Abstract
Herbaspirillum seropedicae is an endophytic diazotrophic bacterium and a plant growth promoting bacteria. Colletotrichum graminicola causes the anthracnose, one of the most destructive maize diseases worldwide. The main objective of this work was to evaluate the effects of H. seropedicae SmR1 strain on the plant growth and leaf anthracnose of maize plants grown in substrate amended or not amended with humic substances. In the first assay, plants were pre-treated with H. seropedicae and inoculated with C. graminicola at 7, 14 and 21 days after treatment (DAT). In the second assay, plants were treated with H. seropedicae, grown in substrate amended with humic substances and inoculated at 3 and 7 DAT. The anthracnose severity was assessed by measurement of necrotic and chlorotic leaf area, and bacteria were quantified in leaves by quantitative PCR. H. seropedicae did not affect the disease severity in maize leaves, although it efficiently colonized the leaf tissues and it promoted maize leaf growth. Humic substances improved H. seropedicae colonization in maize.
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Acknowledgements
This work was financially supported by the National Institute of Science and Technology-Biological Nitrogen Fixation (INCT-FBN), National Council for Scientific and Technological Development (CNPq), Ministry of Science and Technology, Brazil. The author would like to thank Professor Fábio Lopes Olivares, from University of North Fluminense, Brazil, who kindly provided the humic substances.
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This research is part of the doctoral thesis of Pâmela Dall’Asta. Aline C. Velho and Tomás P. Pereira contributed in the Colletotrichum graminicola and Herbaspirillum seropedicae practical procedures, respectively. Professors Ana Carolina M. Arisi and Marciel Stadnik are the supervisors. Pâmela Dall´Asta wrote the basic text and all the authors contributed by reading, revising and improving it, especially the professors Ana Carolina M. Arisi and Marciel Stadnik.
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Dall’Asta, P., Velho, A.C., Pereira, T.P. et al. Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose. Physiol Mol Biol Plants 25, 167–176 (2019). https://doi.org/10.1007/s12298-018-0616-2
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DOI: https://doi.org/10.1007/s12298-018-0616-2