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Molecular Identification of Sugarcane White Leaf in Puer, Yunnan Province, China

  • Rong-Yue Zhang
  • Wen-Feng Li
  • Ying-Kun HuangEmail author
  • Xiao-Yan Wang
  • Hong-Li Shan
  • Jie Li
  • Xiao-Yan Cang
  • Zhi-Ming Luo
  • Jiong Yin
Short Communication
  • 28 Downloads

Abstract

To confirm the phytoplasma association in 13 sugarcane white leaf (SCWL) symptomatic samples collected from Puer, China, nested PCR assays with phytoplasma universal primer pairs was performed. A ~ 1240 bp product was obtained from 10 out of 13 symptomatic samples, and 16S rRNA gene sequencing results showed that all sequences of SCWL isolates were 100% identical to each other. BLASTn analysis revealed 100% sequence similarity with the 16S rRNA genes from SCWL phytoplasma strains LC7 and LC9 from Lincang, Yunnan province, China belonging to 16SrXI-B subgroup. Virtual restriction fragment length polymorphism also confirmed that Puer SCWL strains were member of the 16SrXI-B subgroup.

Keywords

Yunnan province Puer Phytoplasma Sugarcane white leaf 16SrXI-B 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (31760504), Yunnan Agricultural Foundation Projects [2017FG001(-054)], the Sugar Crop Research System (CARS-170303), Yunling Industry and Technology Leading Talent Training Program “Prevention and Control of Sugarcane Pests” (2018LJRC56), and the Yunnan province Agriculture Research System (YNGZTX-4-92).

Authors’ Contributions

RYZ analyzed the sequences and wrote the manuscript. WFL, XYW, JL, HLS, XYC, ZML, JY performed the experimental work. YKH conceived and designed the study.

Compliance with Ethical Standards

Conflicts of interest

The authors declare no conflicts of interest.

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Copyright information

© Society for Sugar Research & Promotion 2019

Authors and Affiliations

  • Rong-Yue Zhang
    • 1
  • Wen-Feng Li
    • 1
  • Ying-Kun Huang
    • 1
    Email author
  • Xiao-Yan Wang
    • 1
  • Hong-Li Shan
    • 1
  • Jie Li
    • 1
  • Xiao-Yan Cang
    • 1
  • Zhi-Ming Luo
    • 1
  • Jiong Yin
    • 1
  1. 1.Sugarcane Research InstituteYunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic ImprovementKaiyuanPeople’s Republic of China

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