Abstract
The beet-processing sugar industry is facing a severe crisis in some countries due to high production costs. This situation also negatively affects the beet producers. To increase the profit of beet-processing sugar plants, it is considered that the evaluation of by-products such as molasses, beet-pulp, and carbonation cake to obtain valuable organic acids could be commercially available. In this study, gluconic acid production by fermentation with A. niger has been investigated by using by-products obtained from beet-processing sugar plant. The experimental conditions have been optimized for gluconic acid production by submerged, semi solid-state, and solid-state fermentation routes by using Response Surface Methodology (RSM). Gluconic acid production efficiencies under the optimized conditions for submerged, semi solid-state, and solid-state fermentations were calculated as 0.258, 0.354, and 0.262 g gluconic acid per g of absolute solid substrate, respectively. It has been determined that citric and oxalic acid together with gluconic acid could be produced at the significant levels for all three fermentation routes. It has been observed that obtaining oxalic and citric acid along with gluconic acid using A. niger depend strongly on pH. Gluconic acid was produced at near-neutral pH, whereas oxalic (pH ~ 4–4.5) and citric (pH < 2) acids were obtained at lower pH. In conclusion, in-situ production of gluconic and important organic acids can be realized by using beet-processing sugar plant by-products and some waste energy with simple fermentation techniques. Under the present circumstances, this approach is the most convenient way to survive the crisis that uses appropriate fungal/bacterial cultures.
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References
Ahmed, K., E.E. Valeem, and Q. Ul Haq. 2014. Optimal conditions for the production of industrial enzymes By Aspergillus Niger using agricultural wastes as source of carbon. FUUAST Journal of Biology 4: 205–211.
Ahmed, A.S., S.S. Farag, I.A. Hassan, and H.W. Botros. 2015. Production of gluconic acid by using some irradiated microorganisms. Journal of Radiation Research and Applied Sciences 8: 374–380. https://doi.org/10.1016/j.jrras.2015.02.006.
Ajala, E.O., M.A. Ajala, D.S. Ogunniyi, and M.O. Sunmonu. 2017. Kinetics of gluconic acid production and cell growth in a batch bioreactor by Aspergillus niger using breadfruit hydrolysate. Journal of Food Process Engineering 40: e12461. https://doi.org/10.1111/jfpe.12461.
Amaniampong, P.N., K. Li, X. Jia, B. Wang, A. Borgna, and Y. Yang. 2014. Titania-supported gold nanoparticles as efficient catalysts for the oxidation of cellobiose to organic acids in aqueous medium. ChemCatChem 6: 2105–2114. https://doi.org/10.1002/cctc.201402096.
Amaniampong, P.N., A.Y. Booshehri, X. Jia, Y. Dai, B. Wang, S.H. Mushrif, A. Borgna, and Y. Yang. 2015. High-temperature reduction improves the activity of rutile TiO2 nanowires-supported gold-copper bimetallic nanoparticles for cellobiose to gluconic acid conversion. Applied Catalysis a: General 505: 16–27. https://doi.org/10.1016/j.apcata.2015.07.027.
An, D., A. Ye, W. Deng, Q. Zhang, and Y. Wang. 2012. Selective conversion of cellobiose and cellulose into gluconic acid in water in the presence of oxygen, catalyzed by polyoxometalate-supported gold nanoparticles. Chemistry - A European Journal 18: 2938–2947. https://doi.org/10.1002/chem.201103262.
Anastassiadis, S., and I. Morgunov. 2008. Gluconic Acid Production. Recent Patents on Biotechnology 1: 167–180. https://doi.org/10.2174/187220807780809472.
Bao, J., K. Furumoto, K. Fukunaga, and K. Nakao. 2001. A kinetic study on air oxidation of glucose catalyzed by immobilized glucose oxidase for production of calcium gluconate. Biochemical Engineering Journal 8: 91–102. https://doi.org/10.1016/S1369-703X(00)00140-6.
Biella, S., L. Prati, and M. Rossi. 2003. Selectivity control in the oxidation of phenylethane-1,2-diol with gold catalyst. Inorganica Chimica Acta 349: 253–257. https://doi.org/10.1016/S0020-1693(03)00040-9.
Bin, D., H. Wang, J. Li, H. Wang, Z. Yin, J. Kang, B. He, and Z. Li. 2014. Controllable oxidation of glucose to gluconic acid and glucaric acid using an electrocatalytic reactor. Electrochimica Acta 130: 170–178. https://doi.org/10.1016/j.electacta.2014.02.128.
Cameselle, C., J.T. Bohlmann, M.J. Núñez, and J.M. Lema. 1998. Oxalic acid production by Aspergillus niger. Part I: Influence of sucrose and milk whey as carbon source. Bioprocess Engineering 19: 247–252. https://doi.org/10.1007/s004490050515.
Chuppa-Tostain, G., J. Hoarau, M. Watson, L. Adelard, A.S.C. Sing, Y. Caro, I. Grondin, I. Bourven, J.-M. Francois, E. Girbal-Neuhauser, and T. Petit. 2018. Production of Aspergillus niger biomass on sugarcane distillery wastewater: Physiological aspects and potential for biodiesel production. Fungal Biology and Biotechnology 5: 1–12. https://doi.org/10.1186/s40694-018-0045-6.
Concha, O., and J., and M. E. Zúñiga Hansen. 2012. Enzymatic depolymerization of sugar beet pulp: Production and characterization of pectin and pectic-oligosaccharides as a potential source for functional carbohydrates. Chemical Engineering Journal 192: 29–36. https://doi.org/10.1016/j.cej.2012.03.085.
Crognale, S., M. Petruccioli, M. Fenice, and F. Federici. 2008. Fed-batch gluconic acid production from Penicillium variabile P16 under different feeding strategies. Enzyme and Microbial Technology 42: 445–449. https://doi.org/10.1016/j.enzmictec.2008.01.002.
Curie J., J. Kane, and A. Finlay 1931. Process for producing gluconic acid by fungi, US Patent 1,893,819. United State.
Gunst, R.F., R.H. Myers, and D.C. Montgomery. 1996. Response surface methodology: process and product optimization using designed experiments. Technometrics 38: 285–286. https://doi.org/10.2307/1270613.
Hayashi, S., and S. Nakamura. 1981. Multiple forms of glucose oxidase with different carbohydrate compositions. BBA - Enzymology 657: 40–51. https://doi.org/10.1016/0005-2744(81)90128-5.
Hisashi S., S. Ohnaka, and S. Fukuda. 1989. Process for producing gluconic acid, US 4,843,173. United State.
Hustede, H., H.-J. Haberstroh, and E. Schinzig. 1989. Gluconic acid. Ullmann’s encyclopedia of industrial chemistry 15. VCH Weinheim: 211–216.
Ikeda, Y., E.Y. Park, and N. Okuda. 2006. Bioconversion of waste office paper to gluconic acid in a turbine blade reactor by the filamentous fungus Aspergillus niger. Bioresource Technology 97: 1030–1035. https://doi.org/10.1016/j.biortech.2005.04.040.
Kornecki, J.F., D. Carballares, P.W. Tardioli, R.C. Rodrigues, Á. Berenguer-Murcia, A.R. Alcántara, and R. Fernandez-Lafuente. 2020. Enzyme production of d-gluconic acid and glucose oxidase: Successful tales of cascade reactions. Catalysis Science and Technology 10: 5740–5771. https://doi.org/10.1039/d0cy00819b.
Li, S., S. Xu, S. Liu, C. Yang, and Q. Lu. 2004. Fast pyrolysis of biomass in free-fall reactor for hydrogen-rich gas. Fuel Processing Technology 85: 1201–1211. https://doi.org/10.1016/j.fuproc.2003.11.043.
Lim, H.Y., and A.V. Dolzhenko. 2021. Gluconic acid aqueous solution: A bio-based catalytic medium for organic synthesis. Sustainable Chemistry and Pharmacy 21: 100443. https://doi.org/10.1016/j.scp.2021.100443.
Matsui, T., K. Tooyama, and S. Sato. 2013. Simultaneous saccharification of corn starch in gluconic acid production by Aspergillus niger immobilized on nonwoven fabric in a pressurized reactor. Journal of Microbial and Biochemical Technology 5: 88–91. https://doi.org/10.4172/1948-5948.1000106.
McCready, R.M. 1966. Polysaccharides of sugar beet pulp, a review of their chemistry. Journal of Sugarbeet Research 14: 260–270. https://doi.org/10.5274/jsbr.14.3.260.
Michel, F., J.-F. Thibault, J.-L. Barry, and R. de Baynast. 1988. Preparation and characterisation of dietary fibre from sugar beet pulp. Journal of the Science of Food and Agriculture 42: 77–85. https://doi.org/10.1002/jsfa.2740420109.
Morawa, E.K., M.F.R. Pereira, and J.L. Figueiredo. 2016. One-pot oxidation of cellobiose to gluconic acid. Unprecedented high selectivity on bifunctional gold catalysts over mesoporous carbon by integrated texture and surface chemistry optimization. Applied Catalysis b: Environmental 184: 381–396. https://doi.org/10.1016/j.apcatb.2015.10.011.
Mukhopadhyay, R., S. Chatterjee, B.P. Chatterjee, P.C. Banerjee, and A.K. Guha. 2005. Production of gluconic acid from whey by free and immobilized Aspergillus niger. International Dairy Journal 15: 299–303. https://doi.org/10.1016/j.idairyj.2004.07.010.
Nigam, P., and M. Vogel. 1991. Bioconversion of sugar industry by-products-molasses and sugar beet pulp for single cell protein production by yeasts. Biomass and Bioenergy 6: 339–345. https://doi.org/10.1016/0961-9534(91)90014-4.
Onda, A., T. Ochi, and K. Yanagisawa. 2011. New direct production of gluconic acid from polysaccharides using a bifunctional catalyst in hot water. Catalysis Communications 12: 421–425. https://doi.org/10.1016/j.catcom.2010.10.023.
Petruccioli, M., P. Piccioni, M. Fenice, and F. Federici. 1994. Glucose oxidase, catalase and gluconic acid production by immobilized mycelium of Penicillum variabile P16. Biotechnology Letters 16: 939–942. https://doi.org/10.1007/BF00128629.
Ramachandran, S., P. Fontanille, A. Pandey, and C. Larroche. 2006. Gluconic acid: Properties, applications and microbial production. Food Technology and Biotechnology 44: 185–195.
Roehr, M., C.P. Kubicek, and J. Komínek. 2008. Gluconic Acid. In Biotechnology: Second, Completely Revised Edition, ed. Roehr M. Rehm H.J., Reed G., 6–12:347–362. Weinheim: Verlag Chemical. doi: https://doi.org/10.1002/9783527620999.ch10f.
Roukas, T. 2000. Citric and gluconic acid production from fig by Aspergillus niger using solid-state fermentation. Journal of Industrial Microbiology and Biotechnology 25: 298–304. https://doi.org/10.1038/sj.jim.7000101.
Roukas, T., and L. Harvey. 1988. The effect of pH on production of citric and gluconic acid from beet molasses using continuous culture. Biotechnology Letters 10: 289–294. https://doi.org/10.1007/BF01024422.
Roukas, T., and M. Liakopoulou-Kyriakides. 2002. Optimization study for the production of citric and gluconic acid from fig water extract by Aspergillus niger in surface fermentation. Food Biotechnology 16: 17–28. https://doi.org/10.1081/FBT-120004199.
Sahasrabudhe, N.A., and N.V. Sankpal. 2001. Production of organic acids and metabolites of fungi for food industry. Applied Mycology and Biotechnology 1: 387–425. https://doi.org/10.1016/S1874-5334(01)80016-2.
Sharma, A., V. Vivekanand, and R.P. Singh. 2008. Solid-state fermentation for gluconic acid production from sugarcane molasses by Aspergillus niger ARNU-4 employing tea waste as the novel solid support. Bioresource Technology 99: 3444–3450. https://doi.org/10.1016/j.biortech.2007.08.006.
Singh, N.B. 1976. Effect of gluconates on the hydration of cement. Cement and Concrete Research 6: 455–460. https://doi.org/10.1016/0008-8846(76)90074-0.
Singh, O.V., N. Kapur, and R.P. Singh. 2005. Evaluation of agro-food byproducts for gluconic acid production by Aspergillus niger ORS-4.410. World Journal of Microbiology and Biotechnology 21: 519–524. https://doi.org/10.1007/s11274-004-2395-x.
Singh, O.V., and R.P. Singh. 2006. Bioconversion of grape must into modulated gluconic acid production by Aspergillus niger ORS-4.410. Journal of Applied Microbiology 100: 1114–1122. https://doi.org/10.1111/j.1365-2672.2006.02870.x.
Singh, O.V., B.M.J. Pereira, and R.P. Singh. 1999. Isolation and characterization of a potent fungal strain Aspergillus niger ORS-4 for gluconic acid production. Journal of Scientific and Industrial Research 58: 594–600.
Singh, O.V., and R.P. Sing. 2002. Utilization of agro-food by-products for gluconic acid production by Aspergillus niger ORS-4 under surface culture cultivation. Journal of Scientific and Industrial Research 61: 356–360.
Stubbs, J.J., L.B. Lockwood, E.T. Roe, B. Tabenkin, and G.E. Ward. 1940. Ketogluconic acids from glucose: Bacterial production. Industrial and Engineering Chemistry 32: 1626–1631. https://doi.org/10.1021/ie50372a024.
Sun, R., and S. Hughes. 1998. Extraction and physico-chemical characterization of pectins from sugar beet pulp. Polymer Journal 30: 671–677. https://doi.org/10.1295/polymj.30.671.
Wells, P.A., A.J. Moyer, J.J. Stubbs, H.T. Herrick, and O.E. May. 1937. Gluconic acid production: Effect of pressure, air flow, and agitation on gluconic acid production by submerged mold growths. Industrial and Engineering Chemistry 29: 653–656. https://doi.org/10.1021/ie50330a012.
Werpy, T., and G. Petersen. 2004. Top value added chemicals from biomass: Volume i - results of screening for potential candidates from sugars and synthesis gas. Office of Scientific and Technical Information (OSTI). https://doi.org/10.2172/15008859.
Yan, W., D. Zhang, Y. Sun, Z. Zhou, Y. Du, Y. Du, Y. Li, et al. 2020. Structural sensitivity of heterogeneous catalysts for sustainable chemical synthesis of gluconic acid from glucose. Chinese Journal of Catalysis 41: 1320–1336. https://doi.org/10.1016/S1872-2067(20)63590-2.
Zhang, H., J. Zhang, and J. Bao. 2016. High titer gluconic acid fermentation by Aspergillus niger from dry dilute acid pretreated corn stover without detoxification. Bioresource Technology 203: 211–219. https://doi.org/10.1016/j.biortech.2015.12.042.
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This work was supported by the Scientific Research Projects Coordination Unit of Fırat University (FÜBAP) under the project number MF.16.17. Also, scholarship support was provided by The Scientific and Technological Research Council of Turkey (TÜBİTAK) with the 2210-C Program.
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Kelleci, K., Altundoğan, H.S. & Tanyıldızı, M.Ş. Valorization of Beet-Processing Sugar Factory by-Products for in-situ Gluconic Acid Production by using Aspergillus Niger Fermentation. Sugar Tech 25, 410–421 (2023). https://doi.org/10.1007/s12355-022-01206-3
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DOI: https://doi.org/10.1007/s12355-022-01206-3