Abstract
Chlorosarcinopsis eremi is a member of Chlamydomonadales algae which is isolated from terrestrial environments. In this study, the mitochondrial genome of C. eremi isolated from desert region of Iran, was represented for the first time. Following sequencing, assembly and annotation, comparative analyses of C. eremi and other available Chlamydomonadales algae complete mitochondrial genomes were performed. The mitochondrial genome of C. eremi was circular, had a low number of genes coding in the same strand with a minor amount of repeated sequences; same as other non-Reinhardtinia species of Chlamydomonadales algae. GC content of C. eremi mitochondrial genome was in normal range when compared with non-Chlamydomonadales organisms, but among Chlamydomonadales algae, C. eremi had a low GC content mitochondrial genome. C. eremi had the highest percent of non-coding sequences in comparison with other available Chlamydomonadales mitochondrial genomes which was related to intergenic regions. Identity analysis of protein-coding sequences of Chlamydomonadales mitochondrial genomes showed more divergences and may be related to the high mutation rate of mitochondrial genome reported in microbial eukaryotes.
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Acknowledgements
The authors would like to thank Dr Sayed-Amir Marashi for his cooperation to provide his Systems Biology laboratory’s facilities. This project was carried out at Shayesteh Sepehr Laboratories and funded by Vice Chancellor of Alzahra University.
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Parisa Mohammadi was responsible for the study concept. Acquisition of data including DNA extraction and bioinformatics analysis was done by Fatemeh Khani-Juyabad. The interpretation of data was implemented by Fatemeh Khani-Juyabad, Parisa Mohammadi and Mahbubeh Zarrabi. Fatemeh Khani-Juyabad prepared the draft of the manuscript. The critical revision of the manuscript for important intellectual content as well as supervision of the study was performed by Parisa Mohammadi and Mahbubeh Zarrabi. Administrative and material support was provided by Parisa Mohammadi.
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Khani-Juyabad, F., Mohammadi, P. & Zarrabi, M. Comparative analysis of Chlorosarcinopsis eremi mitochondrial genome with some Chlamydomonadales algae. Physiol Mol Biol Plants 25, 1301–1310 (2019). https://doi.org/10.1007/s12298-019-00696-y
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DOI: https://doi.org/10.1007/s12298-019-00696-y