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Salt Stress Induces Complicated miRNA Responses of Sugar Beet (Beta vulgaris L.)

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Abstract

Although sugar beet has a high salt tolerance, the quantity and characteristics of miRNAs and their potential effects on salt stress response remain unclear. The leaves of beet variety "N98122" were used for miRNA sequencing at 0, 2.5, 7.5, and 16h. The results showed that small RNAs of 21 nt had the highest abundance, followed by those of 22 and 23 nt. In total, 654 differential miRNAs, comprising 24 known and 630 novel miRNAs, were determined from seedlings treated with NaCl for 0, 2.5, 7.5, and 16h. Of the 24 known miRNAs, 17 exhibited downregulated expression, including miR159, miR166, miR1873, miR319, miR3626, miR3699, and miR397, and seven miRNAs exhibited upregulated expression, including miR156, miR3630, miR481, miR4995, miR5369, miR536, and miR6173. GRF, HKT, PAGR, SDK, Eps1511, VPS, PAN, 1-Sep, CFDP, DRG, DET, IFRD, PSA, mfsd, and ANTR were determined as the target genes of the differentially expressed miRNAs. The outcomes of this study offer important information about miRNAs that respond to salt conditions, and this knowledge may prove useful for enhancing the tolerance of sugar beet to salt stress.

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Data availability

The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive (Genomics, Proteomics & Bioinformatics 2021) in National Genomics Data Center (Nucleic Acids Res 2022), China National Center for Bioinformation / Beijing Institute of Genomics, Chinese Academy of Sciences (GSA: CRA010166) that are publicly accessible at https://ngdc.cncb.ac.cn/gsa.

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Funding

The work was supported by the earmarked fund for CARS (CARS-170104); Inner Mongolia Autonomous Region "the open competition mechanism to select the best candidates" project entitled "Creation of Elite Beet Germplasm and Breeding of Varieties Suitable for Mechanized Operation" (2022JBGS0029); Inner Mongolia key laboratory of sugar beet genetics and germplasm enhancement; Inner Mongolia Natural Science Fund (2023LHMS03031).

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Author notes

  1. Xiaodong Li and Huizhong Zhang both are Co corresponding authors.

Authors and Affiliations

  1. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, 010031, China

    Ziqiang Zhang, Zengjuan Fu, Wenjin Chen, Liang Wang, Shangmin Zhao, E Yuanyuan, Hui Zhang, Bizhou Zhang, Mengyuan Sun, Wenzhe Zheng, Xiaodong Li & Huizhong Zhang

  2. Wuhai Agriculture and Animal Husbandry Bureau, Wuhai, 016000, China

    Jing Dong

  3. Inner Mongolia Hydrology and Water Resources Center, Hohhot, 010020, China

    Zhiqi Zhang

  4. Duolun County People’s Government, Xilin Gol League, 027300, China

    Zhihui Ning

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  1. Ziqiang Zhang
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Contributions

ZZQ contributed to design experiments, stress treatment, analyze data, and write papers; FZJ, CWJ, and WJ involved in data curation and contributed to resources; ZSM, EYY, ZH, ZBZ, and SMY helped to analyze data; ZWZ, DJ, ZZQ, and NZH done partial experiment; LXD and ZHZ involved in argumentation method. All authors contributed to the article and approved the submitted version.

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Correspondence to Ziqiang Zhang.

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Zhang, Z., Fu, Z., Chen, W. et al. Salt Stress Induces Complicated miRNA Responses of Sugar Beet (Beta vulgaris L.). Sugar Tech (2023). https://doi.org/10.1007/s12355-023-01341-5

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  • DOI: https://doi.org/10.1007/s12355-023-01341-5

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