The precerebellar nuclei are hindbrain and spinal cord centers that send fibers to the cerebellum. The neurons of the major hindbrain precerebellar nuclei are derived from the rhombic lip. Wnt1, a developmentally important gene involved in intercellular signaling, is expressed in the developing rhombic lip. We sought to investigate the relationship between the cell clusters expressing Wnt1 and the precerebellar nuclei in the hindbrain. We therefore defined the hindbrain precerebellar nuclei by retrograde tracing, following cerebellar injections of HRP, and compared these results with the cell clusters expressing Wnt1 in newborn mice. We found that 39 distinct hindbrain nuclei project to the cerebellum. Of these nuclei, all but three (namely the oral pontine reticular nucleus, the caudal pontine reticular nucleus, and the subcoeruleus nucleus) contain neurons expressing Wnt1. This shows a high degree of overlap between the precerebellar nuclei and the nuclei that express Wnt1. However, it should be noted that neurons expressing Wnt1 are also found in the superior olivary complex, which is a basal plate derivative lacking cerebellar projections.
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Lobules 4 and 5 of the cerebellar vermis
Motor trigeminal nucleus
Motor trigeminal nucleus, parvicellular part
Trigeminal-solitary transition zone
Trigeminal transition zone
Lobule 6 of the cerebellar vermis
Lobule 7 of the cerebellar vermis
Lobule 8 of the cerebellar vermis
Crus1 of the ansiform lobule
Crus2 of the ansiform lobule
Dorsal cochlear nucleus
Dorsomedial spinal trigeminal nucleus
Dorsal raphe nucleus
External cuneate nucleus
Gigantocellular reticular nucleus
Inferior olivary nucleus
Inferior olive dorsal nucleus
Inferior olive dorsomedial cell group
Inferior olive medial nucleus
Inferior olive principal nucleus
Intermediate reticular zone
Longitudinal fasiculus of the pons
Lateral parabrachial nucleus
Lateral parabrachial nucleus external part
Lateral reticular nucleus
Lateroventral periolivary nucleus
Middle cerebellar peduncle
Medullary reticular nucleus, dorsal part
Medullary reticular nucleus, ventral part
Median raphe nucleus
Medial parabrachial nucleus
Medial vestibular nucleus
Medial vestibular nucleus, magnocellular part
Medial vestibular nucleus, parvicellular part
Medioventral periolivary nucleus
Matrix region of the medulla
Parvicelluar reticular nucleus, alpha part
Parvicelluar reticular nucleus
Paramedian reticular nucleus
Paramedian raphe nucleus
Pontine reticular nucleus, caudal part
Pontine nuclei, dorsolateral part
Pontine nuclei, lateral part
Pontine nuclei, medial part
Pontine reticular nucleus, oral part
Pontine nuclei, peduncular part
Pontine raphe nucleus
Pontine nuclei, ventral part
Principal sensory trigeminal nucleus
Principal sensory trigeminal nucleus dorsomedial part
Principal sensory trigeminal nucleus ventrolateral part
Raphe interpositus nucleus
Raphe magnus nucleus
Nucleus of Roller
Raphe obscurus nucleus
Raphe pallidus nucleus
Reticulotegmental nucleus of the pons
Superior cerebellar peduncle
Spinal trigeminal nucleus, caudal part
Spinal trigeminal nucleus, interpolar part
Spinal trigeminal nucleus, oral part
Spinal vestibular nucleus
Superior vestibular nucleus
Ventral cochlear nucleus posterior part
Decussation of the superior cerebellar peduncle
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This project was supported by the ARC Thinking Systems Initiative TS0669860 and an NHMRC Australia Fellowship to GP. We are grateful for the support from NIH grants (NIH5R01GM021168-34) and Howard Hughes Medical Institute to Dr Mario Capecchi.
Conflicts of Interest Statement
The authors of this manuscript have no potential conflicts of interest in the submission. The manuscript has not been published before and is not under consideration anywhere else. The publication has been approved by all co-authors.
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Fu, Y., Tvrdik, P., Makki, N. et al. Precerebellar Cell Groups in the Hindbrain of the Mouse Defined by Retrograde Tracing and Correlated with Cumulative Wnt1-Cre Genetic Labeling. Cerebellum 10, 570 (2011). https://doi.org/10.1007/s12311-011-0266-1
- Precerebellar nuclei
- Inferior olivary nucleus
- Rhombic lip