Migration of Interneuron Precursors in the Nascent Cerebellar Cortex
- 196 Downloads
The cerebellum arguably constitutes one of the best characterized central nervous circuits, and its structure, cellular function, and histogenesis have been described in exceptional quantitative detail. A notable exception to this is the development of its inhibitory interneurons, and in particular the extensive migrations of future basket and stellate cells. Here, we used acute slices from 8-day-old mice to assess the migration of Pax2-EGFP-tagged precursors of these cells en route to the molecular layer during their transit through the nascent cerebellar cortex. We document that movement of these cells is highly directed. Their speed and directional persistence are larger in the nascent granule cell layer than in the molecular layer. And they migrate periodically, with periods of effective, directed translocation separated by bouts of rather local movement. Finally, we document that the arrangement of these cells in the adult molecular layer is characterized by clustering. These data are discussed with a focus on potential generative mechanisms for the developmental pattern observed.
KeywordsCerebellum Inhibitory interneuron Cell migration Pax2 Development Mouse
This work was supported by grants of the German Research Foundation [SPP1757: STE 552/5 to C.S., JA 942-1/2 to RJ] and the European Union [ERA-NET NEURON: BrIE to C.S.].
Compliance with Ethical Standards
The authors declare that they have no competing interests.
- 1.Baddeley A, Rubak E, Turner R. Spatial point patterns: methodology and applications with R. London: Chapman and Hall/CRC; 2015.Google Scholar
- 3.Batschelet E. Circular statistics in biology. London. New York: Academic Press; 1981.Google Scholar
- 15.Neyman J, Scott EL. Statistical approach to problems of cosmology. J R Stat Soc Ser B Methodol. 1958;20:1–43.Google Scholar
- 17.R Core Team. R: a language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing (2017) ISBN 3–900051–07-0; URL http://www.R-project.org/.
- 20.Ramon y Cajal, S (1909) Histologie du système nerveux de l'homme et des vertébrés. II. Paris: A. Maloine.Google Scholar
- 28.Wefers AK, Haberlandt C, Tekin NB, Fedorov DA, Timmermann A, van der Want JJL, et al. Synaptic input as a directional cue for migrating interneuron precursors. Development. 2017;144:4125–36.Google Scholar
- 31.Yau CY, Loh JM. A generalization of the Neyman-Scott process. Stat Sin. 2012;22:1717–36.Google Scholar