Abstract
There is an increasing interest for plant hormones to modulate the harmful effects of drought on crops. The present study was conducted to assess the effect of foliar-applied cytokin (CK) and abscisic acid (ABA) on yield, organic acids, minerals, and fatty acid profile of wheat (Triticum aestivum L.) cultivars (MV17 and Pishgam) in response to drought stress. The results showed drought significantly decreased grain yield and biomass, but they were enhanced by CK and ABA application. Acetic acid increased under drought stress conditions, and the remarkable increase (~ twofold) in succinic acid content was observed with ABA application under drought stress in MV17 cultivar. In general, drought stress decreased malic acid, pyruvic acid, and citric acid, but CK enhanced them. The leaf accumulations of potassium (K+), calcium (Ca2+), magnesium (Mg2+), iron (Fe2+), and zinc (Zn2+) decreased by drought, where its reduction in MV17 was greater than Pishgam. However, an increased sodium (Na+) content was observed in plants experiencing drought with non-foliar application of ABA and CK. The plant growth hormones especially CK increased K+, Ca2+, Mg2+, Fe2+, and Zn2+, but decreased Na+. Fatty acid profile showed increased polyunsaturated fatty acids and monounsaturated fatty acids upon the drought stress. According to heat map, organic acids represented the maximum variations but fatty acids showed the minimum change during the treatments. The present study recommended foliar-applied CK to alleviate drought stress on wheat yield.
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Aqaei P, Weisany W, Diyanat M, Razmi J, Struik PC (2020) Response of maize (Zea mays L.) to potassium nano-silica application under drought stress. J Plant Nutr 43(9):1205–1216. https://doi.org/10.1080/01904167.2020.1727508
Arndt C, Farmer W, Strzepek K, Thurlow J (2012) Climate change, agriculture and food security in Tanzania. Rev Develop Econ 16(3):378–393
Artner C, Benkova E (2019) Ethylene and cytokinin: partners in root growth regulation. Molecul Plant 12(10):1312–1314
Ashrafi M, Azimi-Moqadam MR, Moradi P, MohseniFard E, Shekari F, Kompany-Zareh M (2018) Effect of drought stress on metabolite adjustments in drought tolerant and sensitive thyme. Plant Physiol Biochem 132:391–399. https://doi.org/10.1016/j.plaphy.2018.09.009
Aslam MN, Nelson MN, Kailis SG, Bayliss KL, Speijers J, Cowling WA (2009) Canola oil increases in polyunsaturated fatty acids and decreases in oleic acid in drought-stressed Mediterranean-type environments. Plant Breed 128(4):348–355
Baghbani-Arani A, Modarres-Sanavy SAM, Mashhadi-Akbar-Boojar M, Mokhtassi-Bidgoli A (2017) Towards improving the agronomic performance, chlorophyll fluorescence parameters and pigments in fenugreek using zeolite and vermicompost under deficit water stress. Ind Crop Prod 109:346–357. https://doi.org/10.1016/j.indcrop.2017.08.049
Bahar NH, Lo M, Sanjaya M, Van Vianen J, Alexander P, Ickowitz A, Sunderland T (2020) Meeting the food security challenge for nine billion people in 2050: What impact on forests. Glob Environ Chang 62:102056. https://doi.org/10.1016/j.gloenvcha.2020.102056
Colonna E, Rouphael Y, Barbieri G, De Pascale S (2016) Nutritional quality of ten leafy vegetables harvested at two light intensities. Food Chem 199:702–710. https://doi.org/10.1016/j.foodchem.2015.12.068
Fahad S, Nie L, Chen Y, Wu C, Xiong D, Saud S, Huang J (2015) Crop plant hormones and environmental stress. Sustain Agric Rev. https://doi.org/10.1007/978-3-319-09132-7_10
Fard NS, Abad HHS, Rad AS, Heravan EM, Daneshian J (2018) Effect of drought stress on qualitative characteristics of canola cultivars in winter cultivation. Ind Crop Prod 114:87–92. https://doi.org/10.1016/j.indcrop.2018.01.082
Feizabadi A, Noormohammadi G, Fatehi F (2021) Changes in growth, physiology, and fatty acid profile of rapeseed cultivars treated with vermicompost under drought stress. J Soil Sci Plant Nutr 21(1):200–208. https://doi.org/10.1007/s42729-020-00353-4
Finkemeier I, Sweetlove LJ (2009) The role of malate in plant homeostasis. F1000 Biology Reports, 1. https://doi.org/10.3410/B1-47
Gai Z, Wang Y, Ding Y, Qian W, Qiu C, Xie H, Ding Z (2020) Exogenous abscisic acid induces the lipid and flavonoid metabolism of tea plants under drought stress. Sci Rep 10(1):1–13. https://doi.org/10.1038/s41598-020-69080-1
Ghasemzadeh N, Iranbakhsh A, Oraghi-Ardebili Z, Saadatmand S, & Jahanbakhsh-Godehkahriz S (2022) Cold plasma can alleviate cadmium stress by optimizing growth and yield of wheat (Triticum aestivum L.) through changes in physio-biochemical properties and fatty acid profile. Environ Sci Pollution Res, 1–11. https://doi.org/10.1007/s11356-022-18630-3
Gujjar RS, Supaibulwatana K (2019) The mode of cytokinin functions assisting plant adaptations to osmotic stresses. Plants 8(12):542. https://doi.org/10.3390/plants8120542
Gujjar RS, Banyen P, Chuekong W, Worakan P, Roytrakul S, Supaibulwatana K (2020) A Synthetic Cytokinin Improves photosynthesis in rice under drought stress by modulating the abundance of proteins related to stomatal conductance, chlorophyll contents, and rubisco activity. Plants 9(9):1106. https://doi.org/10.3390/plants9091106
Hai NN, Chuong NN, Tu NHC, Kisiala A, Hoang XLT, Thao NP (2020) Role and regulation of cytokinins in plant response to drought stress. Plants 9(4):422. https://doi.org/10.3390/plants9040422
He M, Qin CX, Wang X, Ding NZ (2020) Plant unsaturated fatty acids: Biosynthesis and regulation. Front Plant Sci 390:1-13
Hlahla JM, Mafa MS, Van der Merwe R, Alexander O, Duvenhage MM, Kemp, G, Moloi MJ (2022) The photosynthetic efficiency and carbohydrates responses of six edamame (Glycine max. L. Merrill) cultivars under drought stress. Plants, 11(3): 394. https://doi.org/10.3390/plants11030394
Huang X, Hou L, Meng J, You H, Li Z, Gong Z, Shi Y (2018) The antagonistic action of abscisic acid and cytokinin signaling mediates drought stress response in Arabidopsis. Molecul Plants 11(7):970–982. https://doi.org/10.1016/j.molp.2018.05.001
Janećková H (2021) Effects of Aromatic Cytokinins on Senescence-Induced Alterations in Photosynthesis. Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture, 71–84
Khazaei Z, Esmaielpour B, Estaji A (2020) Ameliorative effects of ascorbic acid on tolerance to drought stress on pepper (Capsicum annuum L.) plants. Physiol Molecul Biol Plant 26(8):1649–1662. https://doi.org/10.1007/s12298-020-00846-7
Kumari S, Kumar S, Prakash P (2018) Exogenous application of cytokinin (6-BAP) ameliorates the adverse effect of combined drought and high temperature stress in wheat seedling. J Pharm Phytochem 7(1):1176–1180
Li SM, Zheng HX, Zhang XS, Sui N (2020a) Cytokinins as central regulators during plant growth and stress response. Plant Cell Rep. https://doi.org/10.1007/s00299-020-02612-1
Li X, Li S, Wang J, Lin J (2020b) Exogenous abscisic acid alleviates harmful effect of salt and alkali stresses on wheat seedlings. Int J Environ Res Public Health 17(11):3770. https://doi.org/10.3390/ijerph17113770
Liang B, Gao T, Zhao Q, Ma C, Chen Q, Wei Z, Ma F (2018) Effects of exogenous dopamine on the uptake, transport, and resorption of apple ionome under moderate drought. Front Plant Sci 9:755. https://doi.org/10.3389/fpls.2018.00755
Liu F, Xing S, Ma H, Du Z, Ma B (2013) Cytokinin-producing, plant growth-promoting rhizobacteria that confer resistance to drought stress in Platycladus orientalis container seedlings. Appl Microbiol Biotechnol 97(20):9155–9164. https://doi.org/10.1007/s00253-013-5193-2
Liu C, Zhao X, Yan J, Yuan Z, Gu M (2019) Effects of salt stress on growth, photosynthesis, and mineral nutrients of 18 pomegranate (Punica granatum) cultivars. Agronomy 10(1):27. https://doi.org/10.3390/agronomy10010027
Ma D, Sun D, Wang C, Ding H, Qin H, Hou J, Guo T (2017) Physiological responses and yield of wheat plants in zinc-mediated alleviation of drought stress. Front Plant Sci 8:860. https://doi.org/10.3389/fpls.2017.00860
Ma X, Zhang J, Burgess P, Rossi S, Huang B (2018) Interactive effects of melatonin and cytokinin on alleviating drought-induced leaf senescence in creeping bentgrass (Agrostis stolonifera). Environ Exp Bot 145:1–11. https://doi.org/10.1016/j.envexpbot.2017.10.010
Márquez G, Alarcón MV, Salguero J (2019) Cytokinin inhibits lateral root development at the earliest stages of lateral root primordium initiation in maize primary root. J Plant Growth Regul 38(1):83–92. https://doi.org/10.1007/s00344-018-9811-1
Mickky B, Aldesuquy H, Elnajar M (2020) Effect of drought on yield of ten wheat cultivars linked with their flag leaf water status, fatty acid profile and shoot vigor at heading. Physiol Molecul Biol Plants 26(6):1111. https://doi.org/10.1007/s12298-020-00807-0
Nakashima K, Yamaguchi-Shinozaki K, Shinozaki K (2014) The transcriptional regulatory network in the drought response and its crosstalk in abiotic stress responses including drought, cold, and heat. Front Plant Sci 5:170. https://doi.org/10.3389/fpls.2014.00170
Nasirzadeh L, Sorkhilaleloo B, Hervan EM, Fatehi F (2021) Changes in antioxidant enzyme activities and gene expression profiles under drought stress in tolerant, intermediate, and susceptible wheat genotypes. Cereal Res Commun 49(1):83–89. https://doi.org/10.1007/s42976-020-00085-2
Nasirzadeh L, Kvarnheden A, Sorkhilaleloo B, Hervan EM, Fatehi F (2022) Foliar-Applied Selenium Nanoparticles Can Alleviate Soil-Cadmium Stress Through Physio-chemical and Stomatal Changes to Optimize Yield, Antioxidant Capacity, and Fatty Acid Profile of Wheat (Triticum aestivum L.). J Soil Sci Plant Nutr 1–12. https://doi.org/10.1007/s42729-022-00821-z
Noman A, Ali Q, Maqsood J, Iqbal N, Javed MT, Rasool N, Naseem J (2018) Deciphering physio-biochemical, yield, and nutritional quality attributes of water-stressed radish (Raphanus sativus L.) plants grown from Zn-Lys primed seeds. Chemosphere 195:175–189. https://doi.org/10.1016/j.chemosphere.2017.12.059
Phillips K, Ludidi N (2017) Drought and exogenous abscisic acid alter hydrogen peroxide accumulation and differentially regulate the expression of two maize RD22-like genes. Sci Report 7(1):1–12. https://doi.org/10.1038/s41598-017-08976-x
Rodríguez LH, Morales DA, Romero CD, Rodríguez ER (2012) The organic acid profile in wheat cultivar grains. Int J Food Sci Technol 47(3):627–632. https://doi.org/10.1111/j.1365-2621.2011.02886.x
Sánchez-Martín J, Canales FJ, Tweed JK, Lee MR, Rubiales D, Gómez-Cadenas A, Prats E (2018) Fatty acid profile changes during gradual soil water depletion in oats suggests a role for jasmonates in coping with drought. Front Plant Sci 9:1077. https://doi.org/10.3389/fpls.2018.01077
Sarker U, Oba S (2018) Response of nutrients, minerals, antioxidant leaf pigments, vitamins, polyphenol, flavonoid and antioxidant activity in selected vegetable amaranth under four soil water content. Food Chem 252:72–83. https://doi.org/10.1016/j.foodchem.2018.01.097
Shafiq S, Akram NA, Ashraf M, Arshad A (2014) Synergistic effects of drought and ascorbic acid on growth, mineral nutrients and oxidative defense system in canola (Brassica napus L.) plants. Acta Physiol Plant 36(6):1539–1553. https://doi.org/10.1007/s11738-014-1530-z
Somasundaram S, Jeong J, Irisappan G, Kim TW, Hong SH (2020) Enhanced production of malic acid by co-localization of phosphoenolpyruvate carboxylase and malate dehydrogenase using synthetic protein scaffold in Escherichia coli. Biotechnol Bioproc Engin 25(1):32–45
Teixeira NC, Valim JOS, Oliveira MGA, Campos WG (2020) Combined effects of soil silicon and drought stress on host plant chemical and ultrastructural quality for leaf-chewing and sap-sucking insects. J Agronomy Crop Sci 206(2):187–201. https://doi.org/10.1111/jac.12386
Verma V, Ravindran P, Kumar P (2016) Plant hormone-mediated regulation of stress responses. BMC Plant Biol 16(1):1–10. https://doi.org/10.1186/s12870-016-0771-y
Wang X, Mao Z, Zhang J, Hemat M, Huang M, Cai J, Jiang D (2019) Osmolyte accumulation plays important roles in the drought priming induced tolerance to post-anthesis drought stress in winter wheat (Triticum aestivum L.). Environ Exp Bot 166:103804
Wang X, Li Q, Xie J, Huang M, Cai J, Zhou Q, Jiang D (2021) Abscisic acid and jasmonic acid are involved in drought priming-induced tolerance to drought in wheat. Crop J 9(1):120–132. https://doi.org/10.1016/j.cj.2020.06.002
Yang J, Zhang J, Wang Z, Zhu Q (2003) Hormones in the grains in relation to sink strength and postanthesis development of spikelets in rice. Plant Growth Regul 41(3):185–195
Yang D, Luo Y, Kong X, Huang C, Wang Z (2021) Interactions between exogenous cytokinin and nitrogen application regulate tiller bud growth via sucrose and nitrogen allocation in winter wheat. J Plant Growth Regul 40(1):329–341. https://doi.org/10.1007/s00344-020-10106-3
Yudina L, Sukhova E, Sherstneva O, Grinberg M, Ladeynova M, Vodeneev V, Sukhov V (2020) Exogenous abscisic acid can influence photosynthetic processes in peas through a decrease in activity of H+-ATP-ase in the plasma membrane. Biology 9(10):324. https://doi.org/10.3390/biology9100324
Zafar-ul-Hye M, Danish S, Abbas M, Ahmad M, Munir TM (2019) ACC deaminase producing PGPR Bacillus amyloliquefaciens and Agrobacterium fabrum along with biochar improve wheat productivity under drought stress. Agronom 9(7):343. https://doi.org/10.3390/agronomy9070343
Zhou Y, He W, Zheng W, Tan Q, Xie Z, Zheng C, Hu C (2018) Fruit sugar and organic acid were significantly related to fruit Mg of six citrus cultivars. Food Chem 259:278–285. https://doi.org/10.1016/j.foodchem.2018.03.102
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Khosravi-nejad, F., Khavari-nejad, R.A., Moradi, F. et al. Cytokinin and abscisic acid alleviate drought stress through changing organic acids profile, ion immolation, and fatty acid profile to improve yield of wheat (Triticum aestivum L.) cultivars. Physiol Mol Biol Plants 28, 1119–1129 (2022). https://doi.org/10.1007/s12298-022-01173-9
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DOI: https://doi.org/10.1007/s12298-022-01173-9