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Herbal and chemical seed potentiations improve the redox health of aged seeds of indigenous aromatic rice cultivars through regulation of oxidative window, gene expression, and restoration of hormonal homeostasis

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Abstract

Previous studies associated with seed potentiation support the critical role of metabolic readjustment in restricting the loss of seed vigor and viability of aged seeds. However, their exact role in the regulation of ‘oxidative windows’ of potentiated seeds is rarely studied and hence is the subject of the present investigation. Seed potentiation of two contrasting indigenous aromatic rice cultivars, differing in sensitivity towards redox attributes (Oryza sativa L., Cultivars Tulaipanji and Jamainadu), with standardized doses of hydrogen peroxide (20 mM), triadimefon (250 μM), herbal extract (1% aqueous extract of Lantana camara flower) and distilled water before accelerated aging (RH 92% and 41 °C for 24 h) found to have significant impact on redox regulation of aged seeds and improvement of germination phenotypes. The efficacy of integrated RBOH-ascorbate–glutathione/catalase pathway, redox status and other redox fingerprints in the metabolic landscape of potentiated-aged seeds vis-a-vis non-potentiated-aged seeds corroborate the impact of seed potentiation on the regulation of ‘oxidative window’ of experimental rice seeds. Gene expression analysis of central redox hub enzymes (Osrboh, OsAPx2, OsGRase, OsCatA) strongly substantiates the impact of seed potentiation on transcriptional regulation of genes for redox homeostasis in accelerated aged seeds. The novelty of the current effort is that it suggests a positive nexus between seed potentiation-induced redox regulation and hormonal homeostasis. The efficacy of seed potentiation on the redox regulation of experimental accelerated aged seeds is found to be cultivar-specific and comparatively better in the cultivar Tulaipanji as compared to the cultivar Jamainadu and in the order herbal extract, hydrogen peroxide, hydropriming and triadimefon.

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Acknowledgements

BP acknowledges Council of Scientific and Industrial Research, New Delhi, India (File no. 09/025(0260)/2018-EMR-I) for her research fellowship. The financial help of Department of Science and Technology - Fund for Improvement of S&T Infrastructure (DST – FIST) (SR/FST/LS-1/2018/188©, dated 01.10.2019) for instrumentation facilities, also acknowledged gratefully. SB acknowledges Department of Science and Technology - Science and Engineering Research Board (DST – SERB) (CRG/2021/000513) for financial assistance.

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  1. Plant Physiology and Biochemistry Research Laboratory, UGC Centre for Advanced Study, Department of Botany, University of Burdwan, Burdwan, West Bengal, 713104, India

    Babita Pal & Soumen Bhattacharjee

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Study conception and design was done by SB. Material preparation, data collection and analysis were performed by BP, SB. The first draft of the manuscript was written by SB and all authors commented on previous versions of the manuscript. All authors approved the final manuscript.

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Correspondence to Soumen Bhattacharjee.

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Supplementary Fig. 1

RP-HPLC chromatogram showing changes in endogenous titer of plant hormones (Gibberellic acid, Abscisic acid and Jasmonic acid) of potentiated-accelerated aged seeds [Herbal (c, h), H2O2 (d, i) and Triadimefon (e, j)] vis-a-vis untreated control (a, f) and non-potentiated-accelerated aged seeds (b, g) during early germination of two experimental IARCs (Oryza sativa L., Cultivar Tulaipanji and Jamainadu). Supplementary Fig. 2 Seed potentiation impact [Hydro-primed (c, i), Herbal (d, j), H2O2 (e, k) and Triadimefon (f, l)] of accelerated aged seeds vis-a-vis untreated control (a, g) and non-potentiated-accelerated aged seeds (b, h) on germination phenotypes of two experimental IARCs (Oryza sativa L., Cultivars Tulaipanji and Jamainadu).

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Pal, B., Bhattacharjee, S. Herbal and chemical seed potentiations improve the redox health of aged seeds of indigenous aromatic rice cultivars through regulation of oxidative window, gene expression, and restoration of hormonal homeostasis. Physiol Mol Biol Plants 29, 1269–1288 (2023). https://doi.org/10.1007/s12298-023-01375-9

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