Skip to main content

Antimicrobial and cytotoxic activity of extracts from Salvia tebesana Bunge and Salvia sclareopsis Bornm cultivated in Iran

Abstract

Salvia, a member of the Lamiaceae family, represents more than 58 species in Iran. In the present study, antibacterial and cytotoxic activity of extracts obtained from the roots of Salvia tebesana and Salvia sclareopsis were investigated. The antibacterial activity of the extracts was investigated against 4 bacterial strains and yeast using serial dilution method. The petroleum ether and CH2Cl2 extracts of S. tebesana showed a good activity against Gram-positive bacteria particularly Bacillus cereus (MIC 1.25 mg/mL) while Gram-negative bacteria and yeast were resistant to the extracts. Also, the cytotoxic effects of the extracts on A2780 (ovarian), MCF-7 (breast) and DU 145 (prostate) cancer cell lines were examined using AlamarBlue® assay. The petroleum ether and CH2Cl2 extracts of S. tebesana were found to be cytotoxic against the tested cell lines, with IC50 values less than 50 µg/mL. The petroleum ether extract also showed a potent anti-proliferative activity against DU 145 cells with the lowest IC50 value (6.25 µg/mL).

This is a preview of subscription content, access via your institution.

Abbreviations

DMSO:

Dimethyl sulfoxide

MIC:

Minimum inhibitory concentration

MBC:

Minimum bactericidal concentration

MHB:

Muller Hinton broth

TTC:

2,3,5-Triphenyltetrazolium chloride

ROS:

Reactive oxygen species

DCFH-DA:

Dichloro-dihydro-fluorescein diacetate

DCF:

Discounted cash flow

References

  1. Alimpic AZ, Kotur N, Stanković B, Marin PD, Matevski V, Al Sheef N, Duletić-Laušević S (2015) The in vitro antioxidative and cytotoxic effects of selected Salvia species water extracts. J Appl Bot Food Qual 88:1–9

    Google Scholar 

  2. Asili J, Emami SA, Rahimizadeh M, Fazly-Bazzaz BS, Hassanzadeh MK (2008) Chemical and antimicrobial studies of Juniperus excelsa subsp. excelsa and Juniperus excelsa subsp. polycarpos essential oils. J Essent Oil Bear Plants 11:292–302

    Article  CAS  Google Scholar 

  3. Askun T, Tumen G, Satil F, Ates M (2009) Characterization of the phenolic composition and antimicrobial activities of Turkish medicinal plants. Pharm Biol 47:563–571

    Article  CAS  Google Scholar 

  4. Bézivin C, Tomasi S, Lohézic-Le Dévéhat F, Boustie J (2003) Cytotoxic activity of some lichen extracts on murine and human cancer cell lines. Phytomedicine 10:499–503

    Article  PubMed  Google Scholar 

  5. Bisio A, De M, Maria M, Luigi S, Anna M, Anita R, Daniela A, Silvana L, Margherita T, Hamburger M (2017) Antibacterial and hypoglycemic diterpenoids from Salvia Chamaedryoides. J Nat Prod 80:503–514

    Article  CAS  PubMed  Google Scholar 

  6. Duletić-Laušević S, Aradski AA, Šavikin K, Knežević A, Milutinović M, Stević T, Vukojević J, Marković S, Marin P (2018) Composition and biological activities of Libyan Salvia fruticosa Mill. and S. lanigera Poir. extracts. S Afr J Bot 117:101–109

    Article  CAS  Google Scholar 

  7. Eghbaliferiz S, Emami SA, Tayarani-Najaran Z, Iranshahi M, Shakeri A, Hohmann J, Asili J (2018) Cytotoxic diterpene quinones from Salvia tebesana Bunge. Fitoterapia 128:97–101

    Article  CAS  PubMed  Google Scholar 

  8. Elson CE (1995) Suppression of mevalonate pathway activities by dietary isoprenoids: protective roles in cancer and cardiovascular disease. J Nutr 125:1666S–1672S

    CAS  PubMed  Google Scholar 

  9. Goldansaz SM, Meybodi MHH, Mirhosseini A, Mirjalili MH (2017) Essential oil composition of Salvia tebesana Bunge (Lamiaceae) from Iran. Rec Nat Prod 11:9

    Google Scholar 

  10. Hamidpour M, Hamidpour R, Hamidpour S, Shahlari M (2014) Chemistry, pharmacology, and medicinal property of sage (Salvia) to prevent and cure illnesses such as obesity, diabetes, depression, dementia, lupus, autism, heart disease, and cancer. J Tradit Complement Med 4:82–88

    Article  PubMed  PubMed Central  Google Scholar 

  11. Hawas UW, El-Ansari MA (2006) Flavonoids and antimicrobial activity of Salvia lanigera Poir. JASMR 1:159–167

    Google Scholar 

  12. Jamzad M, Rustaiyan A, Masoudi S, Jamzad Z, Yari M (2009) Volatile constituents of three Salvia species: Salvia sclareopsis Bornm. ex Hedge., Salvia brachysiphon Stapf and Salvia verbascifolia M. Bieb. growing wild in Iran. J Essent Oil Res 21:19–21

    Article  CAS  Google Scholar 

  13. Jeong J, Cha H, Choi E, Kim C, Kim G, Yoo Y, Hwang H, Park H, Yoon H, Choi Y (2019) Activation of the Nrf2/HO-1 signaling pathway contributes to the protective effects of baicalein against oxidative stress-induced DNA damage and apoptosis in HEI193 Schwann cells. Int J Med Sci 16:145–155

    Article  PubMed  PubMed Central  Google Scholar 

  14. Khan A, AlKharfy KM, Gilani A-H (2011) Antidiarrheal and antispasmodic activities of Salvia officinalis are mediated through activation of K + channels. Bangladesh J Pharmacol 6:111–116

    Google Scholar 

  15. Moradi-Afrapoli F, Shokrzadeh M, Barzegar F, Gorji-Bahri G, Zadali R, Ebrahimi SN (2018) Cytotoxic activity of abietane diterpenoids from roots of Salvia sahendica by HPLC-based activity profiling. Rev Bras Farmacogn 28:27–33

    Article  CAS  Google Scholar 

  16. O’brien J, Wilson I, Orton T, Pognan F (2000) Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. FEBS J 267:5421–5426

    Google Scholar 

  17. Oliveira CS, Salvador MJ, de Carvalho JE, Barison A (2016) Cytotoxic abietane-derivative diterpenoids of Salvia lachnostachys. Phytochem Lett 17:140–143

    Article  CAS  Google Scholar 

  18. Otsuka H (2006) Purification by solvent extraction using partition coefficient. Natural products isolation. Springer, Berlin, pp 269–273

    Google Scholar 

  19. Pavić V, Jakovljević M, Molnar M, Jokić S (2019) Extraction of carnosic acid and carnosol from sage (Salvia officinalis L.) leaves by supercritical fluid extraction and their antioxidant and antibacterial activity. Plants 8:16

    Article  PubMed Central  Google Scholar 

  20. SalarBashi D, Fazly Bazzaz BS, Sahebkar A, Karimkhani MM, Ahmadi A (2012) Investigation of optimal extraction, antioxidant, and antimicrobial activities of Achillea biebersteinii and A. wilhelmsii. Pharm Biol 50:1168–1176

    Article  Google Scholar 

  21. Salarbashi D, Dowom SA, Bazzaz BSF, Khanzadeh F, Soheili V, Mohammadpour A (2016) Evaluation, prediction and optimization the ultrasound-assisted extraction method using response surface methodology: antioxidant and biological properties of Stachys parviflora L. Iran J Basic Med Sci 19:529

    Google Scholar 

  22. Salari S, Bakhshi T, Sharififar F, Naseri A, Almani PGN (2016) Evaluation of antifungal activity of standardized extract of Salvia rhytidea Benth. (Lamiaceae) against various Candida isolates. J Mycol Med 26:323–330

    Article  CAS  PubMed  Google Scholar 

  23. Shaheen UY, Hussain MH, Ammar HA (2011) Cytotoxicity and antioxidant activity of new biologically active constituents from Salvia lanigra and Salvia splendens. Pharmacogn J 3:36–48

    Article  CAS  Google Scholar 

  24. Shakeri A, Khakdan F, Soheili V, Sahebkar A, Rassam G, Asili J (2014) Chemical composition, antibacterial activity, and cytotoxicity of essential oil from Nepeta ucrainica L. spp. kopetdaghensis. Ind Crops Prod 58:315–321

    Article  CAS  Google Scholar 

  25. Shakeri A, Khakdan F, Soheili V, Sahebkar A, Shaddel R, Asili J (2016) Volatile composition, antimicrobial, cytotoxic and antioxidant evaluation of the essential oil from Nepeta sintenisii Bornm. Ind Crops Prod 84:224–229

    Article  CAS  Google Scholar 

  26. Shakeri A, Akhtari J, Soheili V, Taghizadeh SF, Sahebkar A, Shaddel R, Asili J (2017) Identification and biological activity of the volatile compounds of Glycyrrhiza triphylla Fisch. & C.A.Mey. Microb Pathog 109:39–44

    Article  CAS  PubMed  Google Scholar 

  27. Shakeri A, Sharifi MJ, Fazly Bazzaz BS, Emami A, Soheili V, Sahebkar A, Asili J (2018a) Bioautography detection of antimicrobial compounds from the essential oil of Salvia pachystachys. Curr Bioact Compd 14:80–85

    Article  CAS  Google Scholar 

  28. Shakeri A, Soheili V, Karimi M, Hosseininia SA, Bazzaz BSF (2018b) Biological activities of three natural plant pigments and their health benefits. J Food Meas Charact 12:356–361

    Article  Google Scholar 

  29. Sharma OP, Bhat TK (2009) DPPH antioxidant assay revisited. Food Chem 113:1202–1205

    Article  CAS  Google Scholar 

  30. Soltanian H, Rezaeian S, Shakeri A, Janpoor J, Pourianfar HR (2016) Antibacterial activity of crude extracts and fractions from Iranian wild-grown and cultivated Agaricus spp. Biomed Res 27:56–59

    CAS  Google Scholar 

  31. Szychowski KA, Binduga UE, Rybczyńska-Tkaczyk K, Leja ML, Gmiński J (2018) Cytotoxic effects of two extracts from garlic (Allium sativum L.) cultivars on the human squamous carcinoma cell line SCC-15. Saudi J Biol Sci 25(8):1703–1712

    Article  CAS  PubMed  Google Scholar 

  32. Taran M, Ghasempour Hamid R, Borzo S, Najafi S, Samadian E (2011) In vitro antibacterial and antifungal activity of Salvia multicaulis. J Essent Oil Bear Plants 14:255–259

    Article  Google Scholar 

  33. Walch SG, Ngaba Tinzoh L, Zimmermann BF, Stühlinger W, Lachenmeier DW (2011) Antioxidant capacity and polyphenolic composition as quality indicators for aqueous infusions of Salvia officinalis L. (sage tea). Front Pharmacol 2:79

    Article  PubMed  PubMed Central  Google Scholar 

  34. Wu C-Y, Liao Y, Yang Z-G, Yang X-W, Shen X-L, Li R-T, Xu G (2014) Cytotoxic diterpenoids from Salvia yunnanensis. Phytochemistry 106:171–177

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by Research Affairs of Mashhad University of Medical Sciences.

Author information

Affiliations

Authors

Contributions

S. Eghbaliferiz, V. Soheili, S.A. Emami, M. Iranshahi and Z. Tayarani-Najaran conducted experiments and analyzed data. S. Eghbaliferiz wrote the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Javad Asili.

Ethics declarations

Conflict of interest

The authors declare that there is no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Eghbaliferiz, S., Soheili, V., Tayarani-Najaran, Z. et al. Antimicrobial and cytotoxic activity of extracts from Salvia tebesana Bunge and Salvia sclareopsis Bornm cultivated in Iran. Physiol Mol Biol Plants 25, 1083–1089 (2019). https://doi.org/10.1007/s12298-019-00652-w

Download citation

Keywords

  • Salvia tebesana
  • Salvia sclareopsis
  • Diterpenoids
  • Antibacterial activity
  • Cytotoxic assay