Advertisement

Prevention of diet-induced obesity in C57BL/BJ mice with addition of 2 % dietary green tea but not with cocoa or coffee to a high-fat diet

  • S. A. CichelloEmail author
  • D. P. Begg
  • M. Jois
  • R. S. Weisinger
Original Article

Abstract

Most of the fat oxidation effects of green tea have been observed in a beverage form. This study explored the use of green tea as feed formation in obese mice to protect against diet-induced obesity. Forty-eight adult C57BL/6J mice were randomised to either a control diet with 36.1 % w/w sucrose or a standard diet, except for the replacement of 2 % w/w of the sucrose dietary component (36.1 % sucrose in control) with 2 % w/w cocoa (34.1 % sucrose), 2 % w/w coffee, or 2 % w/w powdered Japanese green tea. At 16th week, body composition, plasma glucose, organ samples and bone mineral density were obtained. In comparison to the control group, only the 2 % green tea group displayed lower body weight (p < 0.01), body fat, liver weight (p < 0.05), hepatic lipid status and higher lean mass. The coffee group displayed lower body fat (p < 0.05) and the cocoa group lower plasma glucose (p < 0.05), but this was not substantiated by other parameters. Results of study indicate the anti-obesity potential of green tea when consumed as 2 % (w/w %) of the diet probably by lipolysis and decreased systemic inflammation resulting from consumption of a high-fat, high-sucrose diet in obesity-prone strain of mice.

Keywords

Green tea Caffeine Diet-induced obesity EGCG Catechins 

Notes

Acknowledgments

The corresponding author would like to thank Professor Richard Weisinger for his selfless donation of research funds, equipment and most of all time to enable this project to be completed.

Conflict of interest

None.

References

  1. 1.
    Murase T, Nagasawa A, Suzuki J, Hase T, Tokimitsu I (2002) Beneficial effects of tea catechins on diet-induced obesity: stimulation of lipid catabolism in the liver. Int J Obes 26:1459–1464CrossRefGoogle Scholar
  2. 2.
    Amantana A, Santana–Rios G, Butler JA, Xu MR, Whanger PD, Dashwood RH (2002) Antimutagenic activity of selenium-enriched green tea toward the heterocyclic amine 2-amino-3-methylimidazo[4,5-f]quinoline. Biol Trace Elem Res 86:177–191CrossRefGoogle Scholar
  3. 3.
    Embola CW, Sohn OS, Fiala ES, Weisburger JH (2002) Induction of UDP-glucuronosyltransferase 1 (UDP-GT1) gene complex by green tea in male F344 rats. Food Chem Toxicol 40:841–844CrossRefGoogle Scholar
  4. 4.
    Kondo T, Ohta T, Igura K, Hara Y, Kaji D (2002) Tea catechins inhibit angiogenesis in vitro, measured by human endothelial cell growth, migration and tube formation, through inhibition of VEGF receptor binding. Cancer Lett 180:139–144CrossRefGoogle Scholar
  5. 5.
    Pan TH, Jankovic J, Le WD (2003) Potential therapeutic properties of green tea polyphenols in Parkinson’s disease. Drugs Aging 20:711–721CrossRefGoogle Scholar
  6. 6.
    Cabera C, Artacho R, Gimenez R (2006) Beneficial effects of green tea—a review. J Am Coll Nutr 25:279–299Google Scholar
  7. 7.
    GIA (2011) Global Industry Analysts, Inc. Report on Global Hot Beverages (Coffee and Tea) Market. Available from: http://www.strategyr.com/Hot_Beverages_Coffee_and_Tea_Market_Report.asp (Accessed on 10th July 2013)
  8. 8.
    Basu Majumder A, Bera B, Rajan A (2010) Tea statistics: global scenario. Inc J Tea Sci 8:121–124Google Scholar
  9. 9.
    Ananingsih VK, Sharma A, Zhou W (2013) Green tea catechins during food processing and storage: a review on stability and detection. Food Res Int 50:469–479CrossRefGoogle Scholar
  10. 10.
    Chen ZU, Zhu QY, Tsang D, Huang YJ (2001) Degradation of green tea catechins in tea drinks. Agric. Food Chem 49:477–482Google Scholar
  11. 11.
    Chu Y, Huddleston GG, Clancy AN, Harris RBS, Bartness TJ (2010) Epididymal fat is necessary for spermatogenesis, but not testosterone production or copulatory behavior. Endocrinology 151:5669–5679CrossRefGoogle Scholar
  12. 12.
    Dulloo AG, Duret C, Rohrer D, Girardier L, Mensi N, Fathi M et al (1999) Efficacy of a green tea extract rich in catechin polyphenols and caffeine in increasing 24-h energy expenditure and fat oxidation in humans. Am J Clin Nutr 70:1040–1045Google Scholar
  13. 13.
    Dulloo AG, Montani JP (2012) Body composition, inflammation and thermogenesis in pathways to obesity and the metabolic syndrome: an overview. Obes Rev Suppl 2 pp 1–5Google Scholar
  14. 14.
    Thielecke F, Rahn G, Böhnke J, Adams F, Birkenfeld AL, Jordan J et al (2010) Epigallocatechin-3-gallate and postprandial fat oxidation in overweight/obese male volunteers: a pilot study. Eur J Clin Nutr 64:704–713CrossRefGoogle Scholar
  15. 15.
    Klaus S, Pültz S, Thöne-Reineke C, Wolfram S (2005) Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation. Int J Obes 29:615–623CrossRefGoogle Scholar
  16. 16.
    Choo JJ (2003) Green tea reduces body fat accretion caused by high-fat diet in rats through beta-adrenoceptor activation of thermogenesis in brown adipose tissue. J Nutr Biochem 14:671–676CrossRefGoogle Scholar
  17. 17.
    Hodgson AB, Randell RK, Jeukendrup AE (2013) The effect of green tea extract on fat oxidation at rest and during exercise: evidence of efficacy and proposed mechanisms. Adv Nutr 4:129–140CrossRefGoogle Scholar
  18. 18.
    Han LK, Takaku T, Li J, Kimura Y, Okuda H (1999) Anti-obesity action of oolong tea. Int J Obes 23:98–105CrossRefGoogle Scholar
  19. 19.
    Yang M, Wang C, Chen H (2001) Green, oolong and black tea extracts modulate lipid metabolism in hyperlipidemia rats fed high-sucrose diet. J Nutr Biochem 12:14–20CrossRefGoogle Scholar
  20. 20.
    Hasegawa N, Yamda N, Mori M (2003) Powdered green tea has anti-lipogenic effect on Zucker rats fed a high-fat diet. Phytother Res 17:477–480CrossRefGoogle Scholar
  21. 21.
    Greenberg JA, Axen KV, Schnoll R, Boozer CN (2005) Coffee, tea and diabetes: the role of weight loss and caffeine. Int J Obes 29:1121–1129CrossRefGoogle Scholar
  22. 22.
    Zheng G, Sayama K, Okubo T, Juneja LR, Oguni I (2004) Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice. In Vivo 18:55–62Google Scholar
  23. 23.
    Matsui N, Ito R, Nishimura E, Yoshikawa M, Kato M, Kamei M et al (2005) Ingested cocoa can prevent high-fat diet-induced obesity by regulating the expression of genes for fatty acid metabolism. Nutrition 21:594–601CrossRefGoogle Scholar
  24. 24.
    Anderson RA, Polansky MM (2002) Tea enhances insulin activity. J Agric Food Chem 50:7182–7186CrossRefGoogle Scholar
  25. 25.
    Wu LY, Juan CC, Hsu YP, Hwang LS (2004) Effect of tea green supplementation on insulin sensitivity in Sprague-Dawley rats. J Agric Food Chem 52:643–648CrossRefGoogle Scholar
  26. 26.
    Shen CL, Wang P, Guerrieri J, Yeh JK, Wang JS (2008) Protective effect of green tea polyphenols on bone loss in middle-aged female rats. Osteoporos Int 19:979–990CrossRefGoogle Scholar
  27. 27.
    Devine A, Hodgson JM, Dick IM, Prince RL (2007) Tea drinking is associated with benefits on bone density in older women. Am J Clin Nutr 86:1243–1247Google Scholar
  28. 28.
    Muraki S, Yamamoto S, Ishibashi H, Oka H, Yoshimura N, Kawaguchi H et al (2007) Diet and lifestyle associated with increased bone mineral density: cross-sectional study of Japanese elderly women at an osteoporosis outpatient clinic. J Orthop Sci 12:317–320CrossRefGoogle Scholar
  29. 29.
    Grassi F, Gianluca T, Robbie–Ryan M, Gao Y, Terauchi M, Yang X et al (2007) Oxidative stress causes bone loss in estrogen-deficient mice through enhanced bone marrow dendritic cell activation. PNAS 104:15087–15092CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Italia 2013

Authors and Affiliations

  • S. A. Cichello
    • 1
    • 3
    Email author
  • D. P. Begg
    • 2
  • M. Jois
    • 1
  • R. S. Weisinger
    • 2
  1. 1.School of Life SciencesLa Trobe UniversityMelbourneAustralia
  2. 2.School of Psychological SciencesLa Trobe UniversityBundooraAustralia
  3. 3.Key State Laboratory for Pu-erh Tea ResearchYunnan Agricultural UniversityYunnanPeople’s Republic of China

Personalised recommendations