieu I, van der Schueren E, Maes S, Naudts M, Inze D (2001) Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis. Plant Cell 13:1653–1668
Finkelstein R, Rock C (2002) Abscisic acid biosynthesis and signaling. In: Somerville CR, Meyerowitz EM (eds) The Arabidopsis book ed, Vol. American Society of Plant Biologists, Rockville
Frey A, Boutin JP, Sotta B, Mercier R, Marion-Poll A (2006) Regulation of carotenoid and ABA accumulation during the development and germination of Nicotiana plumbaginifolia seeds. Planta 224(3):622–632
Fujiwara T, Nambara E, Yamagishi K, Goto DB, Naito S (2002) Storage proteins. In: Somerville CR, Meyerowitz EM (eds) The Arabidopsis book, ed, vol. American Society of Plant Biologists, Rockville
He Z (1993) Guidance to experiment on chemical control in crop plants. In: He ZP (ed) Guidance to experiment on chemical control in crop plants. Beijing Agricultural University Publishers, Beijing, pp 60–68
Kinoshita T, Yadegari R, Harada JJ, Goldberg RB, Fischer RL (1999) Imprinting of the MEDEA polycomb gene in the Arabidopsis endosperm. Plant Cell 11(10):1945–1952
Le Hir R, Leduc N, Jeannette E, Viemont JD, Pelleschi-Travier S (2006) Variations in sucrose and ABA concentrations are concomitant with heteroblastic leaf shape changes in a rhythmically growing species (Quercus robur). Tree Physiol 26(2):229–238
Lefebvre V, North H, Frey A, Sotta B, Seo M, Okamoto M, Nambara E, Marion-Poll A (2006) Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy. Plant J 45(3):309–319
Leung J, Giraudat J (1998) Abscisic acid signal transduction. Annu Rev Plant Physiol Plant Mol Biol 49:199–222
McNellis TW, Deng XW (1995) Light control of seedling morphogenetic pattern. Plant Cell 7(11):1749–1761
Millar AA, Jacobsen JV, Ross JJ, Helliwell CA, Poole AT, Scofield G, Reid JB, Gubler F (2006) Seed dormancy and ABA metabolism in Arabidopsis and barley: the role of ABA 8′-hydroxylase. Plant J 45(6):942–954
Penfield S, Pinfield-Wells HM, Graham IA (2006) Storage reserve mobilisation and seedling establishment in Arabidopsis. In: Somerville CR, Meyerowitz EM (eds) The Arabidopsis book, ed, vol. American Society of Plant Biologists, Rockville
Peng Z, Zhou X, Li L, Yu X, Li H, Jiang Z, Cao G, Bai M, Wang X, Jiang C, Lu H, Hou X, Qu L, Wang Z, Zuo J, Fu X, Su Z, Li S, Guo H (2009) Arabidopsis hormone database: a comprehensive genetic and phenotypic information database for plant hormone research in Arabidopsis. Nucleic Acids Res 37(suppl_1):D975–D982
Piskurewicz U, Jikumaru Y, Kinoshita N, Nambara E, Kamiya Y, Lopez-Molina L (2008) The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity. Plant Cell 20(10):2729–2745
Rabino I, Mancinelli AL (1986) Light, temperature, and anthocyanin production. Plant Physiol 81(3):922–924
Ren GD, An K, Liao Y, Zhou X, Cao YJ, Zhao HF, Ge XC, Kuai BK (2007) Identification of a novel chloroplast protein AtNYE1 regulating chlorophyll degradation during leaf senescence in Arabidopsis. Plant Physiol 144(3):1429–1441
Rock C (2000) Pathways to abscisic acid-regulated gene expression. New Phytol 148:357–396
Rohde A, Kurup S, Holdswoth M (2000) ABI3 emerges from the seed. Trends Plant Sci 5(10):418–419
Shinozaki K, Yamaguchi-Shinozaki K (2000) Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol 3(3):217–223
Wang H, Qi Q, Schorr P, Cutler AJ, Crosby WL, Fowke LC (1998) ICK1, a cyclin-dependent protein kinase inhibitor from Arabidopsis thaliana interacts with both Cdc2a and CycD3, and its expression is induced by abscisic acid. Plant J 15(4):501–510
Wang H, Zhou Y, Gilmer S, Whitwill S, Fowke LC (2000) Expression of the plant cyclin-dependent kinase inhibitor ICK1 affects cell division, plant growth and morphology. Plant J 24(5):613–623
Zhu Y, Zhao HF, Ren GD, Yu XF, Cao SQ, Kuai BK (2005) Characterization of a novel developmentally retarded mutant (drm1) associated with the autonomous flowering pathway in Arabidopsis. Cell Res 15(2):133–140
Acknowledgments
Mutant screening was conducted at the Dong Lab of the Developmental, Cell and Molecular Biology group, Department of Biology, Duke University. We are grateful to Professor Xinnian Dong and Professor Zhongnan Yang for their valuable advice and help. We would also like to thank Drs. Jufang Gao, Jun Zhu, and Yongyou Zhu for their technical assistance.
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Supported by the National Natural Science Foundation of China (39870452).
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Zhao, HF., Qiu, K., Ren, GD. et al. A Pleiotropic Phenotype is Associated with Altered Endogenous Hormone Balance in the Developmentally Stunted Mutant (dsm1). J. Plant Biol. 53, 79–87 (2010). https://doi.org/10.1007/s12374-009-9090-2
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DOI: https://doi.org/10.1007/s12374-009-9090-2