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Acetate-induced modulation of ascorbate: glutathione cycle and restriction of sodium accumulation in shoot confer salt tolerance in Lens culinaris Medik.

Abstract

Physiological and biochemical changes in six-day-old hydroponically grown lentil seedlings exposed to 100 mM salinity stress with or without 5 and 10 mM Na-acetate were studied. Results showed that salt stress reduced recovery percentage, fresh weight (FW), chlorophyll (chl) content, disturbed water balance, disrupted antioxidant defense pathway by decreasing reduced ascorbate content, and caused ion toxicity resulting from increased Na+ accumulation, severe K+ loss from roots in hydroponic culture. However, exogenous application of Na-acetate improved the seedling growth by maintaining water balance and increasing chl content. Furthermore, Na-acetate application reduced oxidative damage by modulating antioxidant defense pathway, and sustained ion homeostasis by reducing Na+ uptake and K+ loss. In the second experiment in glass house, we investigated the role of Na-acetate on lentil for long-term salinity. Acetate application increased FW and dry weight, reduced oxidative and membrane damage, and lowered the accumulation of Na+ in shoot compared with salt stressed seedlings alone. From the results of both experiments, it is clear that the exogenous application of Na-acetate enhanced salt tolerance in lentil seedlings.

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Acknowledgements

This research was funded by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. We thank Mr. Sayed Mohammad Mohsin and Khursheda Parvin, Faculty of Agriculture, Kagawa University, Japan for a critical review and editing the English of the manuscript. We also thank Dr. Md. Motiar Rohman, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh for providing lentil seeds.

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MSH conceived, designed, and performed the experiment and prepared the manuscript. MMHS, and MHMBB actively participated in executing the experiment. MH designed the experiment, analyzed the data and edited the manuscript. MF conceived, designed, and monitored the experiment. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Masayuki Fujita.

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The authors declare that there are no conflicts of interest.

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Supplementary material 1 (DOCX 82 kb)

12298_2018_640_MOESM2_ESM.tif

Chlorophyll content (a) and Proline content (b) under salt stress with or without acetate in experiment I Treatments are the same as described in Fig. 1. Mean (± SD) were calculated from three replicates for each treatment. Values with different letters are significantly different at P≤ 0.05 applying Fisher’s LSD test (TIFF 155 kb)

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Hossain, M.S., Hasanuzzaman, M., Sohag, M.M.H. et al. Acetate-induced modulation of ascorbate: glutathione cycle and restriction of sodium accumulation in shoot confer salt tolerance in Lens culinaris Medik.. Physiol Mol Biol Plants 25, 443–455 (2019). https://doi.org/10.1007/s12298-018-00640-6

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Keywords

  • Ion toxicity
  • Lentil
  • Oxidative stress
  • Recovery
  • Salinity
  • Na-acetate