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Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for Rapid Diagnosis of Sugarcane yellow leaf virus in Sugarcane

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Abstract

Loop-mediated isothermal amplification (LAMP) is a rapid, highly specific and reliable technique, which is widely incorporated in the field for diagnosis of pathogens at their early stage of infection. In present study, RT-LAMP assay was developed successfully to detect Sugarcane yellow leaf virus (SCYLV) causing yellow leaf disease in sugarcane and compared with conventional reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time polymerase chain reaction (qRT-PCR) to assess its sensitivity. RT-LAMP was able to detect the SCYLV up to a limit of 10 pg (picogram), while RT-PCR assays were able to detect this virus up to 100 pg only. The sensitivity of the RT-LAMP method was tenfold higher than that of conventional RT-PCR assay. This study revealed high sensitivity of RT-LAMP in detecting SCYLV in Indian sugarcane cultivars.

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Acknowledgements

The authors thank the Director of ICAR-SBI, Coimbatore for providing the facilities. The research work was partly funded by ICAR-CRP on Vaccines and Diagnostics project.

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Authors and Affiliations

  1. Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, 641007, India

    L. Anandakumar, K. Bagyalakshmi, K. Nithya & R. Viswanathan

  2. Department of Agricultural and Food Engineering, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India

    L. Anandakumar

  3. ICAR-Sugarcane Breeding Institute Regional Centre, Karnal, 132001, India

    B. Parameswari

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  1. L. Anandakumar
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  2. K. Bagyalakshmi
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  3. K. Nithya
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  4. B. Parameswari
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  5. R. Viswanathan
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Correspondence to R. Viswanathan.

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Anandakumar, L., Bagyalakshmi, K., Nithya, K. et al. Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for Rapid Diagnosis of Sugarcane yellow leaf virus in Sugarcane. Sugar Tech 20, 708–716 (2018). https://doi.org/10.1007/s12355-018-0606-2

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