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Genetic diversity and structure of Capparis spinosa L. in Iran as revealed by ISSR markers

Abstract

Capparis spinosa L. (caper bush) is an economically and ecologically important perennial shrub that grows across different regions of Iran. In this study, the genetic diversity and population structure of Iranian genepool of C. spinosa is evaluated using Inter Simple Sequence Repeat (ISSR) markers. Using 10 ISSR primers, 387 DNA fragments (bands) were amplified from the genomic DNA of 92 individuals belonging to twenty-one populations of C. spinosa, of which 378 (97.7%) were polymorphic. High level of genetic diversity (percentage of polymorphic loci = 98.2%, h = 0.1382, I = 0.243), high genetic differentiation (Gst = 0.5234) and low gene flow (Nm = 0.4553) among populations were observed. Caper bush populations were divided into 4 groups in the dendrogram, PCoA plot and Bayesian clustering results, mostly corresponded to their geographic regions. The results showed that there are value in sampling Iranian caper bush populations to look for valuable alleles for use in plant breeding programs.

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Acknowledgements

This research was a part of the Ph.D. thesis of the first author. We wish to thank the Office of Graduate Studies of the University of Isfahan (Iran) for their support. The authors are grateful to the two anonymous reviewers for their valuable comments on the manuscript.

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Correspondence to Hojjatollah Saeidi.

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Ahmadi, M., Saeidi, H. Genetic diversity and structure of Capparis spinosa L. in Iran as revealed by ISSR markers. Physiol Mol Biol Plants 24, 483–491 (2018). https://doi.org/10.1007/s12298-018-0518-3

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Keywords

  • Caper bush
  • Genetic diversity
  • Iran
  • ISSR