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Isolation, Transformation and Overexpression of Sugarcane SoP5CS Gene for Drought Tolerance Improvement

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Abstract

Drought is one of the very important growth-limiting factors for sugarcane production in China. Δ1-pyrroline-5-carboxylate synthase (P5CS) is the rate-limiting enzyme in proline synthesis, and it plays an important role in plant response to drought stress. In this study, sugarcane P5CS (SoP5CS) gene was cloned and submitted in GenBank with the accession number KJ546350. The gene SoP5CS was 2151 bp in length, encoding 716 amino acids with a predicted molecular weight of 77.73 kDa and an isoelectric point of 6.14. The qRT-PCR analysis showed that the expression of SoP5CS was higher in leaf than in root and stalk, and strongly induced under ABA, PEG, NaCl and 4 °C treatments. The plant excessive expression vector of SoP5CS was built and transformed into sugarcane calli by Agrobacterium-mediated transformation method, and transgenic sugarcane plants were obtained. Under drought stress, some transgenic lines with overexpressed SoP5CS showed significantly higher in SoP5CS expression level, proline accumulation, abscisic acid content, superoxide dismutase activity and relative water content but lower MDA content and chlorophyll (SPAD value) decrease as compared with the WT plants. Moreover, all the transgenic plants increased drought stress tolerance. Our findings indicated that SoP5CS plays an important role in response to abiotic stresses and overexpression of SoP5CS can improve drought tolerance in transgenic sugarcane plants.

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Funding

The present study was supported by the grants from the National High Technology Research and Development Program (“863” Program) of China (2013AA102604), International Scientific Cooperation Program of China (2013DFA31600), Guangxi Special Funds for Bagui Scholars and Distinguished Experts (2013-03), Fund of Guangxi Key Laboratory of Sugarcane Genetic Improvement (12-K-05-01), Fund for Guangxi Innovation Teams of Modern Agriculture Technology (gjnytxgxcxtd-03-01) and Fund of Guangxi Academy of Agricultural Sciences (2015YT02).

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  1. Agricultural College, State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, 530005, China

    Jian Li, Thi-Thu Phan, Yong-Xiu Xing & Li-Tao Yang

  2. Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China

    Yang-Rui Li

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  1. Jian Li
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Contributions

JL, YRL and LTY design the experiment, JL, TTP, YXX and LTY conducted the experiment, JL, YRL and LTY wrote the manuscript, YRL revised and finalized the manuscript.

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Correspondence to Yang-Rui Li or Li-Tao Yang.

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Li, J., Phan, TT., Li, YR. et al. Isolation, Transformation and Overexpression of Sugarcane SoP5CS Gene for Drought Tolerance Improvement. Sugar Tech 20, 464–473 (2018). https://doi.org/10.1007/s12355-017-0568-9

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