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Effect of acrylamide on chick embryonic liver glutathione S-transferases

Mediterranean Journal of Nutrition and Metabolism volume 3, pages 31–38 (2010)Cite this article

Abstract

Glutathione S-transferases (GSTs, EC 2.5.1.18), as the detoxifying enzymes, play an important protective role in embryonic tissues. The GSTs of eleventh-day chick embryonic liver were purified to electrophoretic homogeneity with an overall yield of 31%. The SDS-PAGE analysis of chick embryonic liver GSTs resolved in to three bands, CL1, CL2 and CL3, with relative molecular weights of 27.0, 26.0 and 25.0 kDa respectively. On 72 hours of treatment with acrylamide (AC) (0.1, 0.2 and 0.3 mg) the specific activities of GSTs with CDNB, pNBC, EPNP, BSP, Δ55A and pNPA increased significantly. Total GPx levels were decreased with CHP in liver samples. Immunoblot analysis of AC-treated liver samples showed a dose-dependent increase in the induction of GSTs. CL1 of theta class, CL2 of Mu class and CL3 of alpha class were induced with AC treatment. The theta, mu and alpha classes induced by AC might inhibit the initiation of carcinogenesis.

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Authors and Affiliations

  1. Department of Biochemistry, Sri Venkateswara University, Tirupati, Andhra Pradesh, 517502, India

    Ruxana Begum Sheikh & Thyagaraju Kedam

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  1. Ruxana Begum Sheikh
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  2. Thyagaraju Kedam
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Correspondence to Ruxana Begum Sheikh.

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Begum Sheikh, R., Kedam, T. Effect of acrylamide on chick embryonic liver glutathione S-transferases. Mediterr J Nutr Metab 3, 31–38 (2010). https://doi.org/10.1007/s12349-009-0070-3

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  • DOI: https://doi.org/10.1007/s12349-009-0070-3

Keywords

  • Glutathione S-transferases
  • Acrylamide
  • Substrate specificities
  • Chick embryonic liver
  • Western blot