Abstract
The aim of this study was to develop an innovative and functional beverage formulation with probiotic properties using carbon sources such as sucrose, agave syrup, and honey. Analysis showed that lemonade-honey had the highest antioxidant values on day 0, but activity decreased on day 14. The numbers of Lactococcus ssp., Lactobacillus ssp., and total bacteria in lemonade-honey were found to be higher on the days 0 and 7 of fermentation than on day 14. The highest glucose and fructose concentrations and the lowest sucrose concentrations in lemonade-agave syrup and lemonade-honey were on day 7. At the end of fermentation, the highest concentration of vitamin C was detected in the lemonade-agave syrup formulation. All mineral substances analyzed in lemonade-honey reached their highest values on the days 7 and 14 of storage, especially phosphorus, potassium, iron, and zinc. In terms of sensory characteristics, the panelists gave higher scores for lemonade-agave syrup. As a result, all lemonade formulations proved to be suitable for water kefir. However, lemonade-agave syrup and lemonade-honey are recommended to be consumed within the first seven days due to their pleasant aroma, probiotic functional properties, better preservation of bioactive components, and lower acidity.
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References
Aligita, A.W., P.N. Tarigan, and E. Susilawati. 2020. Antiinflamatory and antioxidant activity of kefir water. International Journal of Biology, Pharmacy and Allied Sciences 9 (1): 2454–2464.
Alsayadi, M., Y. Al Jawfi, M. Belarbi, and F.Z. Sabri. 2013. Antioxidant potency of water kefir. Journal of Microbiology, Biotechnology and Food Sciences 2 (6): 2444.
Alvarez-Suarez, J.M., S. Tulipani, D. Díaz, Y. Estevez, S. Romandini, F. Giampieri, E. Damiani, P. Astolfi, S. Bompadre, and M. Battino. 2010. Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with color, polyphenol content and other chemical compounds. Food and Chemical Toxicology 48: 2490–2499.
AOAC (Association of Offıcial Analytical Chemists). 2012. Official methods of analysis of AOAC International, 19th ed. Arlington: Association of official analytical chemists.
Apak, R., K. Güçlü, M. Ozyürek, S.E. Karademir, and E. Erçağ. 2006. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. International Journal of Food Sciences and Nutrition 57: 292–304. https://doi.org/10.1080/09637480600798132.
Araujo, C.S., L.L. Macedo, and L.J.Q. Teixeira. 2023. Use of mid-infrared spectroscopy to predict the content of bioactive compounds of a new non-dairy beverage fermented with water kefir. LWT-Food Science and Technology 176: 114514. https://doi.org/10.1016/j.lwt.2023.114514.
Barreca, D., E. Bellocco, C. Caristi, U. Leuzzi, and G. Gattuso. 2011. Flavonoid profile and radical-scavenging activity of mediterranean sweet lemon (Citrus limetta risso) juice. Food Chemistry 129: 417–422. https://doi.org/10.1016/j.foodchem.2011.04.093.
Brand-Williams, W., M.E. Cuvelier, and C.L.W.T. Berset. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology 28: 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5.
Bueno, R.S., J.B. Ressutte, N.N.Y. Hata, F.C. Henrique-Bana, K.B. Guergoletto, A.G. de Oliveira, and W.A. Spinosa. 2021. Quality and shelf life assessment of a new beverage produced from water kefir grains and red pitaya. LWT-Food Science and Technology 140: 110770. https://doi.org/10.1016/j.lwt.2020.110770.
Coda, R., A. Lanera, A. Trani, M. Gobbetti, and R. Di Cagno. 2012. Yogurt-like beveragesmade of a mixture of cereals, soy and grape must: Microbiology, texture, nutritional and sensory properties. International Journal of Food Microbiology 155: 120–127. https://doi.org/10.1016/j.ijfoodmicro.2012.01.016.
Corona, O., W. Randazzo, A. Miceli, R. Guarcello, N. Francesca, H. Erten, G. Moshetti, and L. Settanni. 2016. Characterization of kefir-like beverages produced from vegetable juices. LWT-Food Science and Technology 66: 572–581. https://doi.org/10.1016/j.lwt.2015.11.014.
Dani, C., L.S. Oliboni, R. Vanderlinde, D. Bonatto, M. Salvador, and J.A.P. Henriques. 2007. Phenolic content andantioxidant activities of white and purple juices manufactured with organically-or conventionally-produced grapes. Food and Chemical Toxicology 45 (12): 2574–2580. https://doi.org/10.1016/j.fct.2007.06.022.
Destro, T.M., D.F. Prates, L.S. Watanabe, S. Garcia, G. Biz, and W.A. Spinosa. 2019. Organic brown sugar and jaboticaba pulp influence on water kefir fermentation. Food Science and Technology 43: 1–16. https://doi.org/10.1590/1413-7054201943005619.
Dordevic, T.M., S.S. Siler-Marinkovic, and S.I. Dimitrijevic-Brankovic. 2010. Effect of fermentation on antioxidant properties of some cereals and pseudo cereals. Food Chemistry 119: 957–963. https://doi.org/10.1016/j.foodchem.2009.07.049.
Edwards, C.H., M. Rossi, C.P. Corpe, P.J. Butterworth, and P.R. Ellis. 2016. The role of sugars and sweeteners in food, diet and health: Alternatives for the future. Trends in Food Science Technology 56: 158–166. https://doi.org/10.1016/j.tifs.2016.07.008.
Ermiş, E., M. Özkan, and N. Coşar. 2019. Investigation of usability of sugar beet extracts obtained by various processes in lemonade product. Journal of Istanbul Sabahattin Zaim University Natural Sciences Institute 1 (1): 7–10.
Fels, L., F. Jakob, R.F. Vogel, and D. Wefers. 2018. Structural characterization of the exopolysaccharides from water kefir. Carbohydrate Polymers 189: 296–303. https://doi.org/10.1016/j.carbpol.2018.02.037.
Gardner, P.T., T.A.C. White, D.B. McPhail, and G.G. Duthie. 2000. The relative contributions of vitamin C, carotenoids and phenolics to the antioxidant potential of fruit juices. Food Chemistry 68 (4): 471–474. https://doi.org/10.1016/S0308-8146(99)00225-3.
Girones-Vilaplana, A., P. Valentao, D.A. Moreno, F. Ferreres, C. Garcia-Viguera, and P.B. Andrade. 2012. New beverages of lemon juice enriched with the exotic berries maqui, açai and balckthorn: Bioactive components and in vitro biological properties. Journal of Agricultural and Food Chemistry 60: 6571–6580. https://doi.org/10.1021/jf300873k.
Gonzalez-Molina, E., D.A. Moreno, and C. Garcia-Viguera. 2009. A new drink rich in healthy bioactives combining lemon and pomegranate juices. Food Chemistry 115: 1364–1372. https://doi.org/10.1016/j.foodchem.2009.01.056.
Guimaraes-Tavares, P.P.L., E.A. dos Anjos, R.Q. Nascimento, L.F. da Silva Cruz, P.V.F. Lemos, J.I. Druzian, T.T.B. de Oliveira, R.B. de Andrade, A.L. da Costa Souza, K.T. Magalhaes-Guedes, and M.E. de Oliveira Mamede. 2021. Chemical, microbiological and sensory viability of low-calorie, dairy-free kefir beverages from tropical mixed fruit juices. Cyta-Journal of Food 19 (1): 457–464. https://doi.org/10.1080/19476337.2021.1906753.
Gunenc, A., M.H. Yeung, C. Lavergne, J. Bertinato, and F. Hosseinian. 2017. Enhancements of antioxidant activity and mineral solubility of germinated wrinkled lentils during fermentation in kefir. Journal of Functional Foods 32: 72–79. https://doi.org/10.1016/j.jff.2017.02.016.
Hakim, I.A., U.M. Weisgerber, R.B. Harris, D. Balentine, C.A.J. van-Mierlo, and I. Paetau-Robinson. 2000. Preparation, composition and consumption patterns of tea-based beverages in Arizona. Nutrition Research 20: 1715–1724.
Hashemi, S.M.B., A.M. Khaneghah, F.J. Barba, Z. Nemati, S.S. Shokofti, and F. Alizadeh. 2017. Fermented sweet lemon juice (Citrus limetta) using Lactobacillus plantarum LS5: Chemical composition, antioxidant and antibacterial activities. Journal of Functional Foods 38: 409–414. https://doi.org/10.1016/j.jff.2017.09.040.
Huang, D., B. Ou, and R.L. Prior. 2005. The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry 53 (6): 1841–1856. https://doi.org/10.1021/jf030723c.
Klimczak, I., M. Malecka, M. Szlachta, and A. Gliszczynska-Swiglo. 2007. Effect of storage on the content of polyphenols, vitamin C and the antioxidant activity of orange juices. Journal of Food Composition and Analysis 20 (3–4): 313–322. https://doi.org/10.1016/j.jfca.2006.02.012.
Laureys, D., and L. de Vuyst. 2014. Microbial species diversity, community dynamics and metabolite kinetics of water kefir fermentation. Applied Environmental Microbiology 80 (8): 2564–2572. https://doi.org/10.1128/AEM.03978-13.
Laureys, D., M. Aerts, P. Vandamme, and L. de Vuyst. 2018. Oxygen and diverse nutrients influence the water kefir fermentation process. Food Microbiology 73: 351–361.
Lee, S.C., W.J. Chang, K.T. Lu, D. Lo, and M.C. Wu. 2013. Antioxidant capacity and hepatoprotective effect on ethanol-injured liver cell of lemon juice concentrates and its comparison with commercial Japanese apricot juice concentrates. Research Journal of Pharmaceutical Sciences 2 (2): 7–14.
Liu, J.R., and C.W. Lin. 2000. Production of kefir from soymilk with or without added glucose, lactose, or sucrose. Journal of Food Science 65 (4): 716–719. https://doi.org/10.1111/j.1365-2621.2000.tb16078.x.
Liu, R., and F. Shen. 2008. Impacts of main factors on bioethanol fermentation from stalk juice of sweet sorghum by immobilized Saccharomyces cerevisiae (CICCL 1308). Bioresource Technology 99 (4): 847–885. https://doi.org/10.1016/j.biortech.2007.01.009.
Liu, J., Y. Lin, M. Chen, L. Chen, and C. Lin. 2005. Antioxidative activities of Kefir. Asian. Australian. Journal of Animal Science 18 (4): 567–573. https://doi.org/10.5713/ajas.2005.567.
Monajjemi, M., L.A. NurulAminin, A. Ilkhani, and F. Mollaamin. 2012. Nano study of antioxidant activities of fermented soy whey prepared with lactic acid bacteria and kefir. African Journal of Microbiology Research 6 (2): 426–430. https://doi.org/10.5897/AJMR11.1249.
Moufida, S., and B. Marzouk. 2003. Biochemical characterization of blood orange, sweet orange, lemon, bergamot and bitter orange. Phytochemistry 62 (8): 1283–1289. https://doi.org/10.1016/S0031-9422(02)00631-3.
Nordic Committee on Food Analysis—NMKL. 2007. Trace elements - As, Cd, Hg, Pb and other elements: determination by ICP-MS after pressure digestion. Oslo: NMKL.
Ozcelik, F., E. Akan, and O. Kinik. 2021. Use of cornelian cherry, hawthorn, red plum, roseship and pomegranate juices in the production of water kefir beverages. Food Bioscience 42: 101219. https://doi.org/10.1016/j.fbio.2021.101219.
Puerari, C., K.T. Magalhaes, and R.F. Schwan. 2012. New cocoa pulp-based kefir beverages: Microbiological, chemical 591 composition and sensory analysis. Food Research International 48 (2): 634–640. https://doi.org/10.1016/j.foodres.2012.06.005.
Randazzo, W., O. Corona, R. Guarcello, N. Francesca, M.A. German, H. Erten, G. Moschetti, and L. Settanni. 2016. Development of new non-dairy beverages from Mediterranean fruit juices fermented with water kefir microorganisms. Food Microbiology 54: 40–51. https://doi.org/10.1016/j.fm.2015.10.018.
Rodriguez, H., J. Curiel, J. Landete, et al. 2009. Food phenolics and lactic acid bacteria. International Journal of Food Microbiology 132: 79–90. https://doi.org/10.1016/j.ijfoodmicro.2009.03.025.
Sabokbar, N., F. Khodaiyan, and M. Moosavi-Nasab. 2015. Optimization of processing conditions to improve antioxidant activities of apple juice and whey based novel beverage fermented by kefir grains. Journal of Food Science and Technology 52 (6): 3422–3432. https://doi.org/10.1007/s13197-014-1397-4.
Saraiva, A., C. Carrascosa, F. Ramos, D. Raheem, and A. Raposo. 2022. Agave syrup: Chemical analysis and nutritional profile, applications in the food industry and health impacts. International Journal of Environmental Research and Public Health 19: 7022. https://doi.org/10.3390/ijerph19127022.
Sindhu, R., and B.S. Khatkar. 2018. Effects of chemical treatments on storage stability of lemon (Citrus limon) juice. International Advanced Research Journal in Science Engineering and Technology 5 (2): 76–79.
Singleton, V.L., and J.A. Rossi. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16: 144–158.
Surja, L.L., B. Dwiloka, and H. Rizqiati. 2019. Effect of high fructose syrup (HFS) addition on chemical and organoleptic properties of green coconut water kefir. Journal of Applied Food Technology 6 (1): 3–8.
Tamer, C.E., F.Z. Yekeler, Ö.U. Çopur, B. İncedayı, and S. Suna. 2017. A study of fortification of lemonade with herbal extracts. Food Science and Technology 37 (1): 45–51. https://doi.org/10.1590/1678-457X.06016.
Tiencheu, B., D.N. Nji, A.U. Achidi, A.C. Egbe, N.E. Tenyang, F.T. Ngongang, F.T. Djikeng, and B.T. Fossi. 2021. Nutritional, sensory, physico-chemical, phytochemical, microbiological and shelf-life studies of natural fruit juice formulated from orange (Citrus sinensis), lemon (Citrus limon), honey and ginger (Zingiber officinale). Heliyon 7: e07177. https://doi.org/10.1016/j.heliyon.2021.e07177.
Uçan, F., E. Ağçam, and A. Akyıldız. 2014. Natural turbid lemon juice a research on production. Food 39 (1): 25–32.
Voung, T., L. Martin, and C. Matar. 2006. Antioxidant activity of fermented berry juices and their effects on nitric oxide and tumor necrosis factor-alpha production in macrophages 264.7 gamma NO (-) cell line. Journal of Food Biochemistry 30: 249–268. https://doi.org/10.1111/j.1745-4514.2006.00054.x.
Waldherr, F.W., V.M. Doll, D. Meibner, and R.F. dan Vogel. 2010. Identification and characterization of a glucan-producing enzyme from Lactobacillus hilgardii TMW 1.828 involved in granule formation of water kefir. Journal of Food Microbiology 27: 672–678. https://doi.org/10.1016/j.fm.2010.03.013.
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Gülhan, A. Usability of Carbon Sources as Sucrose, Honey and Agave Syrup in Fermentation of Lemonade with Water Kefir Grains. Sugar Tech 25, 1542–1556 (2023). https://doi.org/10.1007/s12355-023-01301-z
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DOI: https://doi.org/10.1007/s12355-023-01301-z