Abstract
Sugarcane leaf scald caused by Xanthomonas albilineans (Ashby) Dowson is an extremely destructive disease. In 2017, suspected symptoms of sugarcane leaf scald was observed, in Baoshan, Mengding and Jinping, Yunnan Province, China. The bacteria was isolated from the symptomatic stalks and was subsequently identified as the causal agent using Koch’s postulates. BLASTN analysis showed that the amplified nucleotide sequences of 16S rRNA, gyrB and rpoD genes from all isolates shared 100% sequence identity with the nucleotide sequences of corresponding genes of X. albilineans strain GPE PC73 (Acc no. FP565176). Based on symptom diagnosis in the field, colony morphology, validation of Koch’s postulates and molecular identification of 16S rRNA, gyrB and rpoD genes, the disease was confirmed as sugarcane leaf scald caused by X. albilineans. Partial 16S rRNA, gyrB and rpoD gene sequence analysis of 36 X. albilineans strains from Yunnan and Guangxi provinces, China, was determined. The results revealed that the gyrB gene was the most sensitive in differentiating X. albilineans strains from Yunnan and Guangxi provinces.
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Acknowledgements
This work was supported by the Sugar Crop Research System (CARS-170303), Yunling industry and technology leading talent training program ‘Prevention and Control of Sugarcane Pests’ (2018LJRC56) and Yunnan Province Agriculture Research System (YNGZTX-4-92).
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RYZ wrote the manuscript. XYW analyzed the date. XYW, WFL, JL, HLS, XYC, ZML and JY performed experimental work. YKH conceived and designed the study.
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Zhang, RY., Wang, XY., Shan, HL. et al. Identification and Phylogenetic Analysis of Xanthomonas albilineans (Ashby) Dowson Based on Multiple Gene Sequences in Yunnan Province, China. Sugar Tech 21, 794–801 (2019). https://doi.org/10.1007/s12355-019-00713-0
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DOI: https://doi.org/10.1007/s12355-019-00713-0