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Physiology and Molecular Biology of Plants

, Volume 24, Issue 4, pp 631–641 | Cite as

Molecular insights into genetic diversity and population dynamics of five medicinal Eulophia species: a threatened orchid taxa of Africa

  • Paromik Bhattacharyya
  • Johannes van Staden
Research Article

Abstract

Genetic diversity existing amongst five Eulophia orchid species were assessed using start codon targeted polymorphism (SCoT) and inter-retrotransposon amplified polymorphism (IRAP) markers. A total of 12 SCoT and 5 IRAP markers revealed an average of 63% genetic variability [SCoT = 63.87; IRAP = 64.95%] amongst the five Eulophia species investigated. The genetic similarities were assessed using both UPGMA and Bayesian approaches which indicated identical clustering patterns at a genetic similarity level of 50%. Analysis of molecular variance (AMOVA) revealed the presence of a significant degree of genetic variability, mostly compartmentalized within the species level. Amongst the five assessed Eulophia species, E. parviflora was the most genetically diverse representative whereas E. welwitschii was found to be least diverse based on a comparative assessment of various population genetic parameters like Nei’s gene diversity (h) and Shannon’s information index (I) with an overall gene flow value greater than 1. In order to evaluate the comparative marker efficiency, SCoT and IRAP marker data were subjected to various benchmark analyses like marker index, resolving power, polymorphic index content, multiplex ratio and effective multiplex ratio which revealed the robustness of both the marker techniques in assessment of genetic diversity. The present report provides the first molecular insights into the aspects of inter and intra specific genetic variability in medicinally as well as horticulturally important Eulophia species along with addressing their conservation concerns. In a nutshell, the present approach is simple, rapid and cost effective and can be extended for analysis of genetic diversity of other related plant species.

Keywords

Gene targeted markers African medicinal orchids SCoT-PCR IRAP-PCR AMOVA 

Abbreviations

PCR

Polymerase chain reaction

PIC

Polymorphic information content

AMOVA

Analysis of molecular variance

Rp

Resolving power

Notes

Acknowledgements

Paromik Bhattacharyya thanks the University of KwaZulu-Natal (UKZN), South Africa for support in the form of a Postdoctoral Fellowship.

Supplementary material

12298_2018_523_MOESM1_ESM.doc (32 kb)
Supplementary material 1 (DOC 33 kb)

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  1. 1.Research Centre for Plant Growth and Development, School of Life SciencesUniversity of KwaZulu-Natal, PietermaritzburgScottsvilleSouth Africa

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