Physiology and Molecular Biology of Plants

, Volume 25, Issue 3, pp 799–805 | Cite as

Genetic characterization of Portuguese allochthonous populations of Pinus nigra using ISSRs and SCoTs and extrapolation of their infraspecific taxonomy

  • Alexandra Dias
  • Maria Lemos
  • Ivo Pavia
  • Maria João Gaspar
  • Maria Emília Silva
  • José Luís Louzada
  • José Lima-Brito
  • Ana CarvalhoEmail author
Short Communication


The Pinus nigra distribution in Portugal is restricted to six allochthonous populations with unknown origin and infraspecific taxonomy. This work intends to evaluate their genetic diversity, structure and relationships, and to infer about their infraspecific taxonomy by comparing molecular patterns produced by inter-simple sequence repeat and Start Codon Targeted markers among Portuguese and foreign samples with known taxonomy and provenance. 127 Portuguese P. nigra individuals were clustered per population. The genetic differentiation was higher within rather than among populations. The pooled molecular data indicated high genetic proximity among the Portuguese and foreign samples of subspecies laricio. However, the separate analysis per marker system demonstrated that two varieties of subspecies laricio (corsicana and calabrica) may have been used in the plantations of the Portuguese P. nigra stands performed in the last century. The genetic characterization and extrapolation of the intraspecific taxonomy of these populations provide useful information for forest management, afforestation and germplasm use.


European black pine Genetic structure Infraspecific taxa Molecular markers 



The authors acknowledge to Prof. Bruno Fady (INRA, Avignon, France) and Dr. Marc Calvignac (Vilmorin S.A., La Ménitré, France) that kindly provided dehydrated needles and certified seeds, respectively, representative of different Pinus nigra taxa.


This study was funded by “Fundação para a Ciência e a Tecnologia” (FCT) (grant SFRH/BD/91781/2012 co-financed by “Fundo Social Europeu” under the POPH-QREN program and projects UID/AGR/04033/2019 (to CITAB/UTAD), UID/MULTI/04046/2019 (to BioISI), and POCI-01-0145-FEDER-006958 - FEDER/COMPETE/POCI – Operational Competitiveness and Internationalization Program (to CITAB/UTAD).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

12298_2019_649_MOESM1_ESM.pdf (114 kb)
Supplementary material 1 (PDF 114 kb)
12298_2019_649_MOESM2_ESM.pdf (11 kb)
Supplementary material 2 (PDF 11 kb)
12298_2019_649_MOESM3_ESM.pdf (65 kb)
Supplementary material 3 (PDF 65 kb)


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2019

Authors and Affiliations

  1. 1.Center of Research and Technology of Agroenvironmental and Biological Sciences (CITAB)University of Tras-os-Montes and Alto DouroVila RealPortugal
  2. 2.University of Tras-os-Montes and Alto DouroVila RealPortugal
  3. 3.BioISI - Biosystems & Integrative Sciences Institute, University of Tras-os-Montes and Alto Douro (BioISI/UTAD)Vila RealPortugal
  4. 4.Forest Research Centre (CEF), Instituto Superior de Agronomia (ISA)University of LisbonLisbonPortugal
  5. 5.Department of Genetics and Biotechnology (DGB), Ed. Blocos Laboratoriais, A1.09University of Tras-os-Montes and Alto DouroVila RealPortugal

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