Mitigation by sodium nitroprusside of the effects of salinity on the morpho-physiological and biochemical characteristics of Rubus idaeus under in vitro conditions

Ghadakchiasl, Ali; Mozafari, Ali-akbar; Ghaderi, Nasser

doi:10.1007/s12298-016-0396-5

Research Article
Published: 28 November 2016

Mitigation by sodium nitroprusside of the effects of salinity on the morpho-physiological and biochemical characteristics of Rubus idaeus under in vitro conditions

Ali Ghadakchiasl¹,
Ali-akbar Mozafari¹ &
Nasser Ghaderi¹

Physiology and Molecular Biology of Plants volume 23, pages 73–83 (2017)Cite this article

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Abstract

This study examined the changes brought about by sodium nitroprusside (SNP) in the effects of salinity on the morpho-physiological and biochemical characteristics of Rubus idaeus var. Danehdrosht. Raspberry shoot-tip explants were cultured on Murashige and Skoog medium supplemented with a growth regulator that combined benzyleadenine (1 mg/l), indol-3-butyric acetic acid (0.2 mg/l), SNP (0, 50 and 100 µM) and sodium chloride (0, 50 and 100 mM). The results showed that salinity stress significantly decreased morpho-physiological and biochemical characteristics such as RWC, MSI and total protein content in regenerated explants and significantly increased the total soluble sugar, proline contents, peroxidase and superoxide dismutase activity in compared to the control. However, SNP treatments mitigated the impacts of salinity on morphological and physiological characteristics in raspberry shoot-tip explants by increasing the accumulation of proline content, total protein content and total soluble sugar in line with increasing antioxidant enzyme activity under salinity conditions.

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Department of Horticultural Sciences, Faculty of Agriculture, University of Kurdistan, P. O. Box 416, Sanandaj, 66177-15175, Iran
Ali Ghadakchiasl, Ali-akbar Mozafari & Nasser Ghaderi

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Ali Ghadakchiasl
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Ali-akbar Mozafari
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Nasser Ghaderi
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Correspondence to Ali-akbar Mozafari.

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Ghadakchiasl, A., Mozafari, Aa. & Ghaderi, N. Mitigation by sodium nitroprusside of the effects of salinity on the morpho-physiological and biochemical characteristics of Rubus idaeus under in vitro conditions. Physiol Mol Biol Plants 23, 73–83 (2017). https://doi.org/10.1007/s12298-016-0396-5

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Received: 19 July 2016
Revised: 01 October 2016
Accepted: 18 November 2016
Published: 28 November 2016
Issue Date: January 2017
DOI: https://doi.org/10.1007/s12298-016-0396-5

Keywords

Nitric oxide
Raspberry
Salinity stress
In vitro condition