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Cancer Microenvironment

, Volume 7, Issue 1–2, pp 71–78 | Cite as

Extracellular Matrix Protein Laminin Induces Matrix Metalloproteinase-9 in Human Breast Cancer Cell Line MCF-7

  • Sekhar Pal
  • Shuvojit Moulik
  • Anindita Dutta
  • Amitava ChatterjeeEmail author
Original Paper

Abstract

Studies on interaction of tumor cells with extracellular matrix (ECM) components showed increased extracellular protease activity mediated by the family of matrix metalloproteinases (MMPs). Here we studied the effect of human breast cancer cell line MCF-7-laminin (LM) interaction on MMPs and the underlying signaling pathways. Culturing of MCF-7 cells on LM coated surface upregulated MMP-9 expression as well as reduced tissue inhibitor of metalloproteinases-1 (TIMP-1) expression. LM induced MMP-9 expression is abrogated by the blockade of α2 integrin. Inhibitor studies indicate possible involvement of phosphatidyl-inositol-3-kinase (PI3K), extracellular signal regulated kinase (ERK) and nuclear factor-kappaB (NF-κB) in LM induced signaling. LM treatment also enhanced phosphorylation of FAK (focal adhesion kinase), PI3K, ERK; nuclear translocation of ERK, pERK, NF-κB and cell migration. Our findings indicate that, binding of MCF-7 cells to LM, possibly via α2β1 integrin, induces signaling involving FAK, PI3K, ERK, NF-κB followed by upregulation of MMP-9 and cell migration.

Keywords

MCF-7 Signaling ECM Laminin Integrin MMP-9 

Notes

Acknowledgments

The authors wish to express their thanks to Director, Chittaranjan National Cancer Institute, for academic, financial and infrastructural support.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Sekhar Pal
    • 1
  • Shuvojit Moulik
    • 1
  • Anindita Dutta
    • 1
  • Amitava Chatterjee
    • 1
    • 2
    Email author
  1. 1.Department of Receptor Biology & Tumor MetastasisChittaranjan National Cancer InstituteKolkataIndia
  2. 2.KolkataIndia

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