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Impact of cultivation factors in vitro on the growth and the biosynthesis of steviol glycosides in Stevia rebaudiana cell cultures

Abstract

The growth and the biosynthesis of steviol glycosides (SGs) in Stevia rebaudiana cell cultures subjected to the impact of different cultivation factors were investigated under in vitro conditions. The changes in composition and concentration of constituted components of the nutrient medium was found to exert a significant influence on the of cell culture growth and the biosynthesis of SGs. It was found that combination of growth regulators in the nutrient medium is more important factor for proliferation of isolated cells of S. rebaudiana while the level of SGs accumulation depends to greater extent on genotypical features of cell strain. Light was shown to activate the biosynthesis of SGs, providing evidence for the suggestion about chloroplasts participation in this activation. In morphogenic callus cultures, the content of SGs was about ten times higher, indicating that the level of the accumulation of the compounds in question depends on cell differentiation and specialization.

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Abbreviations

BAP:

6-Benzylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

HPLC:

High performance liquid chromatography

IAA:

Indole-3-acetic acid

IBA:

Iindole-3-butyric acid

MS:

Murashige and Skoog

NAA:

1-Naphthalene acetic acid

SGs:

Steviol glycosides

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Acknowledgements

This work was supported by the Russian Research Fund [Number 14-50-00029 «Scientific principles for creation of National Depository Bank for Live Systems. Direction “Plants”»].

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NB and TB carried out the in vitro experiments and the extraction of steviol glycosides, analyzed the data and wrote the manuscript. OR and MI developed the analytical method for determination of steviol glycosides and analyzed HPLC data. AN edited the version for publishing. All authors read and approved the manuscript.

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Correspondence to Nikolai Bondarev.

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Bondarev, N., Reshetnyak, O., Bondareva, T. et al. Impact of cultivation factors in vitro on the growth and the biosynthesis of steviol glycosides in Stevia rebaudiana cell cultures. Physiol Mol Biol Plants 25, 1091–1096 (2019). https://doi.org/10.1007/s12298-019-00680-6

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Keywords

  • Cell culture
  • Steviol glycosides
  • Cultivation factors
  • In vitro
  • Stevia rebaudiana