Antioxidant and antibacterial evaluation of honey bee hive extracts using in vitro models

  • Syed Ali Raza Naqvi
  • Nasir Mahmood
  • Sumera Naz
  • Zaib Hussain
  • Tauqir A. Sherazi
  • Zulfiqar Ali Khan
  • Sohail Anjum Shahzad
  • Muhammad Yar
  • Iftikhar Hussain Bukhari
  • Matloob Ahmad
  • Asim Mansha
Original Article


Biological evaluation of honey bee hive extracts was estimated using a set of chemical and biological in vitro models. Extractions of bee hives were done in methanol and acetone solvents. Well-tolerated yield was observed in case of acetone solvent (12.34 ± 1.28 %) and methanol solvent (5.98 ± 0.94 %). Total phenolic and total flavonoid contents were found in the range of 359 ± 4.06–755 ± 6.03 and 0.952 ± 0.08–1.266 ± 0.04 mg/100 g dry extract, respectively. Antioxidant potential was tested using most reliable in vitro models, such as DPPH free radical scavenging model, lipid peroxidation inhibition in linoleic acid system and ABTS radical scavenging assay. The DPPH scavenging assay showed 28–55 % radical scavenging, while the peroxidation inhibition in linoleic acid system showed low peroxidation inhibition 6–26 %. TEAC value showed 4.12 ± 0.19 and 3.63 ± 0.5 μM TE/g for methanol and acetone extract, respectively. The antibacterial assay of methanol and acetone extracts showed good zone of inhibition in following order S. aureus (19.1 ± 1.15, 14.9 ± 0.53 mm) >B. subtilis (15.4 ± 0.07, 13.1 ± 0.72 mm) >E. coli (13.8 ± 0.95, 9.9 ± 0.35 mm). Overall result demonstrated promising antioxidant and antibacterial potential of honey bee hive extracts.


Honey bee hive Antioxidants TEAC values S. aureus Lipid peroxidation 


Conflict of interest



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Copyright information

© Springer-Verlag Italia 2013

Authors and Affiliations

  • Syed Ali Raza Naqvi
    • 1
  • Nasir Mahmood
    • 2
  • Sumera Naz
    • 1
  • Zaib Hussain
    • 3
  • Tauqir A. Sherazi
    • 4
  • Zulfiqar Ali Khan
    • 1
  • Sohail Anjum Shahzad
    • 4
  • Muhammad Yar
    • 5
  • Iftikhar Hussain Bukhari
    • 1
  • Matloob Ahmad
    • 1
  • Asim Mansha
    • 1
  1. 1.Department of ChemistryGovernment College UniversityFaisalabadPakistan
  2. 2.Department of Allied Health Sciences and Chemical PathologyUniversity of Health SciencesLahorePakistan
  3. 3.Institute of ChemistryUniversity of the PunjabLahorePakistan
  4. 4.Department of ChemistryCOMSATS Institute of Information TechnologyAbbottabadPakistan
  5. 5.Interdisciplinary Research Centre in Biomedical MaterialsCOMSATS Institute of Information TechnologyLahorePakistan

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