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A Defect in Zinc Finger Protein Double B-box 1a (DBB1a) Causes Abnormal Floral Development in Arabidopsis

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Abstract

The double B-box (DBB) type zinc finger protein has thus far been shown to be involved in photomorphegenesis in Arabidopsis thaliana. Here, we show that DBB1a is expressed in the embryo, cytolden, and flower. Misexpression of DBB1a in mutant plants resulted in abnormal numbers and patterns of floral organs. We further show that DBB1a could regulate expression of several floral homeotic genes, including APETALA 2, APETALA 3, PISTILLATA, and AGAMOUS. Interestingly, expression of the microRNA gene MiR172, which is involved in organ boundary establishment, was also misregulated in the dbb1a mutant plants. Our study identified a previously uncharacterized role of DDB1a in regulation of expression of floral homeotic genes and miR172, which is important for understanding of floral pattern formation.

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Acknowledgments

Thanks to Dr. Bekir Ulker and Dr. JaneParker for kindly providing pJawohl8-RNAi, and Dr. Ying Ruan and Wenhui Shen for his critical reading of the manuscript. This research was supported by the grants from National Natural Science Foundation of China (30600368; 30770200; 30871325).

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Authors and Affiliations

  1. Institute of Life Science and Biotechnology, Hunan University, Changsha, 410082, Hunan Province, People’s Republic of China

    Qiming Wang, Xiaoju Tu, Keqin Deng, Jianxin Zeng, Xiaoying Zhao, Dongying Tang & Xuanming Liu

  2. State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, 410082, Hunan Province, People’s Republic of China

    Qiming Wang, Keqin Deng, Jianxin Zeng & Xuanming Liu

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  1. Qiming Wang
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  2. Xiaoju Tu
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Correspondence to Xuanming Liu.

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Wang, Q., Tu, X., Deng, K. et al. A Defect in Zinc Finger Protein Double B-box 1a (DBB1a) Causes Abnormal Floral Development in Arabidopsis . J. Plant Biol. 52, 543–549 (2009). https://doi.org/10.1007/s12374-009-9070-6

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