Abstract
Intercellular influences are necessary for coordinated development and function of vascular and neural components in the brain. In the early postnatal period after birth, the mammalian cerebellum undergoes extensive morphogenesis — developing its characteristic lobules, organizing its diverse cell types into defined cellular layers, and establishing neural circuits that support cerebellar function, such as coordinated movement. In parallel, the cerebellar vasculature undergoes extensive postnatal growth and maturation, keeping pace with the expanding neural compartment. Endothelial deletion of Rbpj leads to neurovascular abnormalities in mice, including arteriovenous (AV) shunts that supplant capillaries and instead direct high-pressure/high-flow arterial blood directly to veins. Gross and histopathological cerebellar abnormalities, associated with these Rbpj-mediated brain AV malformations (AVMs), led to our hypothesis that early postnatal morphogenesis and lamination of cerebellum was perturbed in mice harboring endothelial Rbpj deficiency from birth. Here, we show that endothelial Rbpj-mutant mice developed enlarged vascular malformations on the cerebellar surface, by 2-week post-Rbpj deletion. In addition, outgrowth of cerebellar lobules was impaired through decreased cell proliferation, but not increased apoptosis, in the external granule layer. Molecular layer thickness was reduced, and the Purkinje layer was affected, by decreased Purkinje cell number, primary dendrite length, and dendritic arbor density. Endothelial deletion of Rbpj also led to impaired motor behaviors, consistent with abnormal cerebellar morphogenesis and lamination. Thus, our data suggest that Rbpj is required, in early postnatal vascular endothelium, to ensure proper cerebellar outgrowth, morphogenesis, and function in mice.
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Acknowledgements
We thank Kayleigh Fanelli for assistance with confocal imaging; Ohio University IACUC and Laboratory for Animal Research for animal care; Ohio University Histopathology Core for access to cryostat; and Ohio University Neuroscience Program for access to confocal microscope.
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This research was supported by Ohio University Honors Tutorial College Research Apprenticeship to A.D.C.; Ohio University Summer Neuroscience Undergraduate Research Fellowships to A.L.-B., S.S., and T.R.W.; and start-up funding from Ohio University College of Arts & Sciences to C.M.N.
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A.D.C. and C.M.N. conceptualized and designed experiments; A.D.C., S.S., J.L., A.L.-B., T.R.W., S.A., and C.M.N. performed experiments and analyzed data; and A.D.C. and C.M.N. wrote the manuscript.
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Chapman, A.D., Selhorst, S., LaComb, J. et al. Endothelial Rbpj Is Required for Cerebellar Morphogenesis and Motor Control in the Early Postnatal Mouse Brain. Cerebellum 22, 613–627 (2023). https://doi.org/10.1007/s12311-022-01429-w
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DOI: https://doi.org/10.1007/s12311-022-01429-w