Mediterranean Journal of Nutrition and Metabolism

, Volume 3, Issue 2, pp 137–142 | Cite as

Relationship of monocyte chemoattractant protein 1 (MCP-1) with insulin resistance and body mass index, but not with thermogenetic hormones in obesity

  • Giovanni De Pergola
  • Marcello Sciaraffia
  • Rosalba Amoroso
  • Alida Ammirati
  • Domenico Caccavo
  • Federica Meleca
  • Claudia Centoducati
  • Michele Quaranta
Original Article


Monocyte chemoattractant protein 1 (MCP-1) has been implicated in the recruitment of monocytes to atheroma and of monocytes and macrophages to adipose tissue. The aim of the study was to examine whether MCP-1 levels are associated independently with the main thermogenetic hormones (serum TSH and thyroid hormones and 24-h urinary catecholamines) and insulin resistance in a population mainly represented by overweight and obese women. A cohort of 100 consecutive euthyroid women, aged 18–65 years, and with a wide range of BMI, was examined. Central fat accumulation (indirectly measured by waist circumference), fasting MCP-1 plasma levels, and TSH, FT3, FT4, insulin, glucose, and lipid (cholesterol, HDL-cholesterol and triglyceride) serum concentrations, and 24-h urinary catecholamines were measured. Insulin resistance was estimated by homeostasis model assessment (HOMAIR). MCP-1 levels were directly associated with BMI (p < 0.001), waist circumference (p < 0.001), insulin (p < 0.001), HOMAIR (p < 0.001), diastolic blood pressure (DBP) (p < 0.001), systolic blood pressure (SBP) (p < 0.001), triglycerides (TG) (p < 0.05), and 24-h urinary noradrenaline (p < 0.05), and negatively correlated with HDL-cholesterol (p < 0.01). When a multiple regression analysis was performed with MCP-1 as the dependent variable, and only parameters showing a significant univariate association with MCP-1 were considered as the independent variables, MCP-1 maintained an independent positive association with insulin (p < 0.01), and DBP (p < 0.05). When insulin was replaced by HOMAIR in the regression analysis, MCP-1 maintained an independent positive association with HOMAIR (p < 0.05), DBP (p < 0.05), and BMI (p < 0.05). In conclusion, this study suggests that insulin, BMI, and diastolic blood pressure cooperate independently in increasing MCP-1 levels, whereas thyroid hormones and catecholamines have no apparent influence on this chemokine.


MCP-1 Insulin Thyroid hormones Catecholamines 


Conflict of interest



  1. 1.
    Rot A, von Andrian UH (2004) Chemokines in innate and adaptive host defense: basic chemokinese grammar for immune cells. Annu Rev Immunol 22:891–898CrossRefGoogle Scholar
  2. 2.
    Egashira K (2003) Molecular mechanisms mediating inflammation in vascular disease: special reference to monocyte chemoattractant protein-1. Hypertension 41:834–841CrossRefGoogle Scholar
  3. 3.
    Ikeda U, Ikeda M, Seino Y, Takahashi M, Kasahara T, Kano S et al (1993) Expression of intercellular adhesion molecule-1 on rat vascular smooth muscle cells by pro-inflammatory cytokines. Atherosclerosis 104:61–68CrossRefGoogle Scholar
  4. 4.
    Schecter AD, Rollins BJ, Zhang YJ, Charo IF, Fallon JT, Rossikhina M et al (1997) Tissue factor is induced by monocyte chemoattractant protein-1 in human aortic smooth muscle and THP-1 cells. J Biol Chem 272:285568–285573CrossRefGoogle Scholar
  5. 5.
    Viedt C, Vogel J, Athanasiou T, Shen W, Orth SR, Kubler W et al (2002) Monocyte chemoattractant protein-1 induces proliferation and interleukin-6 production in human smooth muscle cells by differential activation of nuclear factor-kappaB and activator protein-1. Arterioscelr Thromb Vasc Biol 22:914–920CrossRefGoogle Scholar
  6. 6.
    Després JP (2006) Intra-abdominal obesity: an untreated risk factor for type 2 diabetes and cardiovascular disease. J Endocrinol Invest 29(3 suppl):77–82Google Scholar
  7. 7.
    Gustafson B, Hammarstedt A, Andersson CX, Smith U (2007) Inflamed adipose tissue: a culprit underlying the metabolic syndrome and atherosclerosis. Arterioscler Thromb Vasc Biol 27:2276–2283CrossRefGoogle Scholar
  8. 8.
    Sartipy P, Loskutoff DJ (2003) Monocyte chemoattractant protein 1 in obesity and insulin resistance. Proc Natl Acad Sci USA 100:7265–7270CrossRefGoogle Scholar
  9. 9.
    Kanda H, Tateya S, Tamori Y, Kotani K, Hiasa K, Kitazawa R (2006) MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity. J Clin Invest 116:1494–1505CrossRefGoogle Scholar
  10. 10.
    Lumeng CN, Deyoung SM, Bodzin JL, Saltiel AR (2007) Increased inflammatory properties of adipose tissue macrophages recruited during diet-induced obesity. Diabetes 56:16–23CrossRefGoogle Scholar
  11. 11.
    Murdolo G, Hammarstedt A, Sandqvist M, Schmelz M, Herder C, Smith U et al (2007) Monocyte chemoattractant protein-1 in subcutaneous abdominal adipose tissue: characterization of interstitial concentration and regulation of gene expression by insulin. J Clin Endocrinol Metab 92:2688–2695CrossRefGoogle Scholar
  12. 12.
    Chacón MR, Fernández-Real JM, Richart C, Megía A, Gómez JM, Miranda M et al (2007) Monocyte chemoattractant protein 1 in obesity and type 2 diabetes. Insulin sensitivity study. Obesity 15:664–672CrossRefGoogle Scholar
  13. 13.
    Bruun JM, Lihn AS, Pedersen SB, Richelsen B (2005) Monocyte chemoattractant protein-1 release is higher in visceral than in subcutaneous adipose tissue (AT): implication of macrophages resident in the AT. J Clin Endocrinol Metab 90:2282–2289CrossRefGoogle Scholar
  14. 14.
    Christiansen T, Richelsen B, Bruun JM (2005) Monocyte chemoattractant protein-1 is produced in isolated adipocytes, associated with adiposity and reduced after weight loss in morbid obese subjects. Int J Obes Relat Metab Disord 29:146–150CrossRefGoogle Scholar
  15. 15.
    Harman-Boehm I, Bluher M, Redel H, Sion-Vardy N, Ovadia S, Avinoach E et al (2007) Macrophage infiltration into omental versus subcutaneous fat across different populations: effect of regional adiposity and the comorbidities of obesity. J Clin Endocrinol Metab 92:2240–2247CrossRefGoogle Scholar
  16. 16.
    Deo R, Khera A, McGuire DK, Murphy SA, Meo Neto Jde P, Morrow DA et al (2004) Association among plasma levels of monocyte chemoattractant protein-1, traditional cardiovascular risk factors, and subclinical atherosclerosis. J Am Coll Cardiol 44:1812–1818CrossRefGoogle Scholar
  17. 17.
    Herder C, Baumert J, Thorand B, Martin S, Löwel H, Kolb H et al (2006) Chemokines and incident coronary heart disease: results from the MONICA/KORA Ausburg Cae-Cohort Study, 1984–2002. Arterioscler Thromb Vascular Biol 26:2147–2152CrossRefGoogle Scholar
  18. 18.
    Herder C, Muller-Scholze S, Rating P, Koenig W, Thorand B, Haastert B et al (2006) Systemic monocyte chemoattractant protein-1 concentrations are independent of type 2 diabetes or parameters of obesity: results from the Cooperative Health Research in the Region of Augsburg Survey (KORA S4). Eur J Endocrinol 154:311–317CrossRefGoogle Scholar
  19. 19.
    Tang W, Pankow JS, Carr JJ, Tracy RP, Bielinski SJ, North KE et al (2007) Association of sICAM-1 and MCP-1 with coronary artery calcification in families enriched for coronary heart disease or hypertension: the NHLBI Family heart Study. BMC Cardiovasc Disord 7:30–41CrossRefGoogle Scholar
  20. 20.
    Kim CS, Park HS, Kawada T, Kim JH, Lim D, Hubbard NE et al (2006) Circulating levels of MCP-1 and IL-8 are elevated in human obese subjects and associated with obesity-related parameters. Int J Obes 30:1347–1355CrossRefGoogle Scholar
  21. 21.
    Asberg M, Nygren A, Leopardi R, Rylander G, Peterson U, Wilczek L et al (2009) Novel biochemical markers of psychosocial stress in women. PLoS One 4:e3590CrossRefGoogle Scholar
  22. 22.
    De Pergola G, Ciampolillo A, Paolotti S, Trerotoli P, Giorgino R (2007) Free triiodothyronine and thyroid stimulating hormone are associated with waist circumference, independently of insulin resistance, metabolic parameters and blood pressare in overweight and obese women. Clin Endocrinol 67:265–269CrossRefGoogle Scholar
  23. 23.
    De Pergola G, Giorgino F, Benigno R, Guida P, Giorgino R (2008) Independent influence of insulin, catecholamines, and thyroid hormones on metabolic syndrome. Obesity 16:2405–2411CrossRefGoogle Scholar
  24. 24.
    Alvarez GE, Beske SD, Ballard TP, Davy KP (2002) Sympathetic neural activation in visceral obesity. Circulation 106:2533–2536CrossRefGoogle Scholar
  25. 25.
    Busetto L, Perini P, Giantin V, Valente P, Segato G, Belluco C et al (1995) Relationship between energy expenditure and visceral fat accumulation in obese women submitted to adjustable silicone gastric banding (ASGB). Int J Obes Relat Metab Disord 19:227–233Google Scholar
  26. 26.
    Leenen R, Van der Kooy K, Deurenberg P, Seidell JC, Weststrate JA, Schouten FJ et al (1992) Visceral fat accumulation in obese subjects: relation to energy expenditure and response to weight loss. Am J Physiol 263:E913–E919Google Scholar
  27. 27.
    Bonora E, Targher G, Alberighe M, Bonadonna RC, Saggiani F, Zenere MB et al (2000) Homeostasis model assessment closely mirrors the glucose clamp technique in the assessment of insulin sensitivity: studies in subjects with various degrees of glucose tolerance and insulin sensitivity. Diabetes Care 23:57–63CrossRefGoogle Scholar
  28. 28.
    Westerbacka J, Cornér A, Kolak M, Makkonen J, Turpeinen U, Hamsten A et al (2008) Insulin regulation of MCP-1 in human adipose tissue of obese and lean women. Am J Physiol Endocrinol Metab 294:E841–E845CrossRefGoogle Scholar
  29. 29.
    Westerbacka J, Corner A, Kannisto K, Kolak M, Makkonen J, Korsheninnikova E et al (2006) Acute in vivo effects of insulin on gene expression in adipose tissue in insulin-resistant and insulin-sensitive subjects. Diabetologia 49:132–140CrossRefGoogle Scholar
  30. 30.
    de Lemos JA, Moerrow DA, Sabatine MS, Murphy SA, Gibson CM, Antman EM et al (2003) Association between plasma levels of monocyte chemoattractant protein-1 and long-term clinical outcomes in patients with acute coronary syndromes. Circulation 107:690–695CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Giovanni De Pergola
    • 1
    • 4
  • Marcello Sciaraffia
    • 2
  • Rosalba Amoroso
    • 1
  • Alida Ammirati
    • 1
  • Domenico Caccavo
    • 1
  • Federica Meleca
    • 1
  • Claudia Centoducati
    • 1
  • Michele Quaranta
    • 3
  1. 1.Internal Medicine, Endocrinology, Andrology and Metabolic Diseases, Department of Emergency and Organ Transplantation, School of Medicine, PoliclinicoUniversity of BariBariItaly
  2. 2.Endocrinology SectionOspedale S.S. AnnunziataTarantoItaly
  3. 3.Laboratorio Analisi, IRCCSOspedale OncologicoBariItaly
  4. 4.BariItaly

Personalised recommendations