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Antimicrobial and cytotoxic activity of extracts from Salvia tebesana Bunge and Salvia sclareopsis Bornm cultivated in Iran


Salvia, a member of the Lamiaceae family, represents more than 58 species in Iran. In the present study, antibacterial and cytotoxic activity of extracts obtained from the roots of Salvia tebesana and Salvia sclareopsis were investigated. The antibacterial activity of the extracts was investigated against 4 bacterial strains and yeast using serial dilution method. The petroleum ether and CH2Cl2 extracts of S. tebesana showed a good activity against Gram-positive bacteria particularly Bacillus cereus (MIC 1.25 mg/mL) while Gram-negative bacteria and yeast were resistant to the extracts. Also, the cytotoxic effects of the extracts on A2780 (ovarian), MCF-7 (breast) and DU 145 (prostate) cancer cell lines were examined using AlamarBlue® assay. The petroleum ether and CH2Cl2 extracts of S. tebesana were found to be cytotoxic against the tested cell lines, with IC50 values less than 50 µg/mL. The petroleum ether extract also showed a potent anti-proliferative activity against DU 145 cells with the lowest IC50 value (6.25 µg/mL).

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Dimethyl sulfoxide


Minimum inhibitory concentration


Minimum bactericidal concentration


Muller Hinton broth


2,3,5-Triphenyltetrazolium chloride


Reactive oxygen species


Dichloro-dihydro-fluorescein diacetate


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This work was supported by Research Affairs of Mashhad University of Medical Sciences.

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S. Eghbaliferiz, V. Soheili, S.A. Emami, M. Iranshahi and Z. Tayarani-Najaran conducted experiments and analyzed data. S. Eghbaliferiz wrote the manuscript. All authors read and approved the manuscript.

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Correspondence to Javad Asili.

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Eghbaliferiz, S., Soheili, V., Tayarani-Najaran, Z. et al. Antimicrobial and cytotoxic activity of extracts from Salvia tebesana Bunge and Salvia sclareopsis Bornm cultivated in Iran. Physiol Mol Biol Plants 25, 1083–1089 (2019).

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  • Salvia tebesana
  • Salvia sclareopsis
  • Diterpenoids
  • Antibacterial activity
  • Cytotoxic assay