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Responses of wheat plants to interactions of 24-epibrassinolide and Glomus mosseae in saline condition

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Abstract

This study was designed to investigate the possible effects of 24-Epibrassinolide (BR), arbuscular mycorrhizal (AM) fungus, Glomus mosseae, singularly and collectively under salt stress in wheat (Triticum aestivum L.) plants. After foliar spraying of mycorrhizal and non-mycorrhizal plants by 5 µM epibrassinolide (24-Epi), they were treated with 0 and 150 mM NaCl for 2 weeks and then harvested. The results showed interactions of G. mosseae and 24-Epi could alleviate the adverse effects of salinity by improving relative water content (RWC) of leaves (62%), relative growth rate (40.74%), shoot fresh weights (39.83%) and shoot phosphorous content (63.93%), stimulating leaf enzymatic antioxidant activities including catalase (2.24 fold) and ascorbate peroxidase (2.18 fold) as well as malondialdehyde (36.17%) and H2O2 concentrations (49.74%) as compared to those of NaCl treatments. Moreover, mycorrhizal dependency of root dry weight (2%) and phosphorus concentration (0.4%) increased with AM infection and 24-Epi application under saline condition. Leaf RWC, also, negatively correlated with membrane electrolyte leakage. Furthermore, the greatest mitigating effects were observed in mycorrhizal plants subjected to NaCl and 24-Epi. This study indicated that 24-Epi application and AM fungi may synergistically mitigate harmful impacts of salinity in wheat plants.

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Authors and Affiliations

  1. Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, 15719-14911, Iran

    Cobra Tofighi, Ramazan Ali Khavari-Nejad & Farzaneh Najafi

  2. Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Ramazan Ali Khavari-Nejad

  3. National Institute of Genetic Engineering and Biotechnology, Shahrak-e Pajoohesh, Km 15, Tehran, 1417863171, Iran

    Khadijeh Razavi

  4. Soil and Water Research Institute, Agriculture Research Education and Extension Organization, P.O. Box: 31758-311, Imam Khomeini Blv., Meshkindasht, Karaj, Iran

    Farhad Rejali

Authors
  1. Cobra Tofighi
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  2. Ramazan Ali Khavari-Nejad
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  3. Farzaneh Najafi
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  4. Khadijeh Razavi
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  5. Farhad Rejali
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Correspondence to Cobra Tofighi.

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Cobra Tofighi: Principal investigator.

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Tofighi, C., Khavari-Nejad, R.A., Najafi, F. et al. Responses of wheat plants to interactions of 24-epibrassinolide and Glomus mosseae in saline condition. Physiol Mol Biol Plants 23, 557–564 (2017). https://doi.org/10.1007/s12298-017-0439-6

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  • DOI: https://doi.org/10.1007/s12298-017-0439-6

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