Prevention of diet-induced obesity in C57BL/BJ mice with addition of 2 % dietary green tea but not with cocoa or coffee to a high-fat diet

  • S. A. CichelloEmail author
  • D. P. Begg
  • M. Jois
  • R. S. Weisinger
Original Article


Most of the fat oxidation effects of green tea have been observed in a beverage form. This study explored the use of green tea as feed formation in obese mice to protect against diet-induced obesity. Forty-eight adult C57BL/6J mice were randomised to either a control diet with 36.1 % w/w sucrose or a standard diet, except for the replacement of 2 % w/w of the sucrose dietary component (36.1 % sucrose in control) with 2 % w/w cocoa (34.1 % sucrose), 2 % w/w coffee, or 2 % w/w powdered Japanese green tea. At 16th week, body composition, plasma glucose, organ samples and bone mineral density were obtained. In comparison to the control group, only the 2 % green tea group displayed lower body weight (p < 0.01), body fat, liver weight (p < 0.05), hepatic lipid status and higher lean mass. The coffee group displayed lower body fat (p < 0.05) and the cocoa group lower plasma glucose (p < 0.05), but this was not substantiated by other parameters. Results of study indicate the anti-obesity potential of green tea when consumed as 2 % (w/w %) of the diet probably by lipolysis and decreased systemic inflammation resulting from consumption of a high-fat, high-sucrose diet in obesity-prone strain of mice.


Green tea Caffeine Diet-induced obesity EGCG Catechins 



The corresponding author would like to thank Professor Richard Weisinger for his selfless donation of research funds, equipment and most of all time to enable this project to be completed.

Conflict of interest



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Copyright information

© Springer-Verlag Italia 2013

Authors and Affiliations

  • S. A. Cichello
    • 1
    • 3
    Email author
  • D. P. Begg
    • 2
  • M. Jois
    • 1
  • R. S. Weisinger
    • 2
  1. 1.School of Life SciencesLa Trobe UniversityMelbourneAustralia
  2. 2.School of Psychological SciencesLa Trobe UniversityBundooraAustralia
  3. 3.Key State Laboratory for Pu-erh Tea ResearchYunnan Agricultural UniversityYunnanPeople’s Republic of China

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