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Assessment of genetic diversity and population structure in gladiolus (Gladiolus hybridus Hort.) by ISSR markers

Abstract

ISSR (Inter simple sequence repeat) markers were used to assess the genetic diversity and population structure in 53 indigenous and exotic genotypes of gladiolus (Gladiolus hybridus Hort.). Molecular markers analysis showed PIC ranges from 0.42 (ISSR 861) to 0.99 (ISSR 855, ISSR 856 and ISSR 889) with an average 0.812, marker index ranged from 0.99 (ISSR 889) to 9.26 (ISSR 851) with an average 4.66 and resolving power of the primers ranged from 0.03 (ISSR 889) to 11.58 (ISSR 861) with an average value 3.80. The dendrogram based UPGMA clustering showed that all the 53 genotypes grouped into three main clusters. Nei’s gene diversity (Na) varied from 0.929 to 1.717, effective number of alleles (Ne) varied from 1.262 to 1.369, Shannon’s information index (I) ranged from 0.251 to 0.359 and gene diversity (He) was in the range from 0.167 to 0.229. Population structure analysis revealed three groups in which 32 genotypes were admixture types.

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Acknowledgements

The authors are grateful to Prf. Gaya Prasad, Hon’ble Vice Chancellor of the Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut, U.P. India for providing facilities and encouragement. Also, IIHR, Bangalore, PAU, Ludhiana, NBRI, Lucknow and IARI, New Delhi deserves thanks for providing gladiolus germplasm.

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Correspondence to Veena Chaudhary or Shailendra Sharma.

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Chaudhary, V., Kumar, M., Sharma, S. et al. Assessment of genetic diversity and population structure in gladiolus (Gladiolus hybridus Hort.) by ISSR markers. Physiol Mol Biol Plants 24, 493–501 (2018). https://doi.org/10.1007/s12298-018-0519-2

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Keywords

  • Gladiolus
  • Molecular diversity
  • Inter simple sequence repeats
  • Population structure