Assessment of genetic diversity and population structure in gladiolus (Gladiolus hybridus Hort.) by ISSR markers

  • Veena Chaudhary
  • Mukesh Kumar
  • Shailendra Sharma
  • Navneet Kumar
  • Vipin Kumar
  • Hemant Kumar Yadav
  • Shiveta Sharma
  • Ujjwal Sirohi
Research Article
  • 18 Downloads

Abstract

ISSR (Inter simple sequence repeat) markers were used to assess the genetic diversity and population structure in 53 indigenous and exotic genotypes of gladiolus (Gladiolus hybridus Hort.). Molecular markers analysis showed PIC ranges from 0.42 (ISSR 861) to 0.99 (ISSR 855, ISSR 856 and ISSR 889) with an average 0.812, marker index ranged from 0.99 (ISSR 889) to 9.26 (ISSR 851) with an average 4.66 and resolving power of the primers ranged from 0.03 (ISSR 889) to 11.58 (ISSR 861) with an average value 3.80. The dendrogram based UPGMA clustering showed that all the 53 genotypes grouped into three main clusters. Nei’s gene diversity (Na) varied from 0.929 to 1.717, effective number of alleles (Ne) varied from 1.262 to 1.369, Shannon’s information index (I) ranged from 0.251 to 0.359 and gene diversity (He) was in the range from 0.167 to 0.229. Population structure analysis revealed three groups in which 32 genotypes were admixture types.

Keywords

Gladiolus Molecular diversity Inter simple sequence repeats Population structure 

Notes

Acknowledgements

The authors are grateful to Prf. Gaya Prasad, Hon’ble Vice Chancellor of the Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut, U.P. India for providing facilities and encouragement. Also, IIHR, Bangalore, PAU, Ludhiana, NBRI, Lucknow and IARI, New Delhi deserves thanks for providing gladiolus germplasm.

References

  1. Anderson NO, Younis A, Sun Y (2010) Inter simple sequence repeats distinguish genetic differences in easter lily ‘Nellie White’ clonal ramets within and among bulb growers over years. J Am Soc Hortic Sci 135(5):445–455Google Scholar
  2. Banard TT (1972) On hybrid and hybridization. In: Lewis GJ, Obermeyer AA, Barnard TT (eds) Gladiolus a revision of the south african species. J of South Afric Bot, Supplement, 10: 304–310Google Scholar
  3. Bhajantri A, Patil VS (2013) Studies on ethyl methane sulphonate (EMS) induced mutations for enhancing variability of gladiolus varieties (Gladiolus hybridus Hort.) in M1V2 generation. Karnataka J Agric Sci 26:403–407Google Scholar
  4. Bornet B, Branchard M (2001) Non-anchored inter simple sequence repeat (ISSR) markers reproducible and specific tool for genome fingerprinting. Plant Mol Biol Rep 22:427–432Google Scholar
  5. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331PubMedPubMedCentralGoogle Scholar
  6. Cantor M, Chis LM (2009) New gladiolus cultivars homologated at USAMV Cluj-Napoca. Bull UASVM Hortic 66:504–509Google Scholar
  7. Cui GF, Wu LF, Wang XN, Jia WJ, Duan Q, Ma LL, Jiang XL, Wang JH (2014) Analysis of genetic relationships and identification of lily cultivars based on inter-simple sequence repeat markers. Genet Mol Res 13:5778–5786CrossRefPubMedGoogle Scholar
  8. Duncan G (1996) Growing South African bulbous plants, National Botanical Institute. Cape Town, S. Africa. ISBN 1-874907-15-3Google Scholar
  9. Duncan G (2000) Grow bulbs: a guide to the species cultivation and propagation of South African Bulbs, National Botanical Institute, Kirstenbosch Gardening Series. Claremont, S. Africa. ISBN 0-900048-53-0Google Scholar
  10. Earl DA, vonHoldt BM (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4:359–361CrossRefGoogle Scholar
  11. Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620CrossRefPubMedGoogle Scholar
  12. Flower Council of Holland (2008) Facts and figures. http://www.flowercouncil.org/us/marketinformation/. Accessed 14 June 2009
  13. Goldblatt P (1996) Gladiolus in tropical Africa: systematics, biology & evoluation. Timber Press, Portland, Oregon. ISBN 10: 0-8819-233-38/0-88192-333-8Google Scholar
  14. Goldblatt P, Manning J (1998) Gladiolus in Southern Africa: timber press. Portland. ISBN 10: 1874950326Google Scholar
  15. Goldblatt P, Takei M, Razzaq ZA (1993) Chromosome cytology in tropical African gladiolus (Iridaceae). Ann Mo Bot Gard 80:461–470CrossRefGoogle Scholar
  16. Imanishi H (1989) Gladiolus. In: Mastsuo T (ed) Collected data of plant genetic resources. Kodansya. Scientific, Tokyo, pp 1077–1080Google Scholar
  17. Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bull Soc Vaud Nat 44:223–270Google Scholar
  18. Jingang W, Ying G, Daidi C, Shenkui L, Chuanpin Y (2008) ISSR analysis of 26 general species of Gladiolus hybridus Hort J Northeast Agric Univ 15(4):6–10Google Scholar
  19. Kameswari PL, Girwani A, Radh-Rani K (2014) Genetic diversity in tuberose (Polianthes tuberosa L.) using morphological and ISSR markers. Electron J Plant Breed 5(1):52–57Google Scholar
  20. Kiani M, Memariani F, Zarghami H (2012) Molecular analysis of species of Tulipa L. from Iran based on ISSR marker. Plant Syst Evol 298:1515–1522CrossRefGoogle Scholar
  21. Kumar V, Sharma S, Sharma AK, Sharma S, Bhat KV (2009) Comparative analysis of diversity based on morpho-agronomic traits and microsatellite markers in common bean. Euphytica 170:249–262CrossRefGoogle Scholar
  22. Lepcha B, Nautiyal MC, Rao VK (2007) Variability studies in gladiolus under mid hill conditions of Uttrakhand. J Ornam Hortic 10(3):169–172Google Scholar
  23. Manning J, Goldblatt P, Snijman D (2002) The color encyclopedia of Cape Bulbs, Timber Press. Portland. ISBN 9780881925470Google Scholar
  24. Milbourne D, Meyer R, Bradshaw JE, Baird E, Bonar N, Provan J, Powell W, Waugh R (1997) Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato. Mol Breed 3(2):127–136CrossRefGoogle Scholar
  25. Mondini L, Noorani A, Pagnotta MA (2009) Assessing plant genetic diversity by molecular tools. Diversity 1:19–35CrossRefGoogle Scholar
  26. Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci 70:3321–3323CrossRefPubMedPubMedCentralGoogle Scholar
  27. Patra B, Acharya L, Mukherjee AK, Panda MK, Panda MC (2008) Molecular characterization of ten cultivars of Canna lilies (Canna Linn.) using PCR based molecular markers (RAPDs and ISSRs). Int J Integr Biol 2:129–137Google Scholar
  28. Pragya Bhat KV, Misra RL, Ranjan JK (2010) Analysis of diversity and relationships among gladiolus cultivars using morphological and RAPD markers. Indian J Agric Sci 80(90):766–772Google Scholar
  29. Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR finger printing of potato cultivars. Theor Appl Genet 98:107–112CrossRefGoogle Scholar
  30. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959PubMedPubMedCentralGoogle Scholar
  31. Ranjan P, Bhat KV, Mishra RL, Singh SK, Ranjan JK (2010) Genetic relationship of gladiolus cultivars inferred from fluorescence based AFLP markers. Sci Hortic 123:562–567CrossRefGoogle Scholar
  32. Rathod DM, Chawla SL, Ahur TR, Patel MA (2011) Effect of planting time and chamicals on growth, flowering and yield of gladiolus (Gladiolus grandiflorus) cv. American beauty. J Ornam Hortic 14(1&2):24–27Google Scholar
  33. Rohlf FJ (1993) NTSYS-PC: numerical taxonomy and multivariate analysis system. Version 2.02e, Exeter Software, Setauket, New YorkGoogle Scholar
  34. Shufang G, Huijuin FU, Jingang W (2010) ISSR analysis of M1 generation of Gladiolus hybridus Hort. treated by EMS. J Northeast Agric Univ 17(2):22–26Google Scholar
  35. Swaroop K, Janakiram T (2010) Divergence studies in gladiolus. Indian J Hortic 67:352–355Google Scholar

Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  • Veena Chaudhary
    • 1
  • Mukesh Kumar
    • 2
  • Shailendra Sharma
    • 3
  • Navneet Kumar
    • 2
  • Vipin Kumar
    • 2
  • Hemant Kumar Yadav
    • 4
  • Shiveta Sharma
    • 3
  • Ujjwal Sirohi
    • 2
  1. 1.CSSS (PG) CollegeMachhra, MeerutIndia
  2. 2.Sardar Vallabhbhai Patel University of Agriculture and TechnologyMeerutIndia
  3. 3.Chaudhary Charan Singh UniversityMeerutIndia
  4. 4.NBRILucknowIndia

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