Centrosome Inheritance Does Not Regulate Cell Fate in Granule Neuron Progenitors of the Developing Cerebellum
An inherent asymmetry exists between the two centrosomes of a dividing cell. One centrosome is structurally more mature (mother centrosome) than the other (daughter centrosome). Post division, one daughter cell inherits the mother centrosome while the other daughter cell inherits the daughter centrosome. Remarkably, the kind of centrosome inherited is associated with cell fate in several developmental contexts such as in radial glial progenitors in the developing mouse cortex, Drosophila neuroblast divisions and in Drosophila male germline stem cells. However, the role of centrosome inheritance in granule neuron progenitors in the developing cerebellum has not been investigated. Here, we show that mother and daughter centrosomes do exist in these progenitors, and the amount of pericentriolar material (PCM) each centrosome possesses is different. However, we failed to observe any correlation between the fate adopted by the daughter cell and the nature of centrosome it inherited.
KeywordsMother centrosome Pericentriolar material (PCM) Cell fate Cerebellar granule neuron progenitors (CGNPs)
The authors thank Parthiv Haldipur and Ajit Ray (National Brain Research Centre, India) for their inputs.
SM received funding from the Indo-French Centre for the Promotion of Advanced Research (CEFIPRA, grant no. 4903-02). KC received financial support from the Department of Biotechnology, Government of India (DBT Neurobiology Task Force, grant no. 0376). NR was supported by SwarnaJayanti Fellowship from the Department of Science and Technology, Government of India, and the DBT-IISc partnership program. AC received financial support from the Council of Scientific and Industrial Research (CSIR), Government of India.
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Conflict of Interest
The authors declare that they have no conflict of interest.