Skip to main content
SpringerLink
Log in

Enhancement of Integrated Sugarcane Trash Managements by Co-inoculation of Cellulolytic Microorganisms for Sustaining Soil Fertility

  • Research Article
  • Published:
Sugar Tech Aims and scope Submit manuscript

Abstract

In this study, a cellulolytic bacterium Bacillus subtilis KKU-KK-3-1 and a fungus Aspergillus sydowii KKU-KK-2-1 were used to decompose sugarcane leaves using a litter bag technique. These microorganisms were investigated for their ability to produce plant growth promoting agents and cellulolytic enzymes. The results showed that they could produce ammonia, which can be a nitrogen source for plant growth, and solubilize inorganic phosphate. Cellulase and xylanase enzymes from these organisms showed high stability over a wide range of pH and temperature, suggesting their potential for use in the fields. The litter bag experiment was set up during a course of 4 months using a randomized complete block design. The results showed that an inoculation of either one of the organisms or co-inoculation of both provided a better decomposition of sugarcane leaves and enhanced physicochemical properties of soils. The best performance was found in the treatment with a single inoculum of Aspergillus sydowii KKU-KK-2-1, in which the highest leaf weight loss of ~ 80% was obtained. Moreover, an increase in organic matter of ~ 5 times, total N of ~ 3 times and available P of ~ 2.5 times were also achieved. These suggested that both Bacillus subtilis KKU-KK-3-1 and Aspergillus sydowii KKU-KK-2-1 could be used as sugarcane leaf decomposers and resulted in a biofertilizer after the degradation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ahmad, F., I. Ahmad, and M. Khan. 2008. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiology Research 163: 173–181. https://doi.org/10.1016/j.micres.2006.04.001.

    Article  CAS  Google Scholar 

  • Agricultural Land Reform Office [ALRO]. 2020. Annual report in 2020. The agricultural cooperative federation of Thailand. Limited. Bangkok, Thailand. P, 132.

  • Ayyachamy, M., and T.M. Vatsala. 2007. Production and partial characterization of cellulase free xylanase by Bacillus subtilis C 01 using agriresidues and its application in biobleaching of nonwoody plant pulps. Letters in Applied Microbiology 45 (5): 467–472. https://doi.org/10.1111/j.1472-765x.2007.02223.x.

    Article  CAS  PubMed  Google Scholar 

  • Baron, N.C., N.T.A. Costa, D.A. Mochi, and E.C. Rigobelo. 2018. First report of Aspergillus sydowii and Aspergillus brasiliensis as phosphorus solubilizers in maize. Annals of Microbiology 68: 863–870. https://doi.org/10.1007/s13213-018-1392-5.

    Article  CAS  Google Scholar 

  • Biederbeck, V., C.A. Campbell, K.E. Bowren, M. Schnitzer, and R.N. McIver. 1980. Effect of burning cereal straw on soil properties and effects of organic inputs applied to rice. A contrasting grain yields in Saskatchewan. Soil Science Society of America Journal 44: 103–111. https://doi.org/10.2136/sssaj1980.03615995004400010022x.

    Article  CAS  Google Scholar 

  • Brady, N.C.; and R.R. Weil. 2008. The nature and properties of soils. 14ed. Pearson-Prentice Hall, Upper Saddle River, NJ, USA.

  • Cerri, C.C., M.V. Galdos, S.M.F. Maia, M. Bernoux, B.J. Feigl, D. Powlson, and C.E.P. Cerri. 2011. Effect of sugarcane harvesting systems on soil carbon stocks in Brazil: An examination of existing data. European Journal of Soil Science 62: 23–28. https://doi.org/10.1111/j.1365-2389.2010.01315.x.

    Article  CAS  Google Scholar 

  • Choudhary, M., P.C. Sharma, H.S. Jat, V. Nehra, A.J. McDonald, and N. Garg. 2016. Crop residue degradation by fungi isolated from conservation agriculture fields under rice–wheat system of North–West India. International Journal of Recycling of Organic Waste in Agriculture 5 (4): 349–360. https://doi.org/10.1007/s40093-016-0145-3.

    Article  Google Scholar 

  • Deng, Z., A. Xia, Q. Liao, X. Zhu, Y. Huang, and Q. Fu. 2019. Laccase pretreatment of wheat straw: Effects of the physicochemical characteristics and the kinetics of enzymatic hydrolysis. Biotechnology for Biofuels 12: 159. https://doi.org/10.1186/s13068-019-1499-3.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fortes, C., P.C.O. Trivelin, and A.C. Vitti. 2012. Long-term decomposition of sugarcane harvest residues in São Paulo state. Brazil. Biomass and Bioenergy 42: 189–198. https://doi.org/10.1016/j.biombioe.2012.03.011.

    Article  CAS  Google Scholar 

  • Galdos, M.V., C.C. Cerri, C.E.P. Cerri, K. Paustian, and R. Antwerpen. 2009. Simulation of soil carbon dynamics under sugarcane with the CENTURY model. Soil Science Society of America Journal 73: 802–811. https://doi.org/10.2136/sssaj2007.0285.

    Article  CAS  Google Scholar 

  • Hashem, A., B. Tabassum, and E.F. AbdAllah. 2019. Bacillus subtilis: A plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi Journal of Biological Sciences 26 (6): 1291–1297. https://doi.org/10.1016/j.sjbs.2019.05.004.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hemwong, S., G. Cadisch, B. Toomsan, V. Limpinuntana, P. Vityakon, and A. Patanothai. 2008. Dynamics of residue decomposition and N2 fixation of grain legumes upon sugarcane stover retention as an alternative to burning. Soil & Tillage Research 99: 84–97. https://doi.org/10.1016/j.still.2008.01.003.

    Article  Google Scholar 

  • Lefroy, R.D.B., W. Chaitep, and G.J. Blair. 1994. Release of sulfur from rice residues under flooded and non-flooded soil conditions. Australian Journal of Agricultural Research 45: 657–667.

    Article  CAS  Google Scholar 

  • Li, X.G., B. Jia, J. Lv, Q. Ma, Y. Kuzyakov, and F. Li. 2017. Nitrogen fertilization decreases the decomposition of soil organic matter and plant residues in planted soils. Soil Biology and Biochemistry 112: 47–55. https://doi.org/10.1016/j.soilbio.2017.04.018.

    Article  CAS  Google Scholar 

  • Maza, M., H.F. Pajot, M.J. Amoroso, and M.G. Yasem. 2014. Post-harvest sugarcane residue degradation by autochthonous fungi. International Biodeterioration & Biodegradation 87: 18–25. https://doi.org/10.1016/j.ibiod.2013.10.020.

    Article  CAS  Google Scholar 

  • Miura, T., A. Niswati, I.G. Swibawa, S. Haryani, H. Gunito, and N. Kaneko. 2013. No tillage and bagasse mulching alter fungal biomass and community structure during decomposition of sugarcane leaf litter in Lampung Province, Sumatra Indonesia. Soil Biology and Biochemistry 58: 27–35. https://doi.org/10.1016/j.soilbio.2012.10.042.

    Article  CAS  Google Scholar 

  • Moteshafi, H., M. Hashemi, S.M. Mousavi, and M. Mousivand. 2016. Characterization of produced xylanase by Bacillus subtilis D3d newly isolated from apricot phyllosphere and its potential in pre-digestion of BSG. Journal of Industrial and Engineering Chemistry 37: 251–260. https://doi.org/10.1016/j.jiec.2016.03.036.

    Article  CAS  Google Scholar 

  • Nelson, N. 1944. A photometric adaptation of Somogyi method for the determination of glucose. Journal of Biological Chemistry 153: 375–380.

    Article  CAS  Google Scholar 

  • Olsen, S.R., and L.E. Sommers. 1982. Phosphorus. In: Page, A.L., R.H. Miller, and R.K. Dennis. (Eds) Methods of Soil Analysis. Madison: American Society of Agronomy, USA, p: 403–430.

  • Pandya, N.D., P.V. Desai, H.P. Jadhav, and R.Z. Sayyed. 2018. Plant growth promoting potential of Aspergillus sp. NPF7, isolated from wheat rhizosphere in South Gujarat India. Environmental Sustainability 1: 245–252. https://doi.org/10.1007/s42398-018-0025-z.

    Article  Google Scholar 

  • Phukongchai, W., and W. Kaewpradit. 2018. Impact of sugarcane trash managements on decomposition and N release. Khon Kaen Agriculture Journal 46 (1): 25–29.

    Google Scholar 

  • Pikovskaya, R.I. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiología 17: 362–370.

    CAS  Google Scholar 

  • Pitakpakorn, W., K. Sinma, S. Amkha, C. Thongjoo, C. Khongsud, T. Klumchaunt, P. Katpiyarat, T. Kulkaranlert, A. Isuwan, and S. Bootpetch. 2016. Nitrogen fertilizer influenced on decomposition of sugarcane leaf residues under aerobic and submerged conditions in kamphaengsaen soil series. Songklanakarin Journal of Plant Science 3 (2): 28–38.

    Google Scholar 

  • Raj, A., S. Kumar, S.K. Singh, and J. Prakash. 2018. Production and purification of xylanase from alkaliphilic Bacillus licheniformis and its pretreatment of eucalyptus kraft pulp. Biocatalysis and Agricultural Biotechnology 15: 199–209. https://doi.org/10.1016/j.bcab.2018.06.018.

    Article  Google Scholar 

  • Saeid, A., E. Prochownik, and J. Dobrowolska-Iwanek. 2018. Phosphorus Solubilization by Bacillus Species. Molecules 23 (11): 2897. https://doi.org/10.3390/molecules23112897.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shruti, S.A., and M.D. Kumar. 2015. Lignocellulose biomass degradation by microbial consortium isolated from harvested rice field. International Journal of Current Microbiology and Applied Sciences 4: 274–280.

    Google Scholar 

  • Singh, G., N. Capalash, and P. Sharma. 2009. Performance of an alkalophilic and halo tolerant laccase from c-proteobacterium JB in the presence of industrial pollutants. Journal of General and Applied Microbiology 55: 283–289. https://doi.org/10.2323/jgam.55.283.

    Article  CAS  PubMed  Google Scholar 

  • Swain, M.R., K. Laxminarayana, and R.C. Ray. 2012. Phosphorus solubilization by Thermotolerant Bacillus subtilis Isolated from Cow dung microflora. Agricultural Research 1 (3): 273–279. https://doi.org/10.1007/s40003-012-0022-x.

    Article  CAS  Google Scholar 

  • Thongjoo, C., S. Miyagawa, and N. Kawakubo. 2006. Soil productivity after decomposition of waste materials under different soil moisture and temperature. Plant Science 9: 106–114. https://doi.org/10.1626/pps.9.106.

    Article  Google Scholar 

  • Vilela, L.d.F., de Araujo, V.P.G., Paredes R.d.S., Bon E.P.d.S., Torres F.A.G., Neves B.C., and Eleutherio E.C.A.. 2015. Enhanced xylose fermentation and ethanol production by engineered Saccharomyces cerevisiae strain. AMB Express 5: 16. https://doi.org/10.1186/s13568-015-0102-y.

  • Vu, V., C. Farkas, O. Riyad, E. Bujna, A. Kilin, G. Sipiczki, M. Sharma, Z. Usmani, V.K. Gupta, and Q.D. Nguyen. 2022. Enhancement of the enzymatic hydrolysis efficiency of wheat bran using the Bacillus strains and their consortium. Bioresource Technology 343: 126092. https://doi.org/10.1016/j.biortech.2021.126092.

    Article  CAS  PubMed  Google Scholar 

  • Wu, H., X. Cheng, Y. Zhu, W. Zeng, G. Chen, and Z. Liang. 2018. Purification and characterization of a cellulase-free, thermostable endo-xylanase from Streptomyces griseorubens LH-3 and its use in biobleaching on eucalyptus kraft pulp. Journal of Bioscience and Bioengineering 125: 46–51. https://doi.org/10.1016/j.jbiosc.2017.08.006.

    Article  CAS  PubMed  Google Scholar 

  • Yadav, J., J.P. Verma, and K.N. Tiwari. 2011. Plant growth promoting activities of fungi and their effect on chickpea plant growth. Asian Journal of Biological Sciences 4 (3): 291–299. https://doi.org/10.3923/ajbs.2011.291.299.

    Article  Google Scholar 

  • Ying, W.J., Z.J. Shi, H.Y. Yang, G.F. Xu, Z.F. Zhang, and J. Yang. 2018. Effect of alkaline lignin modification on cellulase-lignin interactions and enzymatic saccharification yield. Biotechnology for Biofuels 11: 13. https://doi.org/10.1186/s13068-019-1499-3.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was funded by the Centre of Excellence on Biodiversity (BDC), Office of Higher Education Commission with project code BDC-PG1-163002, and Salt-tolerant Rice Research Group Khon Kaen University, Thailand.

Author information

Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Wasan Seemakram, Saranya Khaekhum, Jindarat Ekprasert & Sophon Boonlue

  2. Faculty of Allied Health, Nakhon Ratchasima College, Nakhon Ratchasima, 30000, Thailand

    Thanapat Suebrasri

Authors
  1. Wasan Seemakram
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thanapat Suebrasri
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Saranya Khaekhum
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jindarat Ekprasert
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sophon Boonlue
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sophon Boonlue.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Seemakram, W., Suebrasri, T., Khaekhum, S. et al. Enhancement of Integrated Sugarcane Trash Managements by Co-inoculation of Cellulolytic Microorganisms for Sustaining Soil Fertility. Sugar Tech 25, 925–937 (2023). https://doi.org/10.1007/s12355-023-01250-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12355-023-01250-7

Keywords

Search

Navigation