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Effect of combined vitamins C and E supplementation on oxidant/antioxidant status in hemodialysis patients

  • Original Article
  • Published:
Mediterranean Journal of Nutrition and Metabolism

Abstract

The aim of this study was to evaluate the antioxidant supplementation effect on antioxidant system in hemodialysis patients. The study group consisted of 38 patients undergoing hemodialysis and 72 healthy subjects. The patients orally received combination of vitamins C (250 mg/time) and E (200 IU/time) three times per week for 2 months. Both, enzymatic antioxidants [superoxide dismutase (SOD) and glutathione peroxidase (GPx)] activities and non-enzymatic (vitamins E and C) levels and malondialdehyde (MDA) concentrations as lipid peroxidation product in patients were measured before and after supplementation compared with control group. The levels of antioxidants (vitamin E, C and SOD) (P < 0.0001) and GPx (P < 0.05) were significantly enhanced and MDA concentrations declined (P < 0.0001) during the supplementation period. The study demonstrates that combined supplementation of vitamins C and E can noticeably affect antioxidant status and protect against oxidative stress caused during hemodialysis.

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References

  1. Durak I, Kacmaz M, Elgun S, Ozturk HS (2004) Oxidative stress in patients with chronic renal failure: effects of hemodialysis. Med Princ Pract 13:84–87

    Article  Google Scholar 

  2. Ferretti G, Bacchetti T, Masciangelo S, Pallotta G (2008) Lipid peroxidation in hemodialysis patients: effect of vitamin C supplementation. Clin Biochem 41:381–386

    Article  CAS  Google Scholar 

  3. Miyazaki H, Matsuoka H, Itabe H, Usui M, Ueda S, Okuda S, Imaizumi T (2000) Hemodialysis impairs endothelial function via oxidative stress: effects of vitamin E-coated dialyzer. Circulation 101:1002–1006

    CAS  Google Scholar 

  4. Morena M, Cristol JP, Bosc JY, Tetta C, Forret G, Leger CL, Delcourt C, Papoz L, Descomps B, Canaud B (2002) Convective and diffusive losses of vitamin C during haemodiafiltration session: a contributive factor to oxidative stress in haemodialysis patients. Nephrol Dial Transplant 17:422–427

    Article  CAS  Google Scholar 

  5. Giray B, Kan E, Bali M, Hincal F, Basaran N (2003) The effect of vitamin E supplementation on antioxidant enzyme activities and lipid peroxidation levels in hemodialysis patients. Clin Chim Acta 338:91–98

    Article  CAS  Google Scholar 

  6. Luciak M (2004) Antioxidants in the treatment of patients with renal failure. Rocz Akad Med Bialymst 49:157–161

    CAS  Google Scholar 

  7. Bagchi K, Puri S (1998) Free radicals and antioxidants in health and disease. East Mediterr Health J 4:350–360

    Google Scholar 

  8. Papas AM (1999) Antioxidant status, diet, nutrition and health. CRC Press, Boca Raton

    Google Scholar 

  9. Handelman GJ (2000) Evaluation of oxidant stress in dialysis patients. Blood Purif 18:343–349

    Article  CAS  Google Scholar 

  10. Fumeron C, Nguyen-Khoa T, Saltiel C, Kebede M, Buisson C, Drueke TB, Lacour B, Massy ZA (2005) Effects of oral vitamin C supplementation on oxidative stress and inflammation status in haemodialysis patients. Nephrol Dial Transplant 20:1874–1879

    Article  CAS  Google Scholar 

  11. Ha TK, Sattar N, Talwar D, Cooney J, Simpson K, O’Reilly DS, Lean ME (1996) Abnormal antioxidant vitamin and carotenoid status in chronic renal failure. QJM 89:765–769

    Article  CAS  Google Scholar 

  12. Aguilera A, Bajo MA, del Peso G, Diez JJ, Codoceo R, Rebollo F, Mariano M, Selgas R (2002) True deficiency of antioxidant vitamins E and A in dialysis patients. Relationship with clinical patterns of atherosclerosis. Adv Perit Dial 18:206–211

    CAS  Google Scholar 

  13. Mahan LK, Escott-Stump S (2008) Krause’s food nutrition and diet therapy. Sounders Company, Elsevier

  14. Zwolioska D, Grzeszczak W, Szczepaoska M, Kili-Pstrusioka K, Szprynger K (2006) Vitamin A, E and C as non -enzymatic antioxidants band their relation to lipid peroxidation in children with chronic renal failure. Nephron Clin Pract 103:12–18

    Article  CAS  Google Scholar 

  15. Hultqvist M, Hegbran J, Nilsson-Thorell C, Lindholm T, Nilsson P, Linden T, Hultqvist-Bengtsson U (1997) Plasma concentrations of vitamin C, vitamin E and/or malondialdehyde as markers of oxygen free radical production during hemodialysis. Clin Nephrol 47:37–46

    CAS  Google Scholar 

  16. Pastor MC, Sierra C, Bonal J, Teixido J (1993) Serum and erythrocyte tocopherol in uremic patients: effect of hemodialysis versus peritoneal dialysis. Am J Nephrol 13:238–243

    Article  CAS  Google Scholar 

  17. Ziouzenkova O, Asatryan L, Tetta C, Wratten ML, Hwang J, Sevanian A (2002) Oxidative stress during ex vivo hemodialysis of blood is decreased by a novel hemolipodialysis procedure utilizing antioxidants. Free Radic Biol Med 33:248–258

    Article  CAS  Google Scholar 

  18. Huang H-Y, Appel LJ, Croft KD, Miller ER, Mori TA, Puddy IB (2002) Effect of vitamin C and vitamin E on in vivo lipid peroxidation: results of a randomized controlled trial. Am J Clin Nutr 76:549–555

    CAS  Google Scholar 

  19. Karajibani M, Hashemi M, Montazerifar F, Bolouri A, Dikshit M (2009) The status of glutathione peroxidase, superoxide dismutase, vitamins A, C, E and malondialdehyde in patients with cardiovascular disease in Zahedan, Southeast Iran. J Nut Sci Vitaminol 55:309–316

    Article  CAS  Google Scholar 

  20. Naidoo D, Lux O (1998) The effect of vitamin C and E supplementation on lipid and urate oxidation products in plasma. Nutr Res 18:953–961

    Article  CAS  Google Scholar 

  21. Nagyova A, Krajcovicova-Kudlackova M, Horska A, Smolkova B, Blazicek P, Raslova K, Collons A, Dusinska M (2004) Lipid peroxidation in men after dietary supplementation with a mixture of antioxidant nutrients. Bratisl lek listy 105:277–280

    CAS  Google Scholar 

  22. Pham-Huy LA, He H, Pham-Huy C (2008) Free radicals, antioxidants in disease and health. Int J Biomed Sci 4:89–96

    CAS  Google Scholar 

  23. Hultqvist M, Hegbran TJ, Nilsson-Thorell C, Lindholm T, Nilsson P, Linden T, Hultqvist-Bengtsson U (1997) Plasma concentrations of vitamin C, vitamin E and/or malondialdehyde as markers of oxygen free radical production during hemodialysis. Clin Nephrol 47:37–46

    CAS  Google Scholar 

  24. Sinsakul V, Drake JR, Leavitt JNJ, Harrison BR, Fitch CD (1984) Lack of effect of vitamin E therapy on the anemia of patients receiving hemodialysis. Am J Clin Nutr 39:223–226

    CAS  Google Scholar 

  25. Smith KS, Lee CL, Ridlington JW, Leonard SW, Devaraj S, Traber MG (2003) Vitamin E supplementation increases circulating vitamin E metabolites tenfold in end-stage renal disease patients. Lipids 38:813–819

    Article  CAS  Google Scholar 

  26. Washio K, Inagaki M, Tsuji M, Morio Y, Akiyama S, Gotoh H, Gotoh T, Gotoh Y, Oguchi K (2008) Oral vitamin C supplementation in hemodialysis patients and its effect on the plasma level of oxidized ascorbic acid and Cu/Zn superoxide dismutase, an oxidative stress marker. Nephron Clin Pract 109:49–54

    Article  CAS  Google Scholar 

  27. Mydlik M, Derzsiova K, Racz O, Sipulova A, Lovasova E, Petrovicova J (2001) A modified dialyzer with vitamin E and antioxidant defense parameters. Kidney Int Suppl 78:144–147

    Article  Google Scholar 

  28. Chao JCJ, Yuan MD, Chen PY, Chien SW (2002) Vitamin C and E supplements improve the impaired antioxidant status and decrease plasma lipid peroxides in hemodialysis patients small star, filled. J Nutr Biochem 13:653–663

    Article  CAS  Google Scholar 

  29. De Vriese AS, Borrey D, Mahieu E, Claeys I, Stevens L, Vanhaeverbeke A, Roelens M, Langlois MR (2008) Oral vitamin C administration increases lipid peroxidation in hemodialysis patients. Nephron Clin Pract 108:28–34

    Article  CAS  Google Scholar 

  30. Bhogade RB, Suryakar AN, Joshi NG, Patil RY (2008) Effect of vitamin E supplementation on oxidative stress in hemodialysis patients. Indian J Clin Biochem 23:233–237

    Article  CAS  Google Scholar 

  31. Morena M, Martin-Mateo M, Cristol JP, Canaud B (2001) Rational for antioxidant supplementation in hemodialysis patients. Saudi J Kidney Dis Transpl 12:312–324

    CAS  Google Scholar 

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Acknowledgments

The authors are grateful to Dr. Hooshang Sanadgol (Nephrologist) for his helpful comments, staff and nurses of dialysis section in Zahedan Khatam-Al-Anbia Hospital. We also extend our gratitude to the experts of laboratory of National Nutrition and Food Technology Research Institute, Tehran, Iran, for their kind cooperation and the patients and healthy subjects who willingly participated in the study.

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Department of Nutrition, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    F. Montazerifar & M. Karajibani

  2. Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    M. Hashemi

  3. Division of Biochemistry, Department of Chemistry, Pune University, Pune, 411007, India

    M. Dikshit

Authors
  1. F. Montazerifar
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  2. M. Hashemi
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  3. M. Karajibani
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  4. M. Dikshit
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Corresponding author

Correspondence to F. Montazerifar.

Additional information

F. Montazerifar and M. Dikshit share senior authorship.

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Montazerifar, F., Hashemi, M., Karajibani, M. et al. Effect of combined vitamins C and E supplementation on oxidant/antioxidant status in hemodialysis patients. Mediterr J Nutr Metab 3, 159–163 (2010). https://doi.org/10.1007/s12349-010-0015-x

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  • DOI: https://doi.org/10.1007/s12349-010-0015-x

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