Abstract
Transposable elements constitute a large fraction of plant genomes, and changes due to TE mobilization have provided powerful genetic and molecular tools. Somaclonal variation is a common phenomena caused either by various extrinsic and intrinsic factors related to in vitro culture or transposon activity has been detected under cell culture or tissue culture milieu. In this study, variability among direct somatic embryogenesis (DSEM) and indirect somatic embryogenesis (ISEM) regenerants of sugarcane cv. CoC-671 was studied using Ac transposon insertion polymorphism. A total of 254 amplification products were obtained ranging from 0.5 Kb to 2 Kb. The DNA profile revealed genetic polymorphism among the ISEM regenerants compared to DSEM regenerants. There was n uniform insertion pattern of the Ac like transposons among the DSEM regenerants, whereas it was variable in case of ISEM regenerants. The results on the insertion polymorphism of Ac homologous regions among the ISEM regenerants indicate that the transposition occurred during in vitro culture and that this marker system could be useful in profiling of genetic variation.
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Suprasanna, P., Manjunatha, B.R., Patade, V.Y. et al. Profiling of culture-induced variation in sugarcane plants regenerated via direct and indirect somatic embryogenesis by using transposon-insertion polymorphism. Sugar Tech 12, 26–30 (2010). https://doi.org/10.1007/s12355-010-0006-8
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DOI: https://doi.org/10.1007/s12355-010-0006-8