Abstract
To confirm the phytoplasma association in 13 sugarcane white leaf (SCWL) symptomatic samples collected from Puer, China, nested PCR assays with phytoplasma universal primer pairs was performed. A ~ 1240 bp product was obtained from 10 out of 13 symptomatic samples, and 16S rRNA gene sequencing results showed that all sequences of SCWL isolates were 100% identical to each other. BLASTn analysis revealed 100% sequence similarity with the 16S rRNA genes from SCWL phytoplasma strains LC7 and LC9 from Lincang, Yunnan province, China belonging to 16SrXI-B subgroup. Virtual restriction fragment length polymorphism also confirmed that Puer SCWL strains were member of the 16SrXI-B subgroup.
References
Hanboonsong, Y., C. Choosai, S. Panyim, and S. Damak. 2002. Transovarial transmission of sugarcane white leaf phytoplasma in the insect vector Matsumuratettix hiroglyphicus (Matsumura). Insect Molecular Biology 11(1): 97–103.
Hanboonsong, Y., W. Ritthison, C. Choosai, and P. Sirithorn. 2006. Transmission of sugarcane white leaf phytoplasma by Yamatotettix flavovittatus, a new leafhopper vector. Journal of Economic Entomology 99(5): 1531–1537.
Kumarasinghe, N.C., and P. Jones. 2001. Identification of white leaf disease of sugarcane in Sri Lanka. Sugar Tech 3(1): 55–58.
Lee, I., D.E. Gundersenrindal, R.E. Davis, and I.M. Bartoszyk. 1998. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. International Journal of Systematic Bacteriology 48(4): 1153–1169.
Lee, I.M., M. Pastore, M. Vibio, A. Danielli, S. Attathom, R.E. Davis, and A. Bertaccini. 1997. Detection and characterization of a phytoplasma associated with annual blue grass (Poa annua) white leaf disease in southern Italy. European Journal of Plant Pathology 103(3): 251–254.
Li, W.F., H.L. Shan, Y.K. Huang, X.Y. Wang, R.Y. Zhang, Z.M. Luo, and J. Yin. 2014. Occurrence and control strategies of quarantine disease sugarcane white leaf. Sugar Crops of China 3: 66–68.
Li, W.F., X.Y. Wang, Y.K. Huang, K. Shen, H.L. Shan, Z.M. Luo, J. Yin, Y.M. Jia, Z.G. Bai, and R.Y. Zhang. 2013. First report of sugarcane white leaf phytoplasma in Yunnan province, China. Canadian Journal of Plant Pathology 35(3): 407–410.
Ling, K.C. 1962. White leaf disease of sugarcane. Taiwan Sugar 9: 1–5.
Marcone, C. 2002. Phytoplasma diseases of sugarcane. Sugar Tech 4(3–4): 79–85.
Rao, G.P., M. Priya, V. Thorat, and R. Manimekalai. 2017. A century progress of research on phytoplasma diseases in India. Phytopathogenic Mollicutes 7(1): 1–38.
Smart, C.D., B. Schneider, C.L. Blomquist, L.J. Guerra, N.A. Harrison, U. Ahrens, K.-H. Lorenz, E. Seemüller, and B.C. Kirkpatrick. 1996. Phytoplasma-specific PCR primers based on sequences of the 16S-23S rRNA spacer region. Applied and Environmental Microbiology 62(8): 2988–2993.
Thein, M.M., T. Jamjanya, Y. Kobori, and Y. Hanboonsong. 2012. Dispersal of leafhoppers Matsumuratettix hiroglyphicus and Yamatotettix flavovittatus (Homoptera: cicadellidae), vectors of sugarcane white leaf disease. Applied Entomology and Zoology 47(3): 255–262.
Tiwari, A.K., S. Kumar, S. Mall, V. Jadon, and G.P. Rao. 2017. New efficient natural leafhopper vectors of sugarcane grassy shoot phytoplasma in India. Sugar Tech 19(2): 191–197.
Wang, X.Y., W.F. Li, Y.K. Huang, R.Y. Zhang, H.L. Shan, Z.M. Luo, J. Yin, and K. Shen. 2015. Identification of sugarcane white leaf phytoplasma in fields and quaratine sugarcane samples in Yunnan province, China. Sugar Tech 17(1): 85–88.
Win, N.K.K., and H.Y. Jung. 2012. The distribution of phytoplasmas in Myanmar. Journal of Phytopathology 160(3): 139–145.
Woffelman, C. 2004. DNAMAN for windows, version 5.2.10: Lynon Biosoft. Leiden, The Netherlands: Institute of Molecular Plant Sciences, Leiden University.
Wongkaew, P. 2012. Sugarcane white leaf disease characterization, diagnosis development, and control strategies. Functional Plant Science Biotechnology 6(2): 73–84.
Zhang, R.Y., W.F. Li, Y.K. Huang, X.Y. Wang, H.L. Shan, Z.M. Luo, and J. Yin. 2016. Group 16SrXI phytoplasma strains, including subgroup 16SrXI-B and a new subgroup, 16SrXI-D, are associated with sugarcane white leaf. International Journal of Systematic and Evolutionary Microbiology 66(1): 487–491.
Zhao, Y., W. Wei, I.M. Lee, J. Shao, X.B. Suo, and R.E. Davis. 2009. Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). International Journal of Systematic and Evolutionary Microbiology 59(10): 2582–2593.
Acknowledgements
This work was supported by the National Natural Science Foundation of China (31760504), Yunnan Agricultural Foundation Projects [2017FG001(-054)], the Sugar Crop Research System (CARS-170303), Yunling Industry and Technology Leading Talent Training Program “Prevention and Control of Sugarcane Pests” (2018LJRC56), and the Yunnan province Agriculture Research System (YNGZTX-4-92).
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RYZ analyzed the sequences and wrote the manuscript. WFL, XYW, JL, HLS, XYC, ZML, JY performed the experimental work. YKH conceived and designed the study.
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Zhang, RY., Li, WF., Huang, YK. et al. Molecular Identification of Sugarcane White Leaf in Puer, Yunnan Province, China. Sugar Tech 21, 734–736 (2019). https://doi.org/10.1007/s12355-019-00721-0
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DOI: https://doi.org/10.1007/s12355-019-00721-0