Abstract
Sulforaphane (SF) is one of the most effective natural products in preventing and fighting cancer, found in cruciferous plants. In this study, broccoli hairy roots grown for 20 d were used as the experimental material, and it was treated with 500 μmol/L melatonin (MT) for 0, 12 and 32 h to explore the effect of MT on the conversion of glucoraphanin (GRA) to SF. Results showed that the yields of GRA and SF were the largest under MT treatment for 12 h, which were 1.53 and 1.93-fold, respectively, compared to 0 h. However, Myrosinases activity was the highest under MT treatment for 32 h, which was 1.42-fold compared to that of the 0 h. The differential expression of key genes involved in GRA conversion to SF in broccoli hairy roots was identified transcriptome sequencing, and the path of the transformation from GRA to SF was simulated, which provided a theoretical basis for establishing an efficient transformation system from GRA to SF.
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Data availability
The sequencing raw data has been uploaded to the Sequence Read Archive (https://www.ncbi.nlm.nih.gov/sra) under Bioproject PRJNA764317.
Abbreviations
- GSLs:
-
Glucosinolates
- GRA:
-
Glucoraphanin
- SF:
-
Sulforaphane
- MT:
-
Melatonin
- DEGs:
-
Differentially Expressed Genes
- ESP:
-
Epithiospecifier Protein
- NSP:
-
Nitrile-specific Proteins
- MYR:
-
Myrosinase
- qRT-PCR:
-
Quantitative real-time polymerase chain reaction
- COG:
-
Cluster of Orthologous Groups of proteins
- GO:
-
Gene Ontology
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (31860067), Gansu Province Higher Education Innovation Fund Project (2021B-136), Gansu Agricultural University Patent Transformation Project (GSAU-JSZR-2021-001), Longyuan Youth Innovation and Entrepreneurship Project (2016-3-18), Gansu Provincial People’s Livelihood Science and Technology Project (1603FCMG007).
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XLZ designed the experiments and wrote the manuscript. XLZ, PT and JY performed the experiments. XLZ, JYB and YCW analyzed the data. SL, SYM, XL and JYB revised the manuscript. All authors read and approved the final manuscript.
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Zhang, X., Bao, J., Lu, X. et al. Transcriptome analysis of melatonin regulating the transformation of glucoraphanin to sulforaphane in broccoli hairy roots. Physiol Mol Biol Plants 28, 51–64 (2022). https://doi.org/10.1007/s12298-022-01143-1
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DOI: https://doi.org/10.1007/s12298-022-01143-1