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Pyramiding of qDTY1.1 and qDTY3.1 into rice mega-variety Samba Mahsuri-Sub1: physiological performance under water deficit conditions

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Abstract

Water deficit is a crucial factor causing huge loss to rice productivity. The present study aimed to develop a multiple stress tolerant genotype by pyramiding drought tolerant yield QTLs qDTY1.1 and qDTY3.1 into high yielding rice variety Samba Mahsuri Sub-1(SMS) through marker assisted pyramiding. To achieve this six introgression lines of SMS carrying qDTY1.1 (SAB) were crossed with DRR-50, an Essentially Derived Variety of SMS carrying qDTY3.1. The SAB lines are taller than SMS due to tight linkage between qDTY1.1 and wild type SD-1. Therefore, F2 generation of crosses were screened for recombinants between SD-1 and qDTY1.1. Phenotyping of 1530 F2 plants representing three F2 populations from 35 F1 hybrids, identified 305 dwarf plants. Three dwarf F2 plants along with three others carrying qDTY1.1 and qDTY3.1 were forwarded to F3 generation. From the six F3 (SABD) lines fourteen pyramided progenies were selected and forward to F4 generation. The six SABD F3 lines SABD-7, SABD-8, SABD-9, SABD-76, SABD-79 and SABD-80 along with parents were evaluated under moisture stress (MS) for various physiological parameters. Chlorophyll and relative water content were more, while canopy temperature and malonaldehyde (MDA) content were lesser in SABD lines compared to parents indicating tolerance under MS. Variance due to genotypes was highly significant for all the yield related traits except test weight. Based on seed morphology, agronomic characters and physiological parameters six superior lines SABD-9-3, SABD-9-2, SABD-9-6, SABD-9-7, SABD-76-2 and SABD-76-6 performing better under MS were identified, which could be released after multi-location evaluation.

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Acknowledgements

The research work was supported by funding from Department of Biotechnology, Govt. of India (Grant Nos. BT/PR/1454/AGR/02/745/2010; BT/PR18611/AGIII/103/921/2016). DC, DD and BL were supported by scholarships from CAU (Imphal), Indian Council of Agricultural Research (National Talent Scholarship) and University Grants Commission, respectively.

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Author notes

  1. Wricha Tyagi

    Present address: International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Telangana, India

Authors and Affiliations

  1. School of Crop Improvement, College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University (Imphal), Umiam, India

    Mayank Rai, Diezehlouno Chucha, Dake Deepika, Bharati Lap, P. Magudeeswari & Wricha Tyagi

  2. ICAR- Indian Institute of Rice Research, Hyderabad, Telangana, India

    G. Padmavathi

  3. ICAR- National Institute for Plant Biotechnology, New Delhi, India

    Nagendra Singh

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  1. Mayank Rai
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Contributions

MR, NS conceived and supervised the project. WT supervised the molecular work. PG contributed DRR-50 seeds. DC made crosses and performed physiological analysis. DC, DD, BL and MP performed molecular marker analysis. MR and DD selected the dwarf pyramided lines. DC, BL, MP and MR interpreted the data. DC wrote the original draft and WT and MR edited the manuscript. All authors read and approved the final manuscript.

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Correspondence to Wricha Tyagi.

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Supplementary Information

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12298_2023_1387_MOESM1_ESM.pdf

Fig. S1 Confirmation of pyramided lines. a and b, with molecular markers; and c, seed morphology of donor, recipient, single and double pyramided rice lines. d, Variation for morpho-physiological traits of parents and pyramided lines. The F2 homozygotes for QTLs, DTY1.1 and DTY2.2 confirmed using SSR markers, RM327 and RM431, respectively. Desired plants are indicated by * (PDF 1643 kb)

12298_2023_1387_MOESM2_ESM.tif

Fig. S2 Screening of rice plants in control and under low moisture conditions. a, vegetative stage. b, reproductive stage and c, soil moisture profile following withdrawal of water (Mean ± SD, n = 4). MSV- Moisture stress Vegetative, MSR- Moisture stress reproductive (TIF 2594 kb)

12298_2023_1387_MOESM3_ESM.tiff

Fig. S3 Representative chromosomal map and gel pictures for confirmation of pyramided lines. a, Map for chromosomes 1 and 3 showing DTY1.1 and DTY3.1 along with SSR available for foreground and recombinant selection. b and c, Gel pictures showing F2 confirmation with molecular markers. The homozygotes for QTLs, DTY1.1 and DTY3.3 confirmed using SSR markers, RM431 and RM520, respectively. Desired plants are indicated by * (TIFF 3378 kb)

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Rai, M., Chucha, D., Deepika, D. et al. Pyramiding of qDTY1.1 and qDTY3.1 into rice mega-variety Samba Mahsuri-Sub1: physiological performance under water deficit conditions. Physiol Mol Biol Plants 29, 1931–1943 (2023). https://doi.org/10.1007/s12298-023-01387-5

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