Precerebellar Cell Groups in the Hindbrain of the Mouse Defined by Retrograde Tracing and Correlated with Cumulative Wnt1-Cre Genetic Labeling

Abstract

The precerebellar nuclei are hindbrain and spinal cord centers that send fibers to the cerebellum. The neurons of the major hindbrain precerebellar nuclei are derived from the rhombic lip. Wnt1, a developmentally important gene involved in intercellular signaling, is expressed in the developing rhombic lip. We sought to investigate the relationship between the cell clusters expressing Wnt1 and the precerebellar nuclei in the hindbrain. We therefore defined the hindbrain precerebellar nuclei by retrograde tracing, following cerebellar injections of HRP, and compared these results with the cell clusters expressing Wnt1 in newborn mice. We found that 39 distinct hindbrain nuclei project to the cerebellum. Of these nuclei, all but three (namely the oral pontine reticular nucleus, the caudal pontine reticular nucleus, and the subcoeruleus nucleus) contain neurons expressing Wnt1. This shows a high degree of overlap between the precerebellar nuclei and the nuclei that express Wnt1. However, it should be noted that neurons expressing Wnt1 are also found in the superior olivary complex, which is a basal plate derivative lacking cerebellar projections.

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Abbreviations

4/5Cb:

Lobules 4 and 5 of the cerebellar vermis

5N:

Motor trigeminal nucleus

5PC:

Motor trigeminal nucleus, parvicellular part

5Sol:

Trigeminal-solitary transition zone

5Tr:

Trigeminal transition zone

6Cb:

Lobule 6 of the cerebellar vermis

7Cb:

Lobule 7 of the cerebellar vermis

7n:

Facial nerve

7N:

Facial nucleus

8Cb:

Lobule 8 of the cerebellar vermis

Ar:

Arcuate nucleus

C:

Cochlear nuclei

Cb:

Cerebelum

chp:

Choroid plexus

Crus1:

Crus1 of the ansiform lobule

Crus2:

Crus2 of the ansiform lobule

Cu:

Cuneate nucleus

DC:

Dorsal cochlear nucleus

DMSp5:

Dorsomedial spinal trigeminal nucleus

DR:

Dorsal raphe nucleus

ECu:

External cuneate nucleus

Gi:

Gigantocellular reticular nucleus

IC:

Inferior colliculus

In:

Intercalated nucleus

IO:

Inferior olivary nucleus

IOD:

Inferior olive dorsal nucleus

IODM:

Inferior olive dorsomedial cell group

IOM:

Inferior olive medial nucleus

IOPr:

Inferior olive principal nucleus

IRt:

Intermediate reticular zone

KF:

Kölliker-Fuse nucleus

LC:

Locus coeruleus

lfp:

Longitudinal fasiculus of the pons

Li:

Linear nucleus

LPB:

Lateral parabrachial nucleus

LPBE:

Lateral parabrachial nucleus external part

LRt:

Lateral reticular nucleus

LVPO:

Lateroventral periolivary nucleus

mcp:

Middle cerebellar peduncle

MdD:

Medullary reticular nucleus, dorsal part

MdV:

Medullary reticular nucleus, ventral part

ml:

Medial lemniscus

MnR:

Median raphe nucleus

MPB:

Medial parabrachial nucleus

MVe:

Medial vestibular nucleus

MVeMC:

Medial vestibular nucleus, magnocellular part

MVePC:

Medial vestibular nucleus, parvicellular part

MVPO:

Medioventral periolivary nucleus

Mx:

Matrix region of the medulla

PCRtA:

Parvicelluar reticular nucleus, alpha part

PCRt:

Parvicelluar reticular nucleus

PM:

Paramedian lobule

PMn:

Paramedian reticular nucleus

PMnR:

Paramedian raphe nucleus

Pn:

Pontine nuclei

PnC:

Pontine reticular nucleus, caudal part

PnDL:

Pontine nuclei, dorsolateral part

PnL:

Pontine nuclei, lateral part

PnM:

Pontine nuclei, medial part

PnO:

Pontine reticular nucleus, oral part

PnPed:

Pontine nuclei, peduncular part

PnR:

Pontine raphe nucleus

PnV:

Pontine nuclei, ventral part

Pr:

Prepositus nucleus

Pr5:

Principal sensory trigeminal nucleus

Pr5DM:

Principal sensory trigeminal nucleus dorsomedial part

Pr5VL:

Principal sensory trigeminal nucleus ventrolateral part

py:

Pyramidal tract

r:

Rhombomere

RIP:

Raphe interpositus nucleus

RL:

Rhombic lip

RMg:

Raphe magnus nucleus

Ro:

Nucleus of Roller

ROb:

Raphe obscurus nucleus

RPa:

Raphe pallidus nucleus

RtTg:

Reticulotegmental nucleus of the pons

scp:

Superior cerebellar peduncle

Sim:

Simple lobule

Sol:

Solitary nucleus

Sp5C:

Spinal trigeminal nucleus, caudal part

Sp5I:

Spinal trigeminal nucleus, interpolar part

Sp5O:

Spinal trigeminal nucleus, oral part

SpVe:

Spinal vestibular nucleus

SubC:

Subcoeruleus nuclei

SuVe:

Superior vestibular nucleus

VCP:

Ventral cochlear nucleus posterior part

Ve:

Vestibular nuclei

X:

Nucleus X

xscp:

Decussation of the superior cerebellar peduncle

Y:

Nucleus Y

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Acknowledgments

This project was supported by the ARC Thinking Systems Initiative TS0669860 and an NHMRC Australia Fellowship to GP. We are grateful for the support from NIH grants (NIH5R01GM021168-34) and Howard Hughes Medical Institute to Dr Mario Capecchi.

Conflicts of Interest Statement

The authors of this manuscript have no potential conflicts of interest in the submission. The manuscript has not been published before and is not under consideration anywhere else. The publication has been approved by all co-authors.

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Correspondence to Charles Watson.

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Fu, Y., Tvrdik, P., Makki, N. et al. Precerebellar Cell Groups in the Hindbrain of the Mouse Defined by Retrograde Tracing and Correlated with Cumulative Wnt1-Cre Genetic Labeling. Cerebellum 10, 570 (2011). https://doi.org/10.1007/s12311-011-0266-1

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Keywords

  • Hindbrain
  • Precerebellar nuclei
  • Wnt1
  • Inferior olivary nucleus
  • Rhombic lip