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Molecular Characterization and Promoter Analysis of Novel Sugar Transporter Gene ScERD6 in Sugarcane

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Abstract

Plant sugar transporters are key physiological elements in transporting sucrose and sensing specific sucrose signals. A knockoff of the novel sugar transporter gene (ScERD6) was developed by RACE-PCR from sugarcane, which incorporated an ORF of 1521 bp encoding a protein of 506 amino acids. The isolated ScERD6 gene was measured by 4722 bp DNA and containing 17 exons and 16 introns. It was found that the ScERD6 only had one transcript, and the ScERD6 protein consisted of potentially 12 transmembrane domains, belonging to sugar transporter protein. The promoter sequence was cloned by using genome walking, which included CAAT-boxes and TATA-boxes, as well as specific acting elements such as cis-regulatory elements involved in hormone response, seed development, environmental stresses, ACES, G-box, I-box, L-box, and rbcS-CMA7 light response element, etc. The treatments with 15% PEG and 6 °C stress can induce the expression of ScERD6 in the leaf, root, and stem. But ScERD6 expression in stem was different from that in leaf and root of sugarcane. Real-time PCR analysis uncovered the presence of ScERD6 in roots, stems, leaves, inflorescence, and buds, excessively, and the highest transcript level was observed in inflorescence, and the lowest in roots, while the average was detected in the stem. This finding predicted an important role of this gene in reproductive and floral development.

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Funding

This study was supported in part by National Natural Science Foundation of China (31860403), Guangxi Natural Science Foundation (2017GXNSFBA198023, 2018GXNSFAA294041), Guangxi Funds for Bagui Scholars and Distinguished Experts (2013-3), National Key R&D Program of China (Grant No. 2018YFD1000500), Guangxi Science and Technology Base and Talents Special Project (Grant No. Guike AD17195100), and Project of Guangxi Sugarcane Innovation team of National Modern Agriculture Industry Technology System (gjnytxgxcxtd-03, nycytxgxcxtd-03-01).

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Author notes

  1. Xiao-Rong Miao and Xu-Peng Zeng have contributed equally to this work.

Authors and Affiliations

  1. College of Biology and Pharmacy, Yulin Normal University, Yulin, 537000, China

    Xiao-Rong Miao & Jun-Qi Niu

  2. Agricultural College, Guangxi University, Nanning, 530005, China

    Xiao-Rong Miao, Xu-Peng Zeng, Jun-Qi Niu, Aamir Mahmood & Li-Tao Yang

  3. Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences, Nanning, 530007, China

    Bao-Qing Zhang & Yang-Rui Li

Authors
  1. Xiao-Rong Miao
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  2. Xu-Peng Zeng
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  3. Jun-Qi Niu
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  4. Aamir Mahmood
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  5. Li-Tao Yang
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  6. Bao-Qing Zhang
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  7. Yang-Rui Li
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Correspondence to Jun-Qi Niu, Li-Tao Yang or Yang-Rui Li.

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Miao, XR., Zeng, XP., Niu, JQ. et al. Molecular Characterization and Promoter Analysis of Novel Sugar Transporter Gene ScERD6 in Sugarcane. Sugar Tech 22, 686–696 (2020). https://doi.org/10.1007/s12355-020-00803-4

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