Abstract
Phytanic acid (Phyt) brain concentrations are highly increased in Refsum disease, a peroxisomal disorder clinically characterized by neurological features, cardiac abnormalities, and retinitis pigmentosa. Considering that the pathogenesis of cerebellar ataxia, a common finding in this disease, is still unknown, in the present work we investigated the in vitro effects of Phyt at concentrations similar to those found in affected patients on important parameters of mitochondrial homeostasis in cerebellum from young rats. The respiratory parameters states 3 and 4 and respiratory control ratio (RCR) determined by oxygen consumption, membrane potential (∆Ψm), NAD(P)H pool content, and swelling were evaluated in mitochondrial preparations from this cerebral structure. Phyt markedly increased state 4 respiration, whereas state 3 respiration, the RCR, the mitochondrial matrix NAD(P)H content, and ∆Ψm were decreased by this fatty acid, being the latter effect partially prevented by N-acetylcysteine. These data indicate that Phyt behaves as an uncoupler of oxidative phosphorylation and as a metabolic inhibitor disrupting mitochondrial homeostasis in cerebellum. It is proposed that these pathomechanisms may contribute at least in part to the cerebellar alterations found in Refsum disease.
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Abbreviations
- ANT:
-
Adenine nucleotide translocator
- ATC:
-
Atractyloside
- NAC:
-
N-acetylcysteine
- Phyt:
-
Phytanic acid
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Acknowledgments
We are grateful to the financial support of CNPq, CAPES, PROPESq/UFRGS, FAPERGS, PRONEX, FINEP Rede Instituto Brasileiro de Neurociência (IBN-Net) # 01.06.0842-00, and Instituto Nacional de Ciência e Tecnologia- Excitotoxicidade e Neuroproteção (INCT-EN).
Financial Support
CNPq, PROPESq/UFRGS, FAPERGS, PRONEX, FINEP Rede Instituto Brasileiro de Neurociência (IBN-Net) # 01.06.0842-00, and Instituto Nacional de Ciência e Tecnologia-Excitotoxicidade e Neuroproteção (INCT-EN).
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The authors declare that there are no potencial conflicts of interest.
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Busanello, E.N.B., Amaral, A.U., Tonin, A.M. et al. Disruption of Mitochondrial Homeostasis by Phytanic Acid in Cerebellum of Young Rats. Cerebellum 12, 362–369 (2013). https://doi.org/10.1007/s12311-012-0426-y
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DOI: https://doi.org/10.1007/s12311-012-0426-y