Abstract
Oligofructans are a combination of fructo-oligosaccharides and fructose polymers that are linked by β-glycosidic bonds. Because of their distinctive properties and potential health benefits, they are widely used in many industries. This study optimized microbial oligofructans fermentation using Bacillus subtilis TISTR 001. Fermentative parameters (pH, temperature, shaking rate, sucrose concentration, and fermentation time) were investigated in a one-factor-at-a-time (OFAT) experiment using surface response methodology to determine the predicted model and optimization. Different carbon sources (sucrose, raw sugar, sugarcane juice, and molasses) were studied under the optimized conditions. The results from the Box–Behnken experimental design with four factors and three levels and the predicted model showed the optimum fermentation conditions were 35°Brix of substrate concentration at pH 6.5, 32.5 °C, and 250 rpm shaking speed for 48 h to obtain 88.64 g/L of oligofructans. The predicted oligofructans content (88.64 g/L) was higher than the actual production using sucrose (75.86 g/L), which was the best substrate for oligofructans production. Raw sugar and sugarcane juice could be alternative substrates for oligofructans production based on these optimized conditions, while molasses had the lowest production due to its impurities that negatively affected oligofructans production.
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References
Bersaneti, G.T., N.C. Pan, C. Baldo, and M.A.P.C. Celligoi. 2018. Coproduction of fructooligosaccharides and levan by levansucrase from Bacillus subtilis natto with potential application in the food industry. Applied Biochemistry and Biotechnology 184: 838–851. https://doi.org/10.1007/s12010-017-2587-0.
Chidambaram, J.S.C., B. Veerapandian, K.K. Sarwareddy, K.P. Mani, S.R. Shanmugam, and P. Venkatachalam. 2019. Studies on solvent precipitation of levan synthesized using Bacillus subtilis MTCC 441. Heliyon 5 (9): e02414. https://doi.org/10.1016/j.heliyon.2019.e02414.
Da Silva, B., D. Borsato, and M.A.P.C. Celligoi. 2014. Optimization of high production of fructooligosaccharides by sucrose fermentation of Bacillus subtilis natto CCT7712. American Journal of Food Technology 9 (3): 144–150. https://doi.org/10.3923/ajft.2014.144.150.
De Oliveira, M.R., R.S.S.F. Da Silva, J.B. Buzato, and M.A.P.C. Celligoi. 2007. Study of levan production by Zymomonas mobilis using regional low-cost carbohydrate sources. Biochemical Engineering Journal. 37 (2): 177–183. https://doi.org/10.1016/j.bej.2007.04.009.
Deng, S.P., and M.A. Tabatabai. 1994. Colorimetric determination of reducing sugars in soils. Soil Biology and Biochemistry 26 (4): 473–477. https://doi.org/10.1016/0038-0717(94)90179-1.
Gamal, A.A., H.Y. Abbas, N.A.M. Abdelwahed, M.T. Kashef, K. Mahmoud, and M.A. Esawy. 2021. Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment. International Journal of Biological Macromolecules 182: 1590–1601. https://doi.org/10.1016/j.ijbiomac.2021.05.056.
Goldman, D., N. Lavid, A. Schwartz, G. Shoham, D. Danino, and Y. Shoham. 2008. Two active forms of Zymomonas mobilis levansucrase: An ordered microfibril structure of the enzyme promotes levan polymerization. Journal of Biological Chemistry 283 (47): 32209–32217. https://doi.org/10.1074/jbc.M805985200.
Goncalves, B.C.M., J. Mantovan, M.L.L. Ribeiro, D. Borosato, and M.A.P.C. Celligoi. 2013. Optimization production of thermo active levansucrase from Bacillus subtilis Natto CCT7712. Journal of Applied Biology 1: 001–008. https://doi.org/10.7324/JABB.%202013.1201.
Han, J., H. Feng, X. Wang, Z. Liu, and Z. Wu. 2021. Levan from Leuconostoc citreum BD1707: Production optimization and changes in molecular weight distribution during cultivation. BMC Biotechnology 21 (1): 1–14. https://doi.org/10.1186/s12896-021-00673-y.
Khassaf, W.H., A.K. Niamah, and A.J. Al-Manhel. 2019. Study of the optimal conditions of levan production from a local isolate of Bacillus subtilis subsp. subtilis w36. Basrah Journal of Agricultural Sciences 32 (2): 213–222. https://doi.org/10.37077/25200860.2019.211.
Magri, A., M.R. Oliveira, C. Baldo, C.A. Tischer, D. Sartori, M.S. Mantovani, and M.A.P.C. Celligoi. 2020. Production of fructooligosaccharides by Bacillus subtilis natto CCT7712 and their antiproliferative potential. Journal of Applied Microbiology 128 (5): 1414–1426. https://doi.org/10.1111/jam.14569.
Mummaleti, G., C. Sarma, S.K. Kalakandan, H. Gazula, V. Sivanandham, and A. Anandharaj. 2022. Characterization of levan produced from coconut inflorescence sap using Bacillus subtilis and its application as a sweetener. LWT. 154: 112697. https://doi.org/10.1016/j.lwt.2021.112697.
Nelson, N. 1944. A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153(2): 375–380. https://doi.org/10.1016/S0021-9258(18)71980-7
Ninchan, B., and C. Noidee. 2021a. Optimization of oligofructans production from sugarcane juice fermentation using Bacillus subtilis TISTR001. Agriculture and Natural Resources 55 (6): 1005–1014. https://doi.org/10.34044/j.anres.2021.55.6.11.
Ninchan, B., and C. Noidee. 2021b. Production and prebiotic properties of oligofructans from sugarcane juice fermentation by Bacillus subtilis TISTR001. 3 Biotech 11 (5): 213. https://doi.org/10.1007/s13205-021-02757-0.
Noidee, C., S. Songbang, and B. Ninchan. 2023. Comparative efficiency of oligofructans production by Bacillus subtilis TISTR 001 from different carbon sources: Sucrose, sugarcane juice, and molasses. Sugar Tech 25 (4): 950–958. https://doi.org/10.1007/s12355-023-01245-4.
Olmos, J., and J. Paniagua-Michel. 2014. Bacillus subtilis a potential probiotic bacterium to formulate functional feeds for aquaculture. Journal of Microbial & Biochemical Technology 6 (7): 361–365. https://doi.org/10.4172/1948-5948.1000169.
Phengnoi, P., N. Thakham, T. Rachphirom, N. Teerakulkittipong, G.A. Lirio, and W. Jangiam. 2022. Characterization of levansucrase produced by novel Bacillus siamensis and optimization of culture condition for levan biosynthesis. Heliyon 8 (12): e12137. https://doi.org/10.1016/j.heliyon.2022.e12137.
Puchkova, T.S., M. Daniya, and M. Oksana. 2020. Production of oligofructose syrup by enzymatic hydrolysis from inulin-containing chicory. Food System 3 (3): 20–24. https://doi.org/10.21323/2618-9771-2020-3-3-20-24.
Ramya, P., D. Sangeetha, E.S. Anooj, and L. Gangadhar. 2020. Studies on the production and optimization of levan from Bacillus sp. Annals of Tropical Medicine and Public Health 23: 1188–1197. https://doi.org/10.36295/ASRO.2020.23744.
Shao, Y., and A.H.M. Lin. 2018. Improvement in the quantification of reducing sugars by miniaturizing the Somogyi-Nelson assay using a microtiter plate. Food Chemistry 240: 898–903. https://doi.org/10.1016/j.foodchem.2017.07.083.
Shih, I.L., Y.T. Yu, C.J. Shieh, and C.Y. Hsieh. 2005. Selective production and characterization of levan by Bacillus subtilis (Natto) Takahashi. Journal of Agricultural and Food Chemistry. 53 (21): 8211–8215. https://doi.org/10.1021/jf058084o.
Sumiyanto, J., F.E. Dayan, A.L. Cerdeira, Y.H. Wang, I.A. Khan, and R.M. Moraes. 2012. Oligofructans content and yield of yacon (Smallanthus sonchifolius) cultivated in Mississippi. Scientia Horticulture 148: 83–88. https://doi.org/10.1016/j.scienta.2012.09.020.
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The Sugars and Derivatives Analytical Laboratory (SuDAL), Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand, supported this research.
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Noidee, C., Ninchan, B. Investigation of Optimized Microbial Oligofructans Production by Bacillus subtilis TISTR 001 Using Response Surface Methodology. Sugar Tech 26, 585–594 (2024). https://doi.org/10.1007/s12355-024-01366-4
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DOI: https://doi.org/10.1007/s12355-024-01366-4