Self-Organized Cerebellar Tissue from Human Pluripotent Stem Cells and Disease Modeling with Patient-Derived iPSCs

Abstract

Recent advances in the techniques that differentiate induced pluripotent stem cells (iPSCs) into specific types of cells enabled us to establish in vitro cell-based models as a platform for drug discovery. iPSC-derived disease models are advantageous to generation of a large number of cells required for high-throughput screening. Furthermore, disease-relevant cells differentiated from patient-derived iPSCs are expected to recapitulate the disorder-specific pathogenesis and physiology in vitro. Such disease-relevant cells will be useful for developing effective therapies. We demonstrated that cerebellar tissues are generated from human PSCs (hPSCs) in 3D culture systems that recapitulate the in vivo microenvironments associated with the isthmic organizer. Recently, we have succeeded in generation of spinocerebellar ataxia (SCA) patient-derived Purkinje cells by combining the iPSC technology and the self-organizing stem cell 3D culture technology. We demonstrated that SCA6-derived Purkinje cells exhibit vulnerability to triiodothyronine depletion, which is suppressed by treatment with thyrotropin-releasing hormone and Riluzole. We further discuss applications of patient-specific iPSCs to intractable cerebellar disease.

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Acknowledgements

This work was supported by grant-in-aid from Ministry of Health, Labour and Welfare, grant-in-aid for Scientific Research (C) from Japan Society for the Promotion of Science (JSPS), and the Core Program for Disease Modeling using iPS Cells from JST and AMED.

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Correspondence to Keiko Muguruma.

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Muguruma, K. Self-Organized Cerebellar Tissue from Human Pluripotent Stem Cells and Disease Modeling with Patient-Derived iPSCs. Cerebellum 17, 37–41 (2018). https://doi.org/10.1007/s12311-017-0905-2

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Keywords

  • Purkinje cells
  • Pluripotent stem cells
  • Spinocerebellar ataxia
  • Disease modeling
  • Brain organoid
  • Self-organization
  • Cerebellar development