Abstract
Acidovorax avenae causes red stripe disease, which is characterised by red stripe and top rot symptoms in sugarcane (Saccharum spp. hybrids). The incidence of one or both these symptoms in the sugarcane fields was up to 30% in the Chiapas and Veracruz states of Mexico between 2015 and 2016. This study aimed to identify the causative agent of these symptoms and to determine the distribution and incidence of the disease in sugarcane-producing agroecological regions. Eleven bacterial isolates with phenotypic characteristics similar to those of A. avenae were isolated from the symptomatic tissue. Phylogenetic reconstruction based on the 16S rDNA sequence identified these 11 isolates as A. avenae with 99% identity. A. avenae infection was identified in three of the seven agroecological regions of Mexico (Cordoba-Gulf, Papaloapan-Gulf, and Southern region) with incidences ranging between 1 and 55%. The highest incidence of A. avenae infection was in the COLMEX 94-8 variety in the Cordoba-Gulf region (55%), whereas the lowest (1%) incidence was in the CP 72-2086 variety in the Papaloapan-Gulf region. The inoculation of these 11 strains resulted in the development of necrosis and wilting of the youngest leaves, dead of inoculated leave, and stem rot symptoms in the susceptible variety CP 72-2086. This is the first study to report the distribution and incidence of A. avenae in sugarcane plantations of Mexico.
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Acknowledgements
The authors thank the SAGARPA-CONACYT Sectorial Fund for the funding received for this research through the project ‘Diagnosis and comprehensive management of the bacterial syndrome associated with Xanthomonas spp. and of emerging diseases of sugarcane (Saccharum officinarum) in Mexico (Code 2013-02-230876)’ as well as the Biotechnology and Seed Pathology laboratory of the Postgraduate College, Campus Montecillo, (external project PS 15-1063) for partially funding this research.
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CHJ conducted the experiments, collected the data, and wrote a Doctorate dissertation based on this investigation. SAO conceptualised, designed, and conducted the investigation, as well as reviewed and edited the original manuscript draft. HVSR conceptualised and conducted the investigation, provided inputs for phylogenetic analysis, and partially sponsored the project. EHJ performed statistical analyses. MOG, GVP, GNM, and CDLGA conducted the investigation and edited the manuscript. All authors have read and approved the final manuscript.
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Hernández-Juárez, C., Silva-Rojas, H.V., De León García de Alba, C. et al. Molecular Identification, Incidence, and Distribution of Acidovorax avenae in the Sugarcane-Producing Agroecological Regions of Mexico. Sugar Tech 23, 891–899 (2021). https://doi.org/10.1007/s12355-021-00964-w
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DOI: https://doi.org/10.1007/s12355-021-00964-w